Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"non-viral gene delivery"”.
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Elucidation of Design Criteria for Non-Viral Gene Delivery Vector Development
… endosomal pathway, dissociation of DNA from its non-viral carrier, migration of DNA to the nucleus, nuclear entry and finally transcription all stand as obstacles to gene delivery. The research presented in this thesis addresses the issue of gene delivery efficiency by studying vector …
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Cationic, α-helical polypeptides for cell penetration and non-viral gene delivery
Gene therapy is showing great potentials in treating various kinds of genetic diseases. The development of effective and safe delivery vectors are quite crucial towards gene therapy. Compared with viral vectors, non-viral gene delivery vectors allow the safe delivery of genetic materials with less …
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Photo-responsive degradable poly (beta-amino ester) toward non-viral gene delivery
… ester) (PBAE) is one of ideal candidates for non-viral gene therapy due to its desired gene delivery efficiency, biocompatibility, and biodegradability. In order to further improve the gene delivery efficiency of PBAE by facilitating the intracellular DNA dissociation, we herein report the …
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Gene-supplemented collagen scaffolds for non-viral gene delivery for brain tissue engineering
… vehicle with therapeutic molecules, cells and/or genes has yielded promising results for brain injury repair. The purpose of this thesis was to develop a collagen scaffold for the non-viral delivery of the gene encoding for Glial Cell-Derived Neurotrophic Factor (GDNF); hence to provide a local, …
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Synthesis and evaluation of novel non-viral gene delivery systems for prostate cancer targeting
… disease. Among novel experimental strategies, gene therapy holds great promise for the treatment ofcancer, but its use is currently limited by the lack of delivery systems able to selectively deliver the therapeutic genes to the tumours after intravenous administration without major drawbacks. …
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Effect of uptake pathway on non-viral gene delivery in vitro and in vivo
Efficient non-viral gene delivery often involves the conjugation of a cell-specific ligand to the vector, which directs the vector to its intended target through binding to a cellular receptor followed by internalization via endocytosis. However, little is known in terms of how the various …
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Non-covalent Intermolecular Interactions in Polymer Design: Segmented Copolymers to Non-viral Gene Delivery Vectors
Non-covalent intermolecular interactions play a large role in determining the properties of a given system, from segmented copolymers to interactions of functionalized polymers with non-viral nucleic acids delivery vehicles. The ability to control the intermolecular interactions of a given system …
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Regulated release of a novel non-viral gene delivery vector from electrospun coaxial fiber mesh scaffolds
… polymers were fabricated into a novel gene delivery agent as well as three dimensional scaffolds that act as reservoirs and controlled release constructs. To fabricate a novel gene delivery agent a commercially available cationic polymer, poly(ethylenimine), PEI, was chemically …
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Investigation of barriers to non-viral gene delivery and design of novel polymer-based gene delivery systems
The safe and effective delivery of therapeutic genes is the most significant challenge facing gene therapy today. Viral vectors remain the dominant approach for addressing the delivery problem; however, concerns regarding the safety of viral vectors have resulted in an increasing interest in …
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Engineering Matrix Properties for Stem Cell Culture and Gene Delivery
Tissue engineering aims to regenerate lost or damaged tissues and frequently combines therapeutic cells, biomaterial scaffolds, and therapeutic molecules in efforts to do so. One of the primary considerations in such therapies is to orchestrate the interactions between these components to regulate …
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Structure-Property-Transfection Relationships in Polycation-mediated Non-viral DNA Delivery
Non-viral gene delivery agents, such as cationic polyelectrolytes, are attractive replacements to viruses due to the absence of potential immunogenic risk and the ability to tune their macromolecular structure. Although non-viral vectors possess numerous design advantages, several investigators …
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Reconfiguring the side-chain functionality of cationic helical polypeptides toward maximized gene delivery capabilities
The rational design of effective and safe non-viral gene vectors is largely dependent on the understanding of the structure-property relationship. This thesis aims to report the design of a new series of cationic, α-helical polypeptides with different side charged groups (amine and guanidine) and …
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Nanostructured gene and drug delivery systems based on molecular self-assembly
… supramolecular structures governed by weak, non-covalent interactions. In recent years, molecular self-assembly has been used extensively as a means of creating materials and devices with well-controlled, nanometer-scale architectural features. In this thesis, molecular self-assembly is used …
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Enhanced polymeric nanoparticles for gene delivery
The potential of gene therapy to treat disease and improve human health is tremendous. The failure of viral gene therapy clinical trials due to toxicity, immunogenicity, and carcinogenicity has been tragic and strongly motivates a non-viral approach. However, non-viral gene delivery is currently …
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Bioreducible Polymer Nanosystems For Gene Delivery
… the research of bioreducible polymers for gene delivery. A series of bioreducible poly(amido amine)s (PAAs) were synthesized. They complex with DNA forming polyplex nanoparticles and layer-by-layer (LbL) thin films as gene delivery vectors. Atomic force microscope (AFM), especially in situ …
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Novel non-viral delivery systems for cancer gene therapy
The non-viral gene delivery systems dendrimers have been widely studied for cancer therapy. However, due to lack of targeting efficacy, their therapeutic applications are still limited. In this present study, our aim was to investigate the efficacy of four formulations of modified generation- 3- …
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Investigation of the immunostimulatory activity and vaccine potential of lipid encapsulated plasmid DNA and oligodeoxynucleoties
DNA vaccines offer unique promise as a means of generating immunity against infectious and malignant disease. Unfortunately a number of obstacles, including rapid degradation of naked plasmid DNA (pDNA), poor cellular uptake by antigen presenting cells (APCs) and subsequent low levels of gene …
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Design of folic acid, transferrin, and integrin receptor specific polyamidoamine dendrimers for gene delivery
Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-06-14T15:37:18Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Hwang_Mark.doc: 55420928 bytes, checksum: 0010b064d38a25a15d06deb51af67ca4 (MD5) Hwang_Mark.pdf: 22852276 bytes, checksum: …
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Multifunctional bioreducible nanoparticles for gene therapy
<p>Gene therapy is a promising therapeutic strategy to treat diseases caused by single or multiple gene mutations. Non-viral gene delivery vectors exhibit many advantages over viral vectors, including enhanced safety, versatility in choosing different types of therapeutic nucleic acids, and ease of …
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