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Showing 1 to 16 of 16 for “"nuclear envelope (NE)"”.
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Discrete 3D Model of Molecular Diffusion Through the Nuclear Pore Complex
Nuclear pore complexes (NPCs) are passageways that exist within the nuclear envelope (NE) of a eukaryotic cell. Molecular cargo travel through the passageways to either import to the nucleus or export to the cytoplasm of the cell. Efficient export of certain cargo is necessary for maintained health …
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Confocal and electron microscopy investigations of the plant endoplasmic reticulum–nuclear envelope interface and meristematic cell divisions
The plant endoplasmic reticulum (ER) and nuclear envelope (NE) are central in the production and transport of proteins, and additionally play vital roles in cell division. However, many structural and functional aspects remain unexplored in plants. The ER is composed of tubules and cisternae, …
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Myosin-Like Proteins in S. cerevisiae: Multifunctional, Structural Components of the Nuclear Envelope
<p>The nuclear envelope (NE) separates the genetic material from the rest of the cell, delimits and defines the nucleus, organizes the intranuclear architecture and serves as a regulator for multiple nuclear processes. In all eukaryotes, filamentous coiled-coil proteins are associated with the …
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Characterisation of Nuclear EnvelopeAssociated Proteins (NEAP) in Arabidopsis thaliana
… evolution, eukaryotic cells have acquired a nuclear envelope (NE) enclosing and protecting the genome, which is organized in chromatin, a structure wrapping DNA around histone proteins. The NE is composed of two membranes: on the nucleoplasmic side, the Inner Nuclear Membrane (INM) and on the …
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Core Components Of The Nuclear Pore Bind Unique States Of Chromatin And Mediate Polycomb Repression
While the Nuclear Pore Complex’s (NPC) canonical function is to act as a selective barrier across the Nuclear Envelope (NE) and regulate nucleocytoplasmic traffic, recent studies have shown that Nucleoporins (Nups), the 30 proteins that comprise the NPC, are also involved in chromatin-related …
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Production, characterisation and use of fluorescent markers in the study of plant nuclear envelope dynamics
The nuclear envelope (NE) is one of the least studied membranes in plant cells. Genes encoding NE protein homologues are absent from currently available sequenced plant genomes. To produce a specific marker for the plant NE and in view of previous positive results with heterologously expressed …
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Characterisation of the Arabidopsis mid-SUN proteins at the plant NE
… and Cytoskeleton (LINC) complex formation at the nuclear envelope (NE), similarly to Cter-SUN domain proteins. In addition to interacting with LINC complex components, they have been reported to localise to both the NE and endoplasmic reticulum (ER). There is also evidence that they interact with …
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Novel plant nuclear envelope-associated coiled-coil proteins
The nuclear envelope (NE) is a double lipid bilayer enclosing the eukaryotic genome. The metazoan nucleoskeleton includes the peripheral lamina and the internal nucleoskeleton. The lamina is composed of a network of intermediate filament (IF) proteins called lamins, as well as lamin- and/or …
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Proteomic, Bioinformatic and Functional Characterization of the Nuclear Pore Complex of the African Trypanosome
… within the boundary of a double membrane, known as the nuclear envelope (NE). Transport across the NE is mediated by large protein assemblages known as nuclear pore complexes (NPCs). Yeast and vertebrate NPCs are comprised of about 30 proteins, termed nucleoporins (Nups), which are …
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Structural and biochemical characterization of LINC complexes
The nuclear envelope (NE) is comprised of a double membrane bilayer that physically separates the nucleoplasm from the cytoplasm. Information can be transmitted through the NE by molecular exchange through the nuclear pore complex (NPC) and by transduction of mechanical forces mediated by the …
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Identification and Characterization of Mlp1p and Mlp2p : Molecular Components of Filaments Localized at the Interface between the Nuclear Pore Complex and Nuclear Interior
… the segregation of the genome in a closed organelle requires an efficient mechanism to ensure the constant exchange of material between the nucleus and the cytoplasm. Nuclear pore complexes (NPCs) provide the only known sites for exchange of material across the nuclear envelope (NE). A wealth …
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Characterisation of the multi-isomeric protein nesprin-1 in p-body and mRNA dynamics
Nuclear envelope spectrin repeat proteins, or Nesprins, are a novel family of nuclear and cytoskeletal proteins with rapidly expanding roles as intracellular scaffolds and linkers. Nesprins are characterized by a central spectrin repeat (SR) rod domain and a C-terminal KASH domain, which acts as a …
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Biophysical and structural characterization of components from the nuclear pore complex and the ubiquitin pathway
Formation of an endomembrane system in the eukaryotic cell is a hallmark of biological evolution. One such system is the nuclear envelope (NE), composed of an inner and outer membrane, used to form a nucleus and enclose the cell's genome. Access to the nucleus from the cytoplasm is mediated by a …
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Analyzing the role of a cellular mechanism in HIV infection and Evaluation of novel antiviral compounds
… twenty years of intense research still no vaccine or curing therapy has been found. For the development of novel antiviral compounds, understanding of the HIV lifecycle as well as the molecular mechanisms of host pathogen interactions are a prerequisite. Like all viruses, HIV subcontracts …
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Molecular Size and Charge Effects on Nucleocytoplasmic Transport Studied By Single-Molecule Microscopy
In eukaryotic cells, essential genetic information is sequestered in the prominent organelle nucleus. To facilitate the selective bidirectional trafficking of genetic material, proteins and ribonucleoprotein cargoes across the nuclear envelope (NE), nucleus has evolved the highly sophisticated …
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NUCLEAR ENVELOPE TRANSMEMBRANE PROTEIN DISTRIBUTION AND TRANSPORT STUDIED BY SINGLE-MOLECULE MICROSCOPY
… of eukaryotic cells is a vitally important organelle that sequesters the genetic information of the cell, and protects it with the help of two highly evolved structures, the nuclear envelope (NE) and nuclear pore complexes (NPCs). Together, these two structures mediate the bidirectional …