Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"non-viral vectors"”.
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Investigation of barriers to non-viral gene delivery and design of novel polymer-based gene delivery systems
… significant challenge facing gene therapy today. Viral vectors remain the dominant approach for addressing the delivery problem; however, concerns regarding the safety of viral vectors have resulted in an increasing interest in non-viral vectors. Non-viral vectors offer the promise of improved …
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Quantitative analysis of non-viral gene therapy in primary liver culture systems
… novel combination therapies for cancers and viral infections, and offer a new and effective platform for next generation vaccines. However, after more than three decades of research and development efforts, clinical success has yet to be realized. Successful delivery of DNA is a crucial first …
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Structure-Property-Transfection Relationships in Polycation-mediated Non-viral DNA Delivery
Non-viral gene delivery agents, such as cationic polyelectrolytes, are attractive replacements to viruses due to the absence of potential immunogenic risk and the ability to tune their macromolecular structure. Although non-viral vectors possess numerous design advantages, several investigators …
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Elucidation of Design Criteria for Non-Viral Gene Delivery Vector Development
… endosomal pathway, dissociation of DNA from its non-viral carrier, migration of DNA to the nucleus, nuclear entry and finally transcription all stand as obstacles to gene delivery. The research presented in this thesis addresses the issue of gene delivery efficiency by studying vector …
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Quantitative analysis and characterization of intracellular gene delivery mechanisms
A goal for gene delivery research is to design vectors capable of (a) delivering transgenes to target cells, (b) yielding efficient gene expression, and (c) minimizing any immune, inflammatory, or cytotoxic response. Current research has focused on developing such vehicles using end gene expression …
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Engineering Lipid Nanoparticle-Mediated Delivery of Nucleic Acids to Lymphoid Organs and Immune Cells
… membranes to reach the cytosol or the nucleus. Viral and non-viral vectors are needed for the protection and effective delivery of nucleic acids. Lipid nanoparticles (LNPs) are an established essential platform for nucleic acid delivery. Efforts have led to the development of vaccines that …
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Investigation of the immunostimulatory activity and vaccine potential of lipid encapsulated plasmid DNA and oligodeoxynucleoties
… and examining their ability to act together as a non-viral DNA vaccine in attempt to address the shortcomings of current DNA vaccine approaches. One focus of this thesis concerns investigating the immunostimulatory activity of LN formulations of CpG-ODN and pDNA. It is shown that despite dramatic …
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Modulation of Dendritic Cells with the Interleukin-10 Gene on Polycation-Modified Polymeric Particles
… An emerging focus in this field is to employ non-viral vectors to deliver immunosuppressive cytokines to dendritic cells (DCs) to attenuate damaging immune responses. DCs serve as potential targets for suppression of T cell responses. In this work, we investigated the ability of …
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Cationic, α-helical polypeptides for cell penetration and non-viral gene delivery
… The development of effective and safe delivery vectors are quite crucial towards gene therapy. Compared with viral vectors, non-viral gene delivery vectors allow the safe delivery of genetic materials with less inherent adverse reactions such as immunogenicity, unexpected viral replication and …
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Synthesis and evaluation of novel non-viral gene delivery systems for prostate cancer targeting
… double than that observed in cells treated with non-targeted dendriplex. The anti-proliferative efficacy of DAB-Lf dendriplex encodingTNFα, TRAIL, or IL-12 was significantly improved compared with unmodified DAB dendriplex. In vivo, intravenous injections of DAB-Lf dendrimer complexed with …
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Design of hybrid retroviral/synthetic gene delivery vectors
… efficient means of delivering genetic material. Viral vectors, for instance, are extremely efficient but potentially pathogenic and immunogenic. They also typically possess a tropism towards specific cellular receptors which is difficult to modify without significant loss in efficiency. Non-viral …
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Preparation and analysis of novel tumour-targeted delivery systems for prostate cancer therapy
… the intravenous treatment of prostate cancer. Non-viral gene delivery is emerging as potential safer alternative to the use of viral vectors for the treatment of various gene related diseases including cancer. Although non-viral vectors may not be as effective as the viral ones, the continuous …
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Gene Therapy and Stem Cell Therapy to Improve the Outcome of Human Islet Transplantation
… loss of islet viability and function caused by non-immune reasons, such as the disruption of islet microvasculature during islet isolation and purification process, hypoxia in the core of islet grafts and production of inflammatory cytokines at the transplantation sites.</p> <p>Several …
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Gene Delivery to the Blood-Brain Barrier for Ischemic Stroke Therapy
<p>Stroke is a major cause of death in the United States. We aim to develop safe and efficient approaches for the treatment of stroke. Regenerative therapies have been identified as the major need for curing stroke [1]. We explored two different carriers (poly (ethylene glycol)<sub>5k</sub>-poly …