Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 18 of 18 for “"nonviral"”.
-
Engineering of Nonviral Vectors for Gene Therapy
Finally, for polymeric devices to be made safe and efficient for in vivo usage there must be some means to target them to specific cells or tissues. Various cyclodextrins were conjugated to PEI using an amide linkage. The hydrophobic interior of the cyclodextrin allows the facile addition of small …
-
Application of endostatin using nonviral gene delivery toward the regeneration of articular cartilage
… constructs. The constructs were engineered using nonviral gene therapy, through evaluation of select variables, including regulators (culture media, endostatin plasmid load, method of pEndo lipoplex incorporation, and oxygen tension), scaffold formulation, and cell type. We also investigated …
-
Gene-supplemented collagen-glycosaminoglycan scaffolds for nonviral gene delivery in articular cartilage tissue engineering
… (CG) scaffolds for the nonviral delivery of the gene encoding for insulin-like growth factor (IGF)-I, as a novel means to provide a local, elevated, and prolonged release of a therapeutic growth factor via transfection of cells seeded or migrating within the scaffold. In …
-
Characterization of Tumorigenic Proteins
… 94K T antigen carboxyterminus. The 48K and 55K nonviral antigens, proteins immunoprecipitable by SV40 T antibody from SV40 permissive and transformed cells, were compared to the SV40 large T antigen and to each other by tryptic peptide mapping. The 48K and 55K nonviral antigens are not …
-
New Sensing Modalities for Bacterial and Environmental Phenomena
… response to environmental stimuli via a new nonviral nucleic acid delivery mechanism. This nonviral nucleic acid delivery occurs outside the parameter space of traditional nonviral nucleic acid delivery methods such as electroporation and chemical transformation. The bacteria are immobilized …
-
Poly ([beta]-amino ester)s as pH sensitive biomaterials for microparticulate genetic vaccine delivery
… viral vaccine carriers and the inefficiency of nonviral delivery systems when compared to their viral counterparts. A promising and versatile nonviral delivery method for genetic vaccines involves microencapsulation of antigen-encoding DNA, because such particles protect their payload and target …
-
Imidazole-Containing Polymerized Ionic Liquids for Emerging Applications: From Gene Delivery to Thermoplastic Elastomers
… of charge density and hydrogen bonding on nonviral DNA delivery. Copolymers with higher charge densities exhibited increased cytotoxicity, whereas increased hydroxyl concentrations remained nontoxic. DNA binding affinity increased with increased charge densities and increased hydroxyl …
-
Characterization of Bioreducible Poly(B-amino Ester) Nanoparticles for siRNA Delivery
… to tumor cells for gene knockdown. Presently, nonviral delivery vectors, such as poly(B-amino ester) (PBAE) NP, are beneficial delivery systems because they are less immunogenic and easier to chemically modify than vectors delivered by viruses. Although current formulations of PBAEs allow cargo …
-
Synthesis, characterization, and evaluation of photo-active amphiphiles for gene delivery applications
… to treat a number of diseases. Both viral and nonviral vectors are currently being utilized in gene therapy clinical trials. To overcome the obstacles in the cellular uptake and transfection pathways which impede nonviral gene delivery, novel lipids, polymers, and dendrimers are being …
-
Molecular analysis of La Crosse virus transcription and replication in host cells
… Previous studies demonstrated that certain 5' nonviral sequences predominated during LAC infection of mosquito cells. To characterize this further, the temporal specificity of LAC cap scavenging for transcription of S segment mRNA was analyzed for 42 days after infection of Aedes albopictus …
-
From N to P: Examining Structure-Property Relationships of Ammonium- and Phosphonium-Containing Macromolecules
… the efficacy of phosphonium macromolecules for nonviral nucleic acid delivery was examined. Phosphonium macromolecules more efficiently complexed nucleic acids than ammonium analogs and butyl-containing phosphonium macromolecules delivered nucleic acids more effectively than the ammonium analog. …
-
Optimization of organelle fractionation methods for quantitative analysis of gene delivery trafficking kinetics
Nonviral vector research and development has been stunted by a lack of knowledge and understanding of how vectors are trafficked within the cell. Research currently involves mass screenings of different combinations of vector components without a true understanding of how each component interacts …
-
Strategies for combinatorial development of siRNA conjugate delivery systems
… LNPs are among the most potent and diverse nonviral delivery systems, and they have enjoyed continued improvement in efficacy as new generations of lipids have been explored. This steady progress in lipid performance has relied heavily on studies using combinatorial synthesis and …
-
The Role of Bone Morphogenetic Protein (BMP) Signalling in Lung Cancer and Lung Regeneration
… over-expression vector was generated. Viral and nonviral delivery methods were assessed for their ability to efficiently transfect normal and tumour-derived lung cells. Lipofectamine-2000 was the most efficient non-viral method of gene delivery. This mode of transfection was utilised to transfect …
-
IL -10 gene therapy for the treatment of pulmonary inflammation
… Cationic liposomes represent a class of nonviral vectors that hold great promise as gene delivery vehicles. However, recent studies have shown that they may be associated with some toxicity. We tested the hypothesis that the positive charge of liposomes is a key determinant of toxicity. …
-
Calcium Phosphate Nanoparticle Synthesis and Manufacture using Microwave Processing for Biomedical Applications
… aspect ratios. They were studied as possible nonviral gene delivery carriers. A hydroxyapatite variant with europium doping was also produced and its ability to be used as a fluorescent probe was demonstrated. Plasmid DNA encoding for green fluorescent protein (GFP) was used to confirm the …
-
Tailoring Structure and Function of Imidazole-Containing Block Copolymers for Emerging Applications from Gene Delivery to Electromechanical Devices
… 1-bromobutane provided a potential vector for nonviral gene delivery and polyanion binding. Analysis of DNA and heparin binding using gel electrophoresis revealed a decrease in N/P ratio with increased alkylation percentage. Dynamic light scattering indicated an increase in zeta potential with …
-
Untersuchungen zu bioabbaubaren Genvektoren und in situ gebildeten Filmen als neue Arzneiformen für die Applikation von Gentherapeutika
Die Gentherapie birgt vielversprechendes Potential als Kausaltherapie für die Behandlung zahlreicher vererbter und erworbener Krankheiten. Nukleinsäuren als neue Arzneistoffe sind sehr vielfältig einsetzbar, setzten allerdings einen erfolgreichen Transport zum Wirkort voraus. Lange Zeit dominierten …