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Showing 1 to 20 of 24 for “"nosZ"”.
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Denitrification in a best management practice bioretention system
… bacterial 16S rRNA or a denitrification gene (nosZ) in order to determine presence or absence of denitrifying bacteria. Three dilutions were used to produce semiquantitative results for the abundance of denitrifying bacteria in a sample relative to samples from other depths. The highest numbers …
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Perennial energy crops on riparian buffers affect nitrogen cycling microbial communities
… (nrfA), denitrification (nirK, nirS, norB, and nosZ), and N fixation (nifH). The results indicated that N application significantly affected the abundance of four different functional enzyme groups. The abundance of bacterial amoA (AOB) increased as N fertilization increased, thereby increasing …
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The effect of winter grazing and a nitrification inhibitor on nitrous oxide emissions and denitrification in a stony soil
… in the field, significantly more N₂O reducing nosZ clade-I (P<0.05) and nosZ clade-II (P<0.01) gene copies were found in trampled soil than non-trampled soil. The increase in nosZ gene copies in urine amended trampled soil was accompanied by an increase in the proportion of ¹⁵N labelled urine …
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Mikrobielle Kohlenstoff- und Stickstofftransformationen im Verdauungstrakt von Regenwürmern (Oligochaeta)
… anhand der Analyse von N2O-Reduktasegenen (nosZ) bestimmt. NosZ Sequenzen aus den Verdauungstrakten waren sehr ähnlich zu nosZ Sequenzen aus den umgebenden Böden und verwandt zu unkultivierten Bodenbakterien. N2O und H2 wurden von europäischen Würmern emittiert. Höchste in …
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Greenhouse gas metabolizing prokaryotes in peatlands
… genes narG, nirK/nirS, and nosZ indicated that the diversity of the denitifier community was highest in pH-neutral fen soil and positively correlated with pH. Detected narG affiliated mainly with Betaproteobacterial and Actinobacterial narG. . The number and the identity of …
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Evaluación de comunidades microbianas en suelos semiáridos del sur-oeste bonaerense, bajo distintos sistemas de manejo agrícola
… mayor variación en la secuencia del gen nosZ. Se caracterizaron morfológicamente los aislamientos potencialmente desnitrificadores, los cuales presentaron diferentes modelos de crecimiento. De los cincuenta y siete aislamientos obtenidos, en más de la mitad se confirmó la presencia tanto …
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Soil quality and nitrogen cycling dynamics altered by fertility amendments in intensively managed vegetable production systems
… (nirK and nirS) and nitrous oxide reductase (nosZ) genes, and community structure of bacterial ammonia monooxygenase (amoA) and nirK were quantified in bulk and rhizosphere soil samples collected from open field plots. In 2011, chard yield was lower in the high tunnel than the open field …
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Biochar and pH as Drivers of Greenhouse Gas Production in Denitrification Systems
… Quantify key denitrification genes (nirK, nirS, nosZ) in each treatment combination. Experimental results showed biochar treatment to significantly increase N2O emissions, a result which runs contrary to most, but not all studies regarding its effects on N2O production. Differences between …
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Microbial community analysis for denitrifying biofilters
… Community fingerprint techniques using ARISA and nosZ t-RFLP were also developed to provide high throughput bacterial and denitrifying community data. The spatial structure of microbial communities in a biofilter was characterized using mapping of ecological metrics, MDS plots, and a new …
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The regulation of denitrification in P. denitrificans
… and repressed the expression of nirS, norB and nosZ resulting in detectable nitrous oxide emissions. Therefore, the transcription factors FnrP, NnrR and NarR compete for the binding sites upstream of the denitrification operons in a way that optimizes the metabolic rates of denitrification and …
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Endogenous production and detoxification of a potent cytotoxin, nitric oxide, in Salmonella enterica serovar Typhimurium and Escherichia coli
… archaea and fungi that possess the enzyme NosZ. Furthermore, the contribution of selected NsrR regulon genes, to endogenous N2O production of Salmonella was determined and revealed that HmpA and the Hcp-Hcr operon are both crucial for high N2O levels. These findings provide new insights …
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Mikrobieller Metabolismus von 2,4-Dichlorphenol in der Drilosphäre und Einfluss auf die N2O-Bildung : Verknüpfung von Prozessen mit Populationen
… periplasmatische N2O-Reduktase kodierenden Gens nosZ konnte ebenfalls keinen Effekt von 2,4-DCP auf die phylogenetische Zusammensetzung nosZ-tragender Bakterien nachweisen. Verglichen dazu waren die N2O-Bildungsraten, die Keimzahlen von Denitrifikanten und die Abundanzen relevanter struktureller …
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Evaluation of Passive Reduction of Salts and Nutrients from Greenhouse Effluent using Vegetated Bioreactors
… the abundance of the denitrifying genes nirS and nosZ with the denitrification performance in the T. angustifolia and unplanted units. A greater population of denitrifiers was found in these bioreactors, while the rhizosphere of T. angustifolia displayed the highest microbial activity. The …
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Microbial Regulation of Soil Greenhouse Gas Emissions in a Non-Bloat Legume Grazing System
… abundance. Urine toxicity and increased clade II nosZ transcription likely reduced initial N2O emissions at high urea concentrations, resulting in no difference between cumulative soil N2O fluxes between urea rates and a lower urine-N emission factor for high urea soils. These findings support the …
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Intact soil core experiments reveal that temperature and depth influence microbial community function and impact the fate of nitrogenous fertilizer amendments
… temporal shift in nitrous oxide reductase gene (nosZ) based denitrifier community structure of was evident after 7 days, resulting in denitrifier communities that were significantly distinct. Additionally, qPCR analysis of relevant functional gene transcripts reveals differential trends in gene …
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Evaluation of Digital PCR (dPCR) for the Quantification of Soil Nitrogen Turnover Bacteria in Wetland Mesocosms in Response to Season, Fertilization, and Plant Species Richness
… spp. NOB (AOB, anammox, Nitrospira spp. NOB, and nosZ). The effects of plant species richness were more nuanced, with greater richness significantly impacting the abundance of only a subset of bacterial groups (i.e., the nitrifying bacteria AOB, Nitrospira spp. NOB, and comammox, but not the …
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Performance, denitrification activity, and microbial community dynamics of denitrifying biofilters under fluctuating water level
… fragment length polymorphism (tRFLP) of nosZ, the gene encoding the catalytic subunit of nitrous oxide reduction. In the laboratory biofilter study, the performance was sensitive to changing water level, with 31% nitrate removed at high water level and 59% at low water level. The …
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Gas-phase biofiltration for livestock building ammonia emission mitigation
… to 55%. However, the bacterial communities and nosZ gene communities displayed a functional redundancy to the moisture changes. Interestingly, the real-time qPCR results showed that the quantity of nosZ gene copy was reduced significantly at 63% moisture content. This observation can explain the …
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Microbial community dynamics in denitrifying biofilters receiving agricultural drainage
… Fragment Length Polymorphism (T-RFLP) of nosZ (one of the nitrous oxide reductase genes), and fungal ARISA (FARISA) was used to determine fungal community structure. Spatial and temporal results from the biofilter studies have provided valuable insight into how microbial communities, …
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Mikrobiologie der Stickstoffentfernung in den Biofiltern einer marinen Aquakultur mit geschlossenem Wasserkreislauf
… anhand der funktionellen Markergene narG und nosZ erbrachte eine beträchtliche Anzahl unterschiedlicher Klongruppen. Im Unterschied zum Kultivierungsansatz ergab die vergleichende Analyse der narG-Genbibliotheken sehr klare Unterschiede zwischen den drei untersuchten Teilsystemen. …
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