Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"nonsense-mediated mRNA decay"”.
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Characterization of nonsense mediated mRNA decay in Schizosaccharomyces pombe
Nonsense mediated mRNA decay (NMD) is a translation-coupled process that preferentially destroys mRNAs harboring premature translation termination codons (PTCs). In mammalian cells, NMD is linked to pre-mRNA splicing. Typically, PTCs elicit strong NMD only if positioned upstream of at least one …
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Physiological role and quantitative aspects of human Nonsense-mediated mRNA decay (NMD)
Nonsense-mediated mRNA decay (NMD) is a molecular pathway of mRNA surveillance that ensures the rapid degradation of mRNAs containing premature translation termination codons in all studied eukaryotes. Originally, NMD was thought of as a quality control pathway that targets non-functional mRNAs …
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Isolation and characterization of Hupf1-containing protein complexes and their role in nonsense-mediated mRNA decay
mRNAs containing premature translation termination codons (PTCs) are usually degraded by the nonsense-mediated mRNA decay (NMD) pathway. The mechanism of NMD in mammals is largely unknown. The cytoplasmic protein Hupf1 was the only trans-acting factor of NMD in mammals that was known at the …
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Tissue-specific classification of alternatively spliced human exons
… conservation, modularity, and susceptibility to nonsense-mediated mRNA decay caused by skipping. Cis-element motifs that might bind protein factors that affect splicing were identified using overrepresentation analysis and conserved occurrence rate between human and mouse.
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Messenger RNA Destabilization by -1 Programmed Ribosomal Frameshifting
… genomic sequences, and suggested a role in mRNA stability. This work improves and extends the computational methods used to search for potential -1 PRF signals. It continues to examine four yeast -1 PRF signals and show that they promote significant mRNA destabilization through the nonsense …
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Expanding the genetic code of a mouse
… insert the unnatural amino acid during mRNA translation, in response to an amber stop codon (UAG) placed at a user-defined site in a gene interest (Davis and Chin, 2012) In this study, I used Acetyllysine(AcK) as unnatural amino acids, N? -acetyl-lysyl-tRNA synthetase (AcKRS) as AcK-tRNA …
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Understanding regulation of mRNA by RNA binding proteins
Posttranscriptional regulation of mRNA by RNA-binding proteins plays key roles in regulating the transcriptome over the course of development, between tissues and in disease states. The specific interactions between mRNA and protein are controlled by the proteins' inherent affinities for different …
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Harnessing transcriptomic data to better understand gene regulatory mechanisms of neurodevelopmental disorders
… gene expression. This thesis investigates the nonsense-mediated mRNA decay (NMD) pathway —a key post-transcriptional mechanism with critical implications for neurodevelopment and NDDs —using transcriptomic data.. To assess the role of NMD, I first create a novel framework which utilises a …
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REGULATION OF EUKARYOTIC GENE EXPRESSION BY mRNA CAP BINDING COMPLEX AND CAPPING MACHINERY
… "cap" to nascent pre-messenger RNA (mRNA) in the nucleus. It is the 5'capping process, which proves vital to creating a mature mRNA. The synthesis of an mRNA followed by its capping is a complex undertaking which requires a set of protein factors. The capped mRNA is then exclusively …
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Molecular visualization and localization of ribosomal subunits interaction in cells
… in the cytoplasm, i.e., transcription and pre-mRNA processing are spatially separated from translation which occurs in the cytoplasm. Ribosome subunits and several translation factors are present in the nucleus but it is understood that they can interact to form the functional ribosome only …
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Long-read transcriptome profiling of germinal centre B cells
… expressed transcripts during antigen-mediated activation of GC B cells in human and mouse. Moreover, identification of individual isoforms with Nexons revealed differential splicing of transcripts, including potentially novel splice variants, as well as changes which were undetectable …
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Identification of novel branch points reveals insights into RNA processing
Pre-mRNA splicing is a ubiquitous process necessary for the production of functional eukaryotic mRNAs. The branch point (BP) sequence is one of three key nucleotide sequences required for pre-mRNA splicing, however, in metazoa it has been less comprehensively studied than the 5' splice site (5'SS) …
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Multiple regulatory layers in the establishment of Rbfox2 splicing networks
Regulated alternative splicing is mediated by RNA binding proteins (RBPs) recognizing short sequence motifs in nascent transcripts. The Rbfox RBPs are highly conserved splicing factors that regulate tissue-specific exon inclusion by binding the RNA motif UGCAUG. We sought to comprehensively define …
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Nonsense-mediated mRNA reduction and pre-mRNA processing in drosophila
… bacteria to mammalian cells, the presence of a nonsense mutation causes a reduction in the level of the mRNA of the corresponding gene. The reduction is not, contrary to initial expectations, due to a passive mechanism by which non translated mRNAs are degraded; rather it is a active process in …
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Tracking translation factors in fission yeast nucleus
… that RPs copurify with TFIIIE in S. cerevisiae. Nonsense-mediated mRNA decay (NMD) removes any mRNA containing premature termination codon (PTC), and requires Upf1. Phospho-Upf1 inhibits conversion of 40S/Met-tRNAiMet/mRNA to translationally competent 80S/Met-tRNAiMet/mRNA initiation complexes to …
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A Novel HIV-1 Genomic RNA Packaging Element and its Role in Interplay between RNAPackaging and Gag-Pol Ribosomal Frameshifting
… RNA takes one of two pathways: 1) to serve as mRNA template for Gag protein translation, or 2) to act as genomic RNA that is packaged into virions. Packaging of two genomic RNAs in the retroviral particle involves interaction between a cis-acting packaging signal and GagNC. Various studies have …
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Cloning and functional characterization of novel genes expressed preferentially in the human retina
… (162/1113), III) no homology to known mRNAs (149/1113), and IV) vector sequences and clones derived from mitochondrial genes (36/1113). After correcting for redundancy, category I represented 234 known human genes and category II a total of 92unknown transcripts. Clones from category I, …