Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"novel transcripts"”.
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Identification of novel transcripts in hepatocellular carcinoma using RNA-seq
… technologies have helped the finding of novel transcripts by deep RNA sequencing (RNA-Seq). The approach to identify novel transcripts using RNA-Seq has been studied, however, prediction of their functional roles in cancers are not studied thoroughly. Here, I constructed new pipeline to …
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BIOINFORMATICS ANALYSIS OF FULL-LENGTH RNA NANOPORE SEQUENCING AT SINGLE CELL LEVEL TO STUDY TRANSCRIPTIONAL AND MUTATIONAL PATTERNS IN HUMAN LEUKEMIA
… to technological constraints. To address this, a novel high-throughput multi-omics approach, Single Cell and Molecule sequencing (SCM-seq), was developed. This method integrates droplet-based single-cell RNA sequencing with Nanopore single-molecule sequencing, allowing comprehensive profiling of …
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New Targets of p53 Regulation: From Aging to Immune Response
… molecular biology approaches to further identify novel functions of the universal stress sensor, p53. The first study describes a novel genetic model system to address the effects of p53 dosage in an in vivo context. By developing an allelic series through multiple genetic crosses, I obtained mice …
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Non-Coding Genomics of Methanocaldococcus Jannaschii: A Survey of Promoters, Non-Coding RNA Genes, and Repetitive DNA Elements
… promoters, non-coding RNA genes, and novel repetitive DNA sequences. Genome-wide identification of transcription factor binding sites revealed archaeal core promoter elements, which include TATA box, BRE, and an initiator element. The structural similarity between archaeal basal …
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Transcript Diversity In The Protozoan Parasite Toxoplasma Gondii
… advances have made possible to sequence RNA transcripts at unprecedented depth, enabling deep profiling of abundance and diversity under a variety of conditions. Such information permits refinement of draft genome annotation originally generated in the absence of transcript coverage data, and …
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Noxious Cellular Injury Triggers an Oxidative Stress Gene Response
… downregulated genes were associated with many novel transcripts/proteins such as AC117378, AD000671, and AC023509. Quantitative PCR (qPCR) was used to evaluate several genes identified from RNA-seq data. Cells stimulated with CA venom for 4 hours induced an up-regulation of MT1X (fc: …
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A DE NOVO COMPUTATIONAL DISCOVERY PLATFORM FROM RNA TO PROTEIN
… and functional characterization of human transcripts and proteins remain challenging tasks in the post-genomics era. Transcriptional and post-transcriptional regulation mechanisms hugely increase RNA isoform diversity, while their contribution to protein synthesis remains vastly …
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Isoform-Specific Expression During Embryo Development in Arabidopsis and Soybean
… classifier to assess the coding potential of novel transcripts with respect to RNA secondary structure free energy, conserved domains, and sequence properties. We also examined the effect of alternative splicing on the domain composition of resulting protein isoforms. The majority of splice …
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Transcriptional and developmental consequences of aneuploidy during male meiosis
… elements in the Tc1 mouse, this suggests that novel transcripts arising from human repeats can trigger an adaptive piRNA response, thereby demonstrating the plasticity of this pathway to newly invading repeat elements. Transcriptional profiling of spermatogenic cell populations on a single-cell …
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Mechanisms driving the expansion and progression of splicing factor-mutant clonal haematopoiesis
… the widespread mis-splicing of a large number of transcripts, although the functional consequences of many of these aberrant splicing events remains unclear. To investigate this, we performed parallel RNA-sequencing and proteomics on SF3B1-K700E cells, characterising the relationship between …
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A molecular investigation of the novel gene underlying autosomal dominant retinitis pigmentosa in a South African family
… to a 10 cM interval on chromosome 17q22. This novel locus, designated RP17, is the eighth identified for adRP. Haplotype construction in the two kindreds, in conjunction with multipoint analyses subsequently fine mapped RP17 to a 1 cM region between microsatellite markers D17S1604 and D17S948. …