Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 140 for “"noncoding"”.
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Genome regulation by long noncoding RNAs
… whether this model might apply to other long noncoding RNAs, many of which are not conserved across species and thus whose functions remain unclear. Through genetic dissection of their local regulatory functions, we show that many of these genomic loci participate in the local regulation of …
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Regulation of Immunoglobulin Gene Diversification By Noncoding RNA's
<p>Small regulatory RNAs supplement the canonical pathways of gene regulation through diverse mechanisms of transcriptional, post-transcriptional, and post-translational silencing. These mechanisms range from "classical" RNA interference (RNAi), to gene repression by microRNAs (miRNAs), to …
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The Regulation and Evolution of long, noncoding RNA Transcription
… leading to the production of thousands of long, noncoding RNAs (lncRNAs, > 200 basepairs), which are distinct from more traditional noncoding RNAs such as transfer RNAs or ribosomal RNAs. This striking finding is observed across all eukaryotes, from humans to budding yeast. It was once thought …
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Comparative transcriptomics of long intergenic noncoding RNAs in Drosophila
… of the amino acid code, long intergenic noncoding RNAs (lincRNAs) can be expected to evolve along different trajectories than protein-coding genes. Most studies of lincRNA evolution analyze evolution only at the sequence level without ascertaining whether the lincRNA is expressed. Over …
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Quantitative Characterization of Conserved Noncoding Regions by Flow Cytometry
… often involves regulatory sequences found in noncoding DNA separate from the gene’s promoter. Conservation of a noncoding region (CNR) across divergent species may indicate a regulatory region critical to basic morphogenesis. CNR-green fluorescent protein (GFP) constructs can demonstrate …
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Quantifying evolution and natural selection in vertebrate noncoding sequence
… molecular evolution and selection that rely on noncoding sequence. Furthermore, our greater understanding of the importance of noncoding DNA demands such methods. This thesis contains the results of the first in-depth genomic-scale analysis using intron substitutions to estimate the neutral rate …
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Small RNAs Regulate Transcription by Interacting with Noncoding RNA Transcripts
… biotinylated RNAs bind to and pull down these noncoding RNAs. Finally, knockdown of the antisense transcript with an antisense oligonucleotide prevent gene activation by duplex RNAs. Following this study, our lab uncovered that duplex RNAs can target beyond the 3' terminus of genes and silence …
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Regulation of Pumilio RNA Binding Proteins by Long Noncoding RNA NORAD
… transcribed and encodes thousands of long noncoding RNAs (lncRNAs). Defining the mechanism of action of lncRNAs has been a critical challenge and priority for understanding their biological functions. An important example of this is the lncRNA NORAD, which is a highly conserved lncRNA that …
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The Modulation of Innate Immunity by Cellular and Viral Noncoding RNAs
Growing evidence demonstrates that endogenous and viral non-coding RNAs possess the ability to alter innate immunity and inflammatory responses. For example, endogenous non-coding RNAs can be recognized by pattern recognition receptors to trigger innate immune activation and inflammation. Many …
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The novel long noncoding RNA lncUSMycN in N-Myc-induced oncogenesis
… study, I experimentally validated the novel long noncoding RNA up-stream of MYCN (lncUSMycN), which was originally annotated by bioinformatics analyses, within the 130 kb amplicon. Using rapid amplification of cDNA ends PCR, 140 extra nucleotides at the 5’-end of lncUSMycN were identified. Gene …
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Caste-Specific Expression Of Coding And Noncoding Genes In Ant Brains
Epigenetics, broadly defined as heritable changes in gene expression independent of changes to the DNA sequence itself, is tightly associated with how one genome can give rise to multiple phenotypes. Social insects are an ideal model system for epigenetics, as their colonies contain individuals …
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MEF2-regulated Gtl2-Dio3 noncoding RNAs in cardiac muscle and disease
… role of selected microRNAs from the Gtl2-Dio3 noncoding RNA locus in the heart. First, I found that Gtl2-Dio3 microRNAs are expressed at higher levels in perinatal hearts compared to adult, suggesting they function in cardiac maturation shortly after birth. I also demonstrated that these …
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Determining the Roles that DICER1 and Noncoding RNAs Play in Endometrial Tumorigenesis
… thesis work I sought to determine the role that noncoding RNAs play in endometrial tumorigenesis. Aberrant methylation of the promoter region of the MLH1 DNA mismatch repair gene in endometrial cancer is associated with loss of MLH1 expression and a "mutator phenotype" in endometrial and other …
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Computational Identification of Noncoding Driver Mutations Based On Impact On Rna Processing
<p>Despite the prevalence of mutations in the noncoding regions of the DNA, their effects on cancer development remain largely uninvestigated. This is especially evident when compared to coding mutations, which have been relatively well-studied and, in certain cases, been identified as driver …
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The mechanism and function of pervasive noncoding transcription in the mammalian genome
… typically leads to short-lived, low-copy number noncoding RNAs (ncRNAs). We are starting to understand the biogenesis and mechanisms regulating the noncoding transcription. However, it is still unclear what's the functional impact of pervasive transcription and the ncRNAs at the level of …
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Functional Analysis of Risk Single Nucleotide Polymorphism and Long Noncoding RNA in Prostate Cancer
… to dysregulate two gene isoforms of a long noncoding RNA (lncRNA) PCAT19. Furthermore, I validated the oncogenic properties of the lncRNA PCAT19, and revealed its function in prostate cancer progression through the upregulation of a subset of cell cycle genes with the RNA binding protein …
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FUNCTIONAL STUDY OF THE NONCODING REGULOME OF THE NUCLEAR FACTOR Y IN HUMAN CANCER
Introduzione. Gli RNA non codificanti (ncRNA) sono implicati nella regolazione di diversi processi biologici, la cui espressione e funzione alterata sono associate a processi di tumorigenesi. In questo lavoro, ci siamo concentrati sugli RNA antisenso (asRNA), che svolgono un ruolo critico nella …
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Bioinformatics approaches to large scale genome comparison, including the identification of conserved noncoding regions
… be exploited in the study of regulatory or other noncoding functional sequences. Using comparative genomics, these noncoding functional sequences can be identified as conserved noncoding sequences (CNSs). Today, the functionality of CNSs is determined by very elaborate and time consuming …
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