Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 98 for “"nuclear envelope"”.
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Novel plant nuclear envelope-associated coiled-coil proteins
The nuclear envelope (NE) is a double lipid bilayer enclosing the eukaryotic genome. The metazoan nucleoskeleton includes the peripheral lamina and the internal nucleoskeleton. The lamina is composed of a network of intermediate filament (IF) proteins called lamins, as well as lamin- and/or …
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How Chromosome-Nuclear Envelope Attachments Affect 3D Genome Organization
… folding and influence gene regulation, the nuclear envelope is expected to play a significant role since it serves as the physical boundary preventing chromosome from freely diffusing in the cell cytosol. Moreover, experiments suggest that the nuclear envelope engages chromosomes actively by …
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Binding sites in the nuclear envelope for cytoplasmic glucocorticoid receptor complex
… radioactive peak on a 5 - 20% sucrose gradient. Nuclear envelopes isolated from purified rat liver nuclei were found to contain binding sites for the partially purified cytoplasmic triamcinolone acetonide-receptor complex. The binding constants showed two saturable high affinity binding sites and …
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NUCLEAR ENVELOPE TRANSMEMBRANE PROTEIN DISTRIBUTION AND TRANSPORT STUDIED BY SINGLE-MOLECULE MICROSCOPY
… the help of two highly evolved structures, the nuclear envelope (NE) and nuclear pore complexes (NPCs). Together, these two structures mediate the bidirectional trafficking of molecules between the nucleus and cytoplasm by forming a barrier. NE transmembrane proteins (NETs) embedded in either …
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Myosin-Like Proteins in S. cerevisiae: Multifunctional, Structural Components of the Nuclear Envelope
<p>The nuclear envelope (NE) separates the genetic material from the rest of the cell, delimits and defines the nucleus, organizes the intranuclear architecture and serves as a regulator for multiple nuclear processes. In all eukaryotes, filamentous coiled-coil proteins are associated with the …
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Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases
… 2.2.1), and the in vitro effect of insulin on nuclear envelope phosphorylation, NTPase activity, and mRNA efflux (see chapter 5, section 5.1). These independent observations have led to the development of a model for the direct induction by insulin, at the level of the nucleus, of mRNA efflux …
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Production, characterisation and use of fluorescent markers in the study of plant nuclear envelope dynamics
The nuclear envelope (NE) is one of the least studied membranes in plant cells. Genes encoding NE protein homologues are absent from currently available sequenced plant genomes. To produce a specific marker for the plant NE and in view of previous positive results with heterologously expressed …
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Novel Intermediate Filaments in the Nuclear Envelope: Molecular Cloning and Analysis of Lamins A and C
… A, B and C are the major polypeptides of the nuclear lamina, the filamentous meshwork intercalated between chromatin and the inner nuclear membrane. Amino acid sequences have been deduced from cDNA clones of human lamins A and C which show identity with the exception of an additional 98 amino …
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Mechanotransduction at the nuclear envelope: the role of forces in facilitating embryonic stem cell fate decisions
… structural and chromatin binding proteins in the nuclear envelope, notably, the lamins (LMNA), Lamin B receptor (LBR) and components of the LINC complex (nesprins/KASH). The structural proteins in the nuclear envelope regulate both the mechanical response of the nucleus to force and the …
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Confocal and electron microscopy investigations of the plant endoplasmic reticulum–nuclear envelope interface and meristematic cell divisions
The plant endoplasmic reticulum (ER) and nuclear envelope (NE) are central in the production and transport of proteins, and additionally play vital roles in cell division. However, many structural and functional aspects remain unexplored in plants. The ER is composed of tubules and cisternae, …
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Study of the role of plant nuclear envelope and lamina-like components in nuclear and chromatin organisation using 3D imaging
… protein bridge connecting the cytoplasmic and nuclear compartments across the nuclear membrane. While recent data supports its function in nuclear morphology and meiosis, its implication for chromatin organisation has been less studied in plants. The fi aim of this work was to develop NucleusJ …
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Microtubule Motors Drive Nuclear Dynamics and Positioning in Developing Skeletal Muscle Cells
… tissue development, and defects resulting in nuclear mispositioning are often associated with human disease, such as muscular dystrophy and myopathy. Skeletal muscle cells are large syncytia formed by fusion of myoblasts, and contain hundreds of nuclei positioned regularly along the length the …
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CELL GEOMETRIC CONSTRAINTS REGULATE NUCLEAR AND CHROMATIN PLASTICITY VIA ACTOMYOSIN CONTRACTILITY
… mechanical signals (EMS) can regulate nuclear morphology and chromatin dynamics via the physical link from focal adhesions to chromatin via the cytoskeleton, linker proteins on the nuclear envelope and the nuclear lamina. However, how such force transmission from cytoskeleton to the …
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Mechanisms of epigenetic regulation in epidermal keratinocytes during skin development. Role of p63 transcription factor in the establishment of lineage-specific gene expression programs in keratinocytes via regulation of nuclear envelope-associated genes and Polycomb chromatin remodelling factors.
… mice about 32% of keratinocytes showed altered nuclear morphology. Alterations in the nuclear shape were accompanied by decreased expression of nuclear lamins (Lamin A/C and Lamin B1), proteins of the LINC complex (Sun-1, nesprin-2/3) and Plectin. Plectin links components of the nuclear envelope …
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Mechanisms of Cytotoxicity and Intracellular Trafficking for Gene Delivery Polymers
… on several mechanisms of cytotoxicity, including nuclear envelope permeabilization, were conducted. Longer polymers induced more cytotoxic responses than shorter ones, and it appears that hydroxyl groups in the repeat unit of polymers play a role in polyplex formation. This research has also led …
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Myogenic Effectors and Disease
… show that constitutive and juvenile loss of the nuclear envelope protein Net39 in mice recapitulates different manifestations of Emery-Dreifuss muscular dystrophy. Deletion of Net39 caused disruption of nuclear envelope integrity and associated genomic, transcriptional, and metabolic changes that …
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The molecular basis of LINC complex formation
… cytoplasm occurs mostly through openings in the nuclear envelope composed of nuclear pore complexes. These massive assemblies allow for regulated transport of macromolecules across the barrier that is the nuclear envelope. However, another means of communication between the nucleus and the …
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Characterisation of Nuclear EnvelopeAssociated Proteins (NEAP) in Arabidopsis thaliana
… evolution, eukaryotic cells have acquired a nuclear envelope (NE) enclosing and protecting the genome, which is organized in chromatin, a structure wrapping DNA around histone proteins. The NE is composed of two membranes: on the nucleoplasmic side, the Inner Nuclear Membrane (INM) and on the …
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RNA localizations of proteins that interact with the chromosome passenger complex
… of their biology, but how mitosis affects these nuclear ncRNA transcripts is less well understood. This study examined the behavior of several ncRNAs through mitosis. It is understood that Aurora B Kinase, or AURKB, plays a role in the dispersal of the ncRNA Xist from chromatin in mitosis, but …
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Investigation of Nuclear Pore Complex Protein Interactions and the Implications for Nuclear Transport
… exchanging proteins and RNA, for a variety of nuclear and cytoplasmic processes which act in concert. The nuclear pore complex is responsible for controlling the transport of large molecules into and out of the cell nucleus. It is perhaps the largest protein structure in eukaryotic cells, and …
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