Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"non-ribosomal peptide synthetase"”.
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A functional genomics approach: Characterisation of two Non-ribosomal peptide synthetase genes, and investigation of an adaptive response towards alkylating DNA damage in Aspergillus fumigatus.
… encode virulence factors, or novel drug targets. Nonribosomal peptide (NRP) synthetases have been implicated in virulence of A. fumigatus and other fungi. The work presented here reports opposing roles for two previously uncharacterised NRP synthetases with respect to A. fumigatus virulence. The …
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Untersuchungen auf Sekundärstoffproduktion und physiologische Charakterisierung von marinen heterotrophen Bakterien aus dem deutschen Wattenmeer
… for polyketide synthase-, FADH2-halogenase- and non-ribosomal peptide synthetase genes. Additionally agar diffusion assays were applied to investigate the antagonistic activity of the strains, and some bacteria were tested for acylated homoserine lactones (AHL) production. Three isolates were …
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Genomics and transcriptomics of the industrial acarbose producer Actinoplanes sp. SE50/110
… biosynthetic gene cluster sequence, several new non-ribosomal peptide synthetase-, polyketide synthase and hybrid-clusters were identified on the Actinoplanes genome. Another key finding represents the discovery of a functional actinomycete integrative and conjugative element, which might pose an …
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Studies on the biosynthesis of oudenone and verucopeptin
The biosynthesis of the fungal metabolite oudenone ( 67 ) was investigated. The Ì-diketone ( 70 ) was synthesized and shown to be the open-chain biosynthetic precursor of oudenone. Intact incorporation of 70 into 67 was achieved upon incubation of 2 H-labeled, N -acetylcysteamine thioester …
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Characterizing and Engineering Thioesterases as Biocatalysts for Macrocyclization
… here describes the characterization of two non-ribosomal peptide synthetase (NRPS) TE domains and their use as biocatalysts for the chemoenzymatic synthesis of two cyclic depsipeptide NP. Unlike other characterized NRPS TEs, these two demonstrated the ability to catalyze macrolactonization …
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Functional Genomic Analysis of Natural Product Biosynthesis and Secretion in Aspergillus fumigatus
… A. fumigatus produces metabolites, through a non-ribosomal peptide synthesis mechanism, including iron-chelating molecules known as siderophores and other unusual compounds such as gliotoxin. These provide the fungus with a unique strategy for survival in the host, and contribute to …
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Functional Characterisation of the Eukaryotic Translation Elongation Factor 1Bγ in Aspergillus fumigatus; A Proteomic Systems Approach
… comparative proteomics of A. fumigatus Δpes3, a non-ribosomal peptide synthetase deficient strain, supports a structural role for this peptide with the altered expression of proteins functioning in morphogenesis and germination. A. fumigatus Δpes3 exhibited a reduced germination rate in static …
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Activación de rutas de biosíntesis silenciosas en Streptomyces albus J1074
… un sistema PKS-NRPS (polyketide synthase-non-ribosomal peptide synthetase) y tres conteniendo genes que codifican NRPSs, por su potencial biosintético de acuerdo a las predicciones bioinformáticas obtenidas con los programas Antismash y PRISM. A continuación, se llevó a cabo la …
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The role of genomic islands in virulence of Pectobacterium carotovorum subspecies brasiliensis on potatoes
… disease of potatoes were compared to those of non-blackleg causing strains, to identify GIs in blackleg causing strains and novel virulence factors encoded on these islands. First, the identity of a highly aggressive P. carotovorum subsp. carotovorum strain ICMP19477 and other New Zealand …
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Identification and preliminary characterization of the 2,5-diphenyloxazole biosynthetic pathway in streptomyces polyantibioticus SPRT
… of this molecule was proposed, whereby a non-ribosomal peptide synthetase (NRPS) condenses a molecule of benzoic acid with 3-hydroxyphenylalanine. The dipeptide is converted to a diphenyloxazole derivative by heterocyclization and a final decarboxylation step leads to DPO. To determine …
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Investigating and engineering the post-translational modifications in RiPP natural product biosynthesis (I) Thiopeptides (II) Sactipeptides
… are derived from polyketide synthase (PKS) or non-ribosomal peptide synthetase (NRPS) families, including well-known antibiotics such as the beta-lactams, tetracyclines, macrolides, and glycopeptides. With the rising resistance to these proven antibiotic classes, alternative sources of …
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Investigation of a novel iron-uptake system and other genomic features in mecC Staphylococcus aureus
… of a novel locus encoding a siderophore-like non-ribosomal peptide synthetase (NRPS), directly downstream of the SCCmec insertion site in mecC S. aureus isolates. A homologous region was identified in Streptococcus equi 4047 (S. equi) which encodes a NRPS termed ‘equibactin’ that is involved …
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Screening environmental actinobacteria for antimycobacterial antibiotics and characterisation of Kribbella stellenboschensis sp. nov
… for seven isolates to produce ansamycin, glycopeptide, non-ribosomal peptide, and/or TypeII polyketide antibiotics was determined by detection of antibiotic biosynthetic gene clusters using PCR. Strain M27 demonstrated the potential to produce all the aforementioned antibiotics. Strain Y10 …
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A genomics-led approach to deciphering heterocyclic natural product biosynthesis
… trienes produced by a polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) pathway in Streptomyces sp. KO-7888 and Streptomyces sp. OM-5714. The phthoxazolin pathway was used as a model to study 5-oxazole and primary amide formation in PKS-NRPS pathways. An unusually large gene …