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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"nonlinear microscopy"”.
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Nonlinear Microscopy for Material Characterization
Making use of femtosecond laser sources, nonlinear microscopy provides access to previously unstudied aspects of materials. By probing third order nonlinear optical signals determined by the nonlinear susceptibility chi(3), which is present in all materials, we gain insight not available by …
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Nonlinear microscopy for material characterization
Making use of femtosecond laser sources, nonlinear microscopy exhibits inherent 3- dimensional optical sectioning and provides access to previously unstudied aspects of materials. By probing third order nonlinear optical signals determined by the nonlinear susceptibility (chi-3), which is present …
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Quantitative mRNA detection with advanced nonlinear microscopy
… the diffraction limit. In this thesis, two nonlinear technologies, second harmonic super-resolution microscopy (SHaSM) and transient absorption microscopy (TAM), are developed to detect and quantify single Human edimer receptor 2 (Her2) mRNA transcripts. The SHaSM is used to detect single …
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Adaptive Fiber Source for Label-free Nonlinear Microscopy
Nonlinear microscopy enables label-free visualization of biological processes in live samples at sub-cellular spatial resolution and sub-millimeter penetration depth, enabling the in-vivo study of mechanisms underlying several cellular functions. Due to the low absorption cross-section of the …
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Nonlinear Microscopy for Materials Analysis and Clinical Pathology
… biological systems to characterizing materials, microscopy has facilitated the analysis of micro and nanoscale systems across scientific disciplines. The optical transparency of different biological features allows pathologists to relate what they see on a microscope slide to fundamental …
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Development and applications of high speed and hyperspectral nonlinear microscopy
Nonlinear microscopy refers to a range of laser scanning microscopy techniques that are based on nonlinear optical processes such as two-photon excited fluorescence and second harmonic generation. Nonlinear microscopy techniques are powerful because they enable the visualization of highly …
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Nonlinear Microscopy System and Protocol for Rapid Evaluation of Freshly Excised Human Tissue
… limiting the rapid evaluation applications. Nonlinear microscopy (NLM) is an emerging optical sectioning microscopy technology that enables histological visualization of fresh and intact human tissue without requiring physical sectioning. In this thesis, we developed high-throughput, …
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Rapid evaluation of pathology using nonlinear microscopy with applications in breast cancer, prostate cancer, and renal disease
… required for intraoperative tissue analysis. Nonlinear microscopy (NLM) is a fluorescence microscopy technique that provides subcellular resolution images of tissue using a femtosecond laser to excite fluorescence at the laser focus. Tissue does not need to be microtomed enabling visualization …
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Quantitative characterization of cellular dynamics in wound healing using multimodal microscopy
… multimodal multiphoton and optical coherence microscopy (MPM OCM). Through these studies, additional insights were gained regarding the complex relationships among different skin constituents, and how abnormal pathological states can easily disrupt the homeostasis in the skin microenvironment. …
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Investigation of the ultrafast fiber and hybrid laser systems and their nonlinear wavelength conversion methods in the infrared spectral region /
… middle-infrared laser sources are desirable for nonlinear microscopy and sum-frequency generation spectroscopy. The main goal of this thesis was to create and improve the technologies and methods required for the development of effective and reliable laser sources operating in these spectral …
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Sensorless adaptive optics in advanced microscopy techniques
… mismatch. With a focus on optical trapping and nonlinear microscopy, the combination of a deformable membrane mirror and a random search optimisation algorithm is used to improve system performance at depth by pre-shaping the incoming wavefront with aberrations that are equal but opposite to …
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Nonlinear Optical Techniques For Imaging And Manipulating The Mouse Central Nervous System
… for and demonstration of novel multi-photon microscopy (MPM) and optical manipulation techniques as they apply to studies the mouse central nervous system (CNS), with an emphasis on the spinal cord. The scientific findings which have resulted from the implementation of these techniques are …
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Wide-field Polarimetric Second Harmonic Generation Microscopy for Histology Imaging
Nonlinear optical microscopy provides several contrast mechanisms including second harmonic generation (SHG), third harmonic generation (THG) and multiphoton excitation fluorescence (MPEF) that can contribute with additional information to hematoxylin and eosin (H&E) stained tissue histopathology, …
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Advanced high-power optical parametric oscillators synchronously pumped by ultrafast fibre-based sources
… in fields as diverse as materials processing and nonlinear microscopy / spectroscopy. Two OPOs based on periodically poled lithium niobate (PPLN) and pumped at 1060nm from the first pump source are demonstrated. With 20ps-long pump pulses, average powers of up to 7.3W (3.1W) for the signal (idler) …
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Application of quantitative second-harmonic generation imaging to dynamic systems
… introduces this approach to other quantitative, nonlinear imaging techniques.
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Molecular vibrational diagnostics - from quantum state stability to real time histopathology
… of principle results showcasing the potential of nonlinear interferometric vibrational imaging (NIVI) for cancer diagnosis. NIVI combines chirped-CARS with spectral interferometry to capture background-free vibrational images over a spectral range of 200 cm-1. We show that NIVI acquires Raman-like …
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Early selection of Chinese Hamster Ovary (CHO) cells using simultaneous label-free autofluorescence multi-harmonic (slam) microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) for biopharmaceutical cell line development
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01