Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"nuclear architecture"”.
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Regulatory Landscapes Associated With Changes In Nuclear Architecture
… regulation is played by the cell’s chromatin architecture. Gene positioning relative to the center of the nucleus or its three-dimensional contacts with other genomic regions can determine whether the gene is expressed or not. Changes in chromatin architecture have been reported in cell …
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Super-resolution Imaging of Chromatin and Functional Nuclear Architecture
… due to the dense packaging with a small nuclear volume. The nuclei of Drosophila melanogaster primary spermatocytes have unique properties that can circumvent these limitations, with comparatively large nuclei with well-separated chromosome masses, and transcriptionally active Y loops …
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Differentiation Induces Dynamic Alterations in Mesenchymal Stem Cell Nuclear Architecture and Mechanotransduction
… been performed to probe alterations in cell and nuclear architecture in response to physiological loading. In this thesis, we investigated relationships between MSC cellular/nuclear biophysical properties and mechanosensitivity, and determined their importance in MSC mechanotransduction. Our …
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Modelling Nuclear Body Dynamics in Living Cells by 4-D Microscopy, Image Analysis and Simulation
… demonstrates rules of and validates models for nuclear body (NB) dynamics. Simulation tools developed in the course of this work can be used in future work to generate hypotheses about related aspects of nuclear architecture. Initially I examined the mobility of vimentin nuclear bodies bodies …
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Establishing models of and characterising the genomic context of neutrophil hypersegmentation
… of defence against invading pathogens. Their nuclear architecture is highly dynamic, composed of chromatin clustered into lobes that are linked together by dense heterochromatin. Both the underlying mechanism of how neutrophils alter their nuclear architecture and the purpose of their …
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Dynamic organization of chromosomes in the mammalian cell nucleus
Uncovering the motifs of a higher order nuclear architecture and its implications on nuclear function has raised increasing interest in the past decade. The nucleus of higher eukaryotes is considered to display a highly dynamic interaction of DNA and protein factors. There is an emerging view that …
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Integrative analysis of transcriptional activity and genome architecture changes upon viral infections
To study the interplay between spatial nuclear architecture and transcriptional activity during viral infections, I employed a genome-wide chromosome conformation capture approach (Hi-C) on infected murine and human cells and further enriched those libraries for genomic loci of interest and the …
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Mechanisms of epigenetic regulation in epidermal keratinocytes during skin development. Role of p63 transcription factor in the establishment of lineage-specific gene expression programs in keratinocytes via regulation of nuclear envelope-associated genes and Polycomb chromatin remodelling factors.
… mice about 32% of keratinocytes showed altered nuclear morphology. Alterations in the nuclear shape were accompanied by decreased expression of nuclear lamins (Lamin A/C and Lamin B1), proteins of the LINC complex (Sun-1, nesprin-2/3) and Plectin. Plectin links components of the nuclear envelope …
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Modeling Genome Organization in Eukaryotic and Prokaryotic Cells Using Folding Determinant Interactions
… physics from specific interactions mediated by nuclear landmarks and regulatory factors. In this thesis, I develop polymer-based chain-growth Monte Carlo frameworks to investigate genome folding in both mammalian and bacterial systems. For mammalian cells, I model how lamina-associated domains …
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Mechanistic and Epigenetic Partitioning of Lamina-Associated Chromatin Revealed by a Genome-Wide Imaging Screen
The nuclear periphery has long been recognized as a site of heterochromatin organization, where lamina-associated domains (LADs) play a crucial role in transcriptional repression and genome stability. However, the molecular mechanisms that tether LADs in human cells remain poorly defined. To …
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Synaptic and extrasynaptic NMDA receptors in hippocampal neurons: regulation of nuclear shape and cell fate
… calcium signal that invades the cell nucleus. Nuclear calcium is a key regulator of gene expression mediated by the transcription factor CREB. The nuclear architecture contains intranuclear structures that have been shown to facilitate calcium influx into the nucleus. This thesis describes a …
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Novel Roles for A-Type Lamins in Maintaining Genomic Stability
… have been implicated in the maintenance of nuclear architecture and chromatin organization. Mutations in A-type lamins have been associated with defects in a number of nuclear processes, including DNA replication and repair, and gene transcription and silencing. In support of an important …
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Role of chromatin condensates in tuning nuclear mechano-sensing in Kabuki Syndrome
… by exerting mechanical forces that shape nuclear morphology and architecture. The balancing between two chromatin compartments with antagonist functions, namely Transcriptional and Polycomb condensates, is required for preserving nuclear mechanical properties and its perturbation is …
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The genome of Euglena gracilis: Annotation, function and expression
… complexity. Several biological systems such as nuclear architecture (e.g. chromosome segregation, nuclear pore complex, nuclear lamins), protein trafficking, translation, surface, consist of conserved and divergent components. For instance, several gene families likely associated with the cell …
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Requirement and regulation of actin polymerization during endocytosis
… dip1for3 double mutant cells. An excess of nuclear actin leads to defects in nuclear architecture and appears to cause defects in chromosome segregation. This finding establishes a model system to gain further insight into functions of nuclear actin. In summary, this thesis provides insights …
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Multiscale Modeling of Genome Organization: Bridging Polymer Physics, Molecular Dynamics, and AI
… apply computational frameworks to investigate nuclear genome organization at high spatial and temporal resolution. Our approaches integrate biophysical modeling and generative artificial intelligence to address complementary aspects of nuclear architecture. In Chapter 1, we provide an overview …
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Mechanisms of genomic stability maintenance in fission yeast
… parameters affecting the process and among them, nuclear architecture and the 3D genome structure are critical. In the present study, we set out to investigate the implication of the latter parameters in the replication program, using the model organism S. pombe. Combining genome-wide datasets …
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Topology of Genes in Mammalian Cell Nuclei with Special Emphasis on the MLL Gene and Its Translocation Partners
… interphase nucleus of hematopoietic cells. The nuclear localization of a gene relative to the nuclear center and the nuclear periphery was determined. To provide a general understanding of the distribution pattern of a given locus within the nucleus, the nuclear volume was divided into five …
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Chromosome evolution and mechanisms of speciation in the Anopheles gambiae complex
… (Chr-Chr) contacts and chromosome-nuclear envelope (Chr-NE) attachments during the development of the organism, we conducted microscopic analyses of the 3D organization of polytene chromosome in An. gambiae, An. coluzzii, and An. merus. Our quantitative study on chromosome …
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The subnuclear localisation of Notch responsive genes
Title: The subnuclear localisation of Notch responsive genes. Candidate Name: Matthew Jones Notch signalling is a highly conserved cell-cell communication pathway with critical roles in metazoan development and mutations in Notch pathway components are implicated in many types of cancer. Notch is …