Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 88 for “"nuclear export"”.
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Nuclear Export Receptor CRM1 Recognizes Nuclear Export Signals with Diverse Conformations
… of Maintenance 1 or CRM1 protein facilitates export of hundreds of proteins and RNA molecules from eukaryotic cell nuclei. CRM1 recognizes its protein cargoes by their 8-15 residues-long nuclear export signals or NESs, which bind to a hydrophobic groove in CRM1. NESs are highly variable in …
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Mechanisms of Human LARP6 Nuclear Export
… supported by the identification of a putative nuclear export sequence (NES) and to localization in both the nucleus and the cytoplasm. Subsequent high-resolution structures of the RNA binding domain of LARP6 showed that the NES is located in a core part of the RNA Recognition Motif, where it …
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The Influenza A Virus NS1 Protein and Vriral mRNA Nuclear Export
… both the viral genome (vRNA) and mRNA must be exported from the nucleus to the cytoplasm. The mechanism by which vRNA nuclear export is achieved has been well characterised, but how viral mRNAs are exported is poorly understood. The cellular NXF1-dependent mRNA export pathway has been shown to …
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Implications of a CALM-derived Nuclear Export Signal for CALM-AF10-mediated Leukemogenesis
… We determine that CALM contains a functional nuclear export signal (NES) that mediates steady-state cytoplasmic localization of CALM-AF10. An NES is a highly conserved leucine-rich amino acid sequence that is recognized by the nuclear export receptor, CRM1. Classically, CRM1 binds to …
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Nuclear export signals (NESs) in Arabidopsis thaliana : development and experimental validation of a prediction tool
… between nucleus and cytoplasm lies in its nuclear export activity. The widely used mechanism for export of proteins from the nucleus involves the receptor Exportin 1 and the presence of a nuclear export signal (NES) in the cargo protein. Given the big amount of sequence data available …
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Nuclear Export Signal Recognition by CRM1 Carrying the Oncogenic E571K Mutation and Structure-Based NES Prediction
Nuclear-cytoplasmic trafficking is an essential cellular process in eukaryotes that maintains and regulates the spatial distribution of key cellular processes in the nucleus and the cytoplasm. CRM1 is the major nuclear export mediator, which facilitates nuclear export of hundreds of protein and RNA …
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p53 nuclear localization control, and p53-dependent regulation of DNA repair gene transcripts
… genes. Chapter 2 addresses the regulation of nuclear localization of the p53 protein. Previous reports in the literature had suggested that the p53 negative regulator HDM2 was a nucleocytoplasmic shuttling protein that binds and carries p53 from the nucleus of the cell to the cytoplasm where …
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Molecular Mechanism of Insulin Resistance: Role of mTOR Signaling Pathways
… In this study I initially set out to identify nuclear export signals in mTOR. A systematic scan of the mTOR sequence revealed 16 peptides conforming to the canonical leucine-rich nuclear export signal, of which 3 were found by reporter assays to contain LMB-sensitive and leucine-dependent …
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Nuclear accumulation of G-actin in cellular senescence: Reduced expressions of exportin 6 and Ran small G protein
… protein, which contains two leucine-rich nuclear export signal (NES) sequences in the middle of the molecule. It has been known that actin is exclusively kept in the cytoplasm by exportin 1 (CRM1)-driven nuclear export. However, CRM1 has been reported to be not directly involved in actin …
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Role of Sub-Cellular Localization and Cellular Factors In Regulation of HIV-1 Tat Protein Stability
… is active both in the cytoplasm and nucleus. Nuclear and cytoplasmic protein extracts prepared following CHX treatment showed marked degradation of Tat in cytoplasm whereas nuclear Tat showed minimal degradation. Degradation pattern of cytoplasmic Tat mutants, one lacking nuclear localization …
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Mechanistic and Physiological Studies of the Insulin-Dependent Regulation of FOXA2
… manner, which results in its nuclear exclusion. However, the mechanism and relative importance of nuclear export have not yet been elucidated. In addition, the existence and potential role of insulin-dependent regulation of Foxa2 have not been studied in other tissues where it …
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Identification of Oncogenic KRAS-Associated Vulnerabilities in Non-Small Cell Lung Cancer
… analysis. This identified components of nuclear transport machinery as selectively essential for KRAS mutant NSCLC lines. We found that pharmacological inhibition of a key nuclear export receptor, XPO1 (a.k.a. CRM1), was sufficient to induce robust and selective apoptosis in KRAS mutant …
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Structural characterization of a protein/RNA complex : human TAP/NXF1 protein/retroviral CTE RNA
… processing of pre-mRNA transcripts and export of the correctly processed mRNAs from the nucleus to the cytoplasm, where translation takes place. Evidence has accumulated suggesting that these steps are both functionally and physically coupled by protein-protein interactions, involving …
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Cellular Functions of the Squash Leaf Curl Geminivirus Movement Proteins BL1 and BR1
… the nuclei of infected cells and functions as a nuclear shuttle protein. BL1 is directly responsible for viral pathogenic properties, and transient expression assays in tobacco protoplasts demonstrated that BL1 provides directionality to virus movement by cooperatively interacting with BR1 to …
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Charakterisierung kernständiger Funktionen der NAD+-abhängigen Deacetylase SIRT2
… lysine-16 of histone 4. Due to the sufficient nuclear export of SIRT2 its function inside the nucleus was difficult to elucidate. The aim of this study was to describe nuclear functions of SIRT2 without using agents to block nuclear export. Therefore a SIRT 2 mutant was generated which remains …
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FROM NUCLEUS TO MITOCHONDRIA: INSIGHTS INTO THE ROLE OF TERT IN THE PROGRESSION OF PAPILLARY THYROID CARCINOMA
… work we aimed to study the role of TERT extra-nuclear localization in the progression of PTC. We assessed TERT nuclear export, resistance to OS and proliferative properties in the K1 papillary thyroid cancer immortalized cell line model transfected with wild-type TERT and specific TERT plasmid …
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Determinants of piRNA precursor transcription and export in Drosophila melanogaster
… These features are essential for canonical mRNA export mediated by Nuclear export factor 1, suggesting that piRNA precursor export requires an alternative machinery. In the second part of this thesis, I show that dual-strand cluster precursor export requires the Drosophila Nuclear export factor …
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The Study of Wnt Signaling Effector POP-1/TCF in C. Elegans Early Embryos
… anterior cells. This phenomenon is called POP-1 nuclear asymmetry. The Wnt/MAPK signaling is required for POP-1 nuclear asymmetry. It is believed that POP-1 represses endoderm fate in MS and Wnt/MAPK allows endoderm fate in E by downregulating the nuclear level of POP-1. In this study, the …
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Investigating Mechanisms of Nuclear mRNA Export
… in the cytoplasm has important implications for nuclear export and retention of mRNAs. This division ensures that only correctly processed mRNAs are exported to the cytoplasm to be translated into proteins, while misprocessed and aberrant transcripts are retained in the nucleus to be degraded by …
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Subzelluläre Lokalisation des Y-box-Proteins-1 (YB-1) in Mesangialzellen und Charakterisierung der dafür verantwortlichen Proteindomänen
… Within one day YB-1 localizes to the perinuclear region of cells within all glomeruli . In the course of the disease the strongest cytoplasmic YB-1 signal coincided with the peak mesangial cell activation/proliferation between days 4 and 6 and was detected in a mesangial distribution …
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