Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"nuclear export signal"”.
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Implications of a CALM-derived Nuclear Export Signal for CALM-AF10-mediated Leukemogenesis
… We determine that CALM contains a functional nuclear export signal (NES) that mediates steady-state cytoplasmic localization of CALM-AF10. An NES is a highly conserved leucine-rich amino acid sequence that is recognized by the nuclear export receptor, CRM1. Classically, CRM1 binds to …
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Nuclear Export Signal Recognition by CRM1 Carrying the Oncogenic E571K Mutation and Structure-Based NES Prediction
Nuclear-cytoplasmic trafficking is an essential cellular process in eukaryotes that maintains and regulates the spatial distribution of key cellular processes in the nucleus and the cytoplasm. CRM1 is the major nuclear export mediator, which facilitates nuclear export of hundreds of protein and RNA …
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Studies on the interaction of HSV-1 multifunctional protein ICP27 with the nuclear pore complex proteins
… levels. ICP27 contains both a nuclear localization signal and a nuclear export signal and shuttles between the nucleus and the cytoplasm. It interacts directly with various cellular proteins including REF and cellular mRNA export receptor TAP to export viral mRNAs. However other …
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Molecular Mechanism of Insulin Resistance: Role of mTOR Signaling Pathways
… IV, I describe another investigation of mTOR signaling mechanisms. In this study I initially set out to identify nuclear export signals in mTOR. A systematic scan of the mTOR sequence revealed 16 peptides conforming to the canonical leucine-rich nuclear export signal, of which 3 were found by …
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Nuclear accumulation of G-actin in cellular senescence: Reduced expressions of exportin 6 and Ran small G protein
… protein, which contains two leucine-rich nuclear export signal (NES) sequences in the middle of the molecule. It has been known that actin is exclusively kept in the cytoplasm by exportin 1 (CRM1)-driven nuclear export. However, CRM1 has been reported to be not directly involved in actin …
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THE ROLE OF TIGHT JUNCTION PROTEIN ZO-2 IN THE NUCLEUS AND ITS LINK TO TRANSCRIPTION FACTORS OF THE SLUG/SNAIL FAMILY
… factor was shown to possess functional nuclear localization signals, nuclear export signal and zinc fingers that are important for DNA binding and nuclear retention. Furthermore, ZO-2 may also influence the sub-cellular localization of this transcription factor, for example in wound …
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Mechanistic and Physiological Studies of the Insulin-Dependent Regulation of FOXA2
… fasting conditions, but is inhibited by insulin signaling via PI3K/Akt in a phosphorylation-dependent manner, which results in its nuclear exclusion. However, the mechanism and relative importance of nuclear export have not yet been elucidated. In addition, the existence and potential role of …
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Regulation of the Intracellular Trafficking of Ajuba by Cell Density and Cell Cycle-Dependent Mechanisms
… LIM domain plays an essential role in its nuclear localization, while a nuclear export signal (NES) in its preLIM domain guides its exit from the nucleus to the cytoplasm. The essential role of the NES signal was underlined by insights into Ajuba variant 1 and its response to Leptomycin B …
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Analysis of nuclear transport signals of the human apurinic/apurimidinic endonuclease (APE1/REF1)
The nuclear localization signal (NLS) in human apurinic/apyrimidinic endonuclease (APE1), a key protein in both DNA base excision repair and transcriptional regulation, has not been analyzed in detail. We examined the role of specific residues in nuclear translocation of APE1, using green …
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Charakterisierung kernständiger Funktionen der NAD+-abhängigen Deacetylase SIRT2
… lysine-16 of histone 4. Due to the sufficient nuclear export of SIRT2 its function inside the nucleus was difficult to elucidate. The aim of this study was to describe nuclear functions of SIRT2 without using agents to block nuclear export. Therefore a SIRT 2 mutant was generated which remains …
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RNA Dysregulation in a Novel Human Model of TDP43 Proteinopathy
… present with TDP43 pathology. This involves the nuclear-to-cytoplasmic mislocalisation and aggregation of TDP43, an important nuclear regulator of mRNA splicing. In this thesis, we describe a novel in vitro model allowing on-demand mislocalisation of endogenous TDP43, without its mutation or …
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Role of Sub-Cellular Localization and Cellular Factors In Regulation of HIV-1 Tat Protein Stability
… is active both in the cytoplasm and nucleus. Nuclear and cytoplasmic protein extracts prepared following CHX treatment showed marked degradation of Tat in cytoplasm whereas nuclear Tat showed minimal degradation. Degradation pattern of cytoplasmic Tat mutants, one lacking nuclear localization …
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Recognition Mechanisms of Nuclear Localization and Export Signals
… recognize their substrates through either a nuclear localization or export signal depending on the direction of transport. Even though there are ten yeast import Karyopherin betas, for the past thirteen years there was only one well characterized nuclear localization signal, the classical …
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Die Funktion von NLP1 im CRM1-abhängigen Protein-Export aus dem Zellkern
Der Export der meisten Proteine aus dem Zellkern ins Cytosol erfolgt unter Beteiligung des Exportfaktors CRM1. Dieser erkennt einen hydrophoben Bereich des zu exportierenden Proteins (NES = nuclear export signal), bindet ihn in Gegenwart von RanGTP und vermittelt anschließend den Durchtritt durch …
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The functional motifs for nucleocytoplasmic shuttling of C-terminal fragment of c-Met : A novel "pH-dependent' nuclear localization signal
… specific amino acid motif nor the responsible nuclear localization signal (NLS) has not been previously reported. Here, we report that two histidine residues separated by a 10-amino-acid spacer (H1068-H1079) located in the juxtamembrane region of c-Met function as a putative novel NLS. …
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Regulation of human protein kinases and phosphatases by 14-3-3
… FAM122A to the nucleus, perhaps by masking the nuclear export signal (NES) that I identified. Another possibility is that 14-3-3 binding exposes a nuclear localisation signal (NLS) that was identified on FAM122A, and whose mutation promotes cytoplasmic localisation of the protein.<br/><br/>The …
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An investigation into the role of ubiquitination in plant immunity
… in Arabidopsis. The identification of AtPUB17 signalling partners would allow to understand the mode of action of AtPUB17 during plant defence. AtPOB1, a BTB/POZ-domain protein was isolated as an AtPUB17 interactor in a yeast-two-hybrid screen. The aim of this study was to confirm this …
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Nuclear export signals (NESs) in Arabidopsis thaliana : development and experimental validation of a prediction tool
… between nucleus and cytoplasm lies in its nuclear export activity. The widely used mechanism for export of proteins from the nucleus involves the receptor Exportin 1 and the presence of a nuclear export signal (NES) in the cargo protein. Given the big amount of sequence data available …
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Identification and characterization of Risp
… protein), because it shuttles between the nuclear and the cytoplasmic compartments. The Risp gene is widely expressed in human cells and conserved among various species, most probably as part of a larger gene. High amino acid homology (99%) with the C-terminal part of a large brain cDNA …
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Analysis of SMAUG1 protein motifs and interacting partners - linking regulation of phase separation to human disease
… condensate dissolution. This work establishes a signalling dependent mechanism for dynamic control of SMAUG1 phase behaviour and with findings published as a first-author manuscript. In Chapter 3, we tested SMAUG1 condensation in vitro and defined its intrinsic phase separation behaviour. …
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