Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"nuclear extract"”.
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DNA-protein interactions throughout the Arabidopsis calmodulin-3 gene
… in the calmodulin-3 gene and the types of nuclear factors with which they interact with the goal of defining the molecular mechanisms regulating calmodulin-3 transcription. The $-$685 to $-$385 region of the calmodulin-3 promoter interacts with abundant A/T-sequence binding nuclear factors, …
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Tissue-Specific Expression and Steroid Hormone Regulation of Human Gonadotropin -Releasing Hormone (hGnRH) Gene in Placental Cells (JEG -3 cells)
… contains four elements, which can bind with nuclear extract from JEG-3 cells (but not from GT1-7 cells).</p> <p>Estradiol (E2) represses the hGnRH upstream promoter activity in JEG-3 cells. This inhibition is receptor-mediated, dose-dependent and promoter-specific. The DNA sequence between …
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Isolation and Characterization of Estrogen Receptor- Interacting Proteins
… a variety of physiological roles through its nuclear receptors, estrogen receptors (ERs). The action of ERs is modulated and mediated by diverse interacting cofactors. The initial aim of this thesis work was to identify ligand-, subtype- and/or cell typespecific ER-interacting proteins. I …
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The role of PU.1 and IRF4 interaction in the biology and function of T helper 2 cells
… that PU.1, IRF4 and NFATc2 form a complex in Th2 nuclear extract. We also demonstrated by ChIP assay that IRF4 directly binds the Il10 and Il4 loci in a time dependent manner. The role of these protein-protein and protein-DNA complexes and their contribution towards Th2 heterogeneity will be …
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Transcriptional Regulation Mediated by Human Papillomavirus E2 Protein
… E2-cellular complexes isolated from nuclear extract of induced cell lines revealed specific protein factors that were absent in the negative control and showed E2 interactions with SWI/SNF chromatin remodeling complex, GCN5 histone acetyltransferase, and components of TFIID. These …
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Purification and characterization of a poly(dG).poly(dC)-binding protein from Parenchinus angulosis
… in sea urchin (Parenchinus angulosis) embryonic nuclear extracts (J.P. Hapgood, personal communication). suGF1 may be involved in the regulation of early histone gene expression by interaction with a nudeosome which has been shown to be positioned in vitro over the H1-H4 intergenic region of the …
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An assessment of the effects of novel anti-inflammatory compounds in cell based studies
… also reflected in the significant reduction of nuclear extract NF-κB-DNA binding activity as detected by Electrophoresis Mobility Shift Assay (EMSA), but without affecting the degradation of IκBα protein induced by TNF-α. Furthermore, AIK18/70 also decreased TNF-α- induced NF-κB-DNA binding …
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Decoding 5HMC as an Active Chromatin Mark in the Brain and its Link to Rett Syndrome
… of the study, we discover a complex in the brain nuclear extract that assembles specifically in the presence of 5hmC. We purify and identify components of this complex: the purine-rich element binding protein (Pur) α and β, which are required for the proper development of neuronal cell types. We …
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The Role of ATP and Cleavage Factor Phosphorylation in pre-mRNA 3' Cleavage Reaction
… cleavage activity to dephosphorylated HeLa cell nuclear extract (NxT). It was determined that immunopurified Flag epitope-tagged-CFIm25, -Clp1, -CFIm68, -CFIm59, and tag-independent immunopurified Pcf11, when used in place of CFm, were not capable of restoring 3' cleavage activity to …
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Metabolic Regulation of Gene Expression through Differential Histone Acylation: The Regulation and Function of Histone Crotonylation
… histone crotonyltransferase (HCT) activity from nuclear extract by fractionation, which resulted in the purification of p300, a well-studied transcriptional co-activator and histone acetyltransferase (HAT). Together with colleagues in the Roeder lab, we established that p300's HCT activity …
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The Regulation of Alkaline Phosphatase during the Development of Dictyostelium
… with the 20% ammonium sulfate (AS) slug nuclear fraction. These DNA-binding activities appear to be related as they all require magnesium or calcium for effective binding to the <i>alp</i> promoter. Interestingly, the DNA-binding proteins appeared to interact with a GT-rich sequence that …
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Purification and Characterization of a Methyl-DNA Binding Protein Complex from Primary Erythroid Cells
… promoter and proximal transcribed region in nuclear extracts from definitive erythrocytes. This complex has a stronger binding affinity for its cognate binding sequence, the methylated ρ-globin proximal transcribed region (M-ρ248), than for an artificial 5-methylcytosine-rich sequence …
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The effect of inhibiting KPNB1-mediated nuclear import on cancer cell biology and inflammatory transcription factor signalling
… in our laboratory and others identified the nuclear import protein, Karyopherin β1 (KPNB1), to be overexpressed in various cancers and that inhibiting its expression blocks the proliferation of cancer cells. However, little is known about the potential role of KPNB1 in other cancer cell …
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The role of topoisomerase II in amsacrine resistance
… breaks in whole L1210 cells, isolated nuclei and nuclear extract systems correlated with drug-induced cytotoxicity, at least for the drugs belonging to the amsacrine lineage. In contrast, there was no direct relationship between the inhibition of topoisomerase II strand-passing activity and the …