Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"non-transgenic"”.
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Exploring Non-transgenic CRISPR-Cas9 gRNA Delivery Using Transactivation in Nicotiana benthamiana
… or deletions that render the target gene non-functional. It is also possible to mutate the Cas9 protein so that it loses its cutting function, but can still sit on the target DNA when in a complex with gRNA. This deactivated Cas9 (dCas9) can be fused with transcriptional activators or …
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Effects of simulated drift of glyphosate, imazethapyr, glufosinate, and imazamox to non-transgenic rice
Four studies were conducted at the LSU AgCenter Rice Research Station near Crowley, Louisiana to evaluate the effects of simulated herbicide drift on ‘Cocodrie’ rice. Herbicides were applied at 6.3 and 12.5% of the labeled usage rate of 863 g ae/ha of glyphosate, 70 g ai/ha of imazethapyr, 493 g …
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Characterization of the Dunkin Hartley guinea pig as a non-transgenic and multimorbid model of brain aging
Alzheimer's Disease and Alzheimer's Disease Related Dementia (AD/ADRD) affect an estimated 55 million people worldwide; a staggering figure that is expected to grow in the coming years. With this projection looming, we have yet to identify any effective cures, treatments, or preventative …
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Evaluation of risks associated with close environmental contact to swine that are transgenic for a bovine milk protein alpha-lactalbumin
Assessment of the risks associated with exposing non-transgenic animal populations to transgenic animals is important to the future contributions of transgenic livestock to society. Evaluation of the potential for transfer of a transgene (Tg) from livestock to a non-transgenic animal during …
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Genetically Modifying Mosquitoes Without Compromising Fitness: Can It Be Done?
… in mosquitoes. We previously generated a transgenic Anopheles stephensi line with increased insulin signaling in the fat body. Surprisingly, these transgenic mosquitoes survived significantly longer than their non-transgenic siblings, while in nearly every other organism increased IIS …
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Generation and analysis of transgenetic mice expressing catalytically inactive recombination activating gene-1
… To test this hypothesis we have generated transgenic mice overexpressing a catalytically deficient form of RAG1 in which the three residues comprising the DDE motif have been mutated to alanine. We show here that transgenic mice, but not non- transgenic littermates, accumulate an unusual …
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Thiarubrine production in roots and root cultures of Ambrosia chamissonis
… were identified from roots and root cultures. Transgenic and non-transgenic root cultures were generated and shown to produce the same compounds as occur in natural roots. Quantitative chemical profiles of the cultured roots matched those of roots which had little secondary growth. Elicitation, …
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LRRK2 Regulates Actin Dynamics in ADP-Treated Microglia
… activation and the movement of microglia than non-transgenic LRRK2 did. Furthermore, microglial migration defects were also observed in stab-wounded LRRK2 G2019S TG mice brain. Taken together, these results suggest that LRRK2 suppresses microglial movement by inhibiting FAK activation. …
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Development and use of a synthetic biology toolkit to enhance high value lipid production in Nannochloropsis oculata
… NR. This is believed to be the first report of non-transgenic HDR-mediated gene editing in this Nannochloropsis species. HDR gene editing using the BEC10 system did not yield any mutant strains, however. Further development of BEC10 is required to address the timing of BEC10 expression and tools …
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Development of genome editing in potato and tobacco
… CRISPR/Cas9, can be delivered using conventional transgenic methods, or non-transgenic methods, such as transfection of CRISPR/Cas9 ribonucleoproteins (RNPs) in protoplasts followed by plant regeneration (Chen et al., 2019). The non-transgenic approach is preferred in many cases as it avoids the …
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Effects of Proton Radiation on Behavior in a Mouse Model of Alzheimer’s Disease
… To test this hypothesis, we exposed young double transgenic APP/PSEN1 mice (a commercially available strain engineered to develop AD-like neuropathology) and their wild-type (non-transgenic) counterparts to low doses of 150 MeV proton particle radiation and assessed the effects on …
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Transgenic manipulation of polyamine metabolism in poplar
… under the control of 2X 35S CaMV promoter. The transgenic cells (line 2E) showed elevated levels of mouse ornithine decarboxylase enzyme activity, several fold higher amounts of putrescine, a small increase in spermidine, and a small reduction in spermine as compared with non-transgenic (NT) …
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Effect of Ploidy Elevation, Copy Number and Parent-Of-Origin on Transgene Expression in Potato
… seed germination was observed for several transgenic lines in an in vitro study. Ploidy elevation did not affect a highly expressed single copy cry3Aa transgene, simplex or duplex, transmitted through pollen to 4x-2x hybrids. By contrast, multiple copies of cry3Aa triggered significant …
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Small RNAs in Tomato: from defence to development
… discovered tomato TAS5 locus. I also generated transgenic tomatoes constitutively expressing target mimic RNAs that sequester different miR482/2118 members. These tomato mimic RNA lines were less susceptible than their non-transgenic precursors to pathogens Phytophthora infestans and Pseudomonas …
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Antisense Reduction of the Protein Tau Attenuates Neuronal Hyperexcitability and Permits Clearance of Intraneuronal Tau Accumulations in vivo
… to assess the roles of endogenous tau, non-transgenic mice do not develop tau aggregates. One of the main pathological AD hallmarks is the presence of tau inclusions, so to better test the effect of tau reduction on pathological tau species, we reduced human tau in a transgenic tauopathy …
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Role of Stat3 In Keratinocyte Stem Cells During Skin Tumorigenesis
… role of KSCs in skin tumorigenesis, K5.Stat3C transgenic (Tg) mice which express a constitutively active/dimerized form of Stat3 called Stat3C via the bovine keratin 5 (K5) promoter were studied. The number of CD34 and α6 integrin positive cells was significantly reduced in Tg mice as compared …
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Monocytes as Gene Therapy Vectors for the Treatment of Alzheimer’s Disease
… cells are actively recruited to the brains in AD transgenic mouse models compared to non transgenic mice. As an approach to increase expression of NEP in a transgenic mouse model of AD, we developed an ex vivo gene therapy method utilizing bone marrow monocytes from GFP mice. These monocytes were …
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Developing Hybrid Cotton (Gossypium spp.) Using Honey Bees as Pollinators and Roundup Ready® Gene as Selection Trait
… years (2005-2007) in Louisiana. Crosses between non-transgenic and transgenic varieties were made in 2005 to obtain F1 cottonseeds using honey bees. In 2006, F2 cottonseed was obtained. In 2007, F1, F2, and parents were field tested using a randomized complete block design with 3 replications in …
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Peptide immunotherapy in models of allergic airways disease
… inflammation (AAI). Trackable, T cell receptor transgenic T cells recognizing the immunodominant 323-339 peptide of OVA (pOVA) allow mechanistic investigation of PIT in response to pOVA. This thesis investigated the hypothesis that strong, systemic T cell responses induced by intravenous …
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Expanding Genetic and Genomic Resources for Sex Separation and Mosquito Control Strategies
… marker lines. We show that by linking a transgenic marker to the male-determining locus (M locus), or by linking the male-determining Nix gene to a marker, males can be precisely separated from females. We also produce a two-marker transgenic line that allows for both non-transgenic male …
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