Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"non-muscle myosin"”.
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THE ROLE OF NON-MUSCLE MYOSIN IN TENDON AND ATTACHMENT UNIT FORMATION AND MATURATION
Mechanical force via muscle contraction is critical for the development and maturation of tendons and their integration with the adjacent bone through a specialized tissue known as an attachment unit. The mechanisms by which applied loads regulate tendon and attachment unit biology are not well …
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Molecular and microscopical analysis of pathogenic streptococci - studies on surface proteins interacting with human cells and extracellular matrix
… dependent manner and we were able to identify non-muscle myosin IIA as a potential cellular ligand for Slr. We could further confirm the presence of non-muscle myosin IIA in HaCat cell lysate and that Slr binds to non-muscle myosin IIA. In summary, we have characterized a novel adhesin of S. …
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Adherens Junctions and The Actomyosin Network Regulate Organ Growth By Modulating Hippo Pathway Activity In Drosophila
… shows a cell autonomous decrease and a cell non-autonomous increase of Hippo reporter activity. We provided several lines of evidence to show the differential regulation in polarity protein localizations and oncogenic cooperative overgrowth by AJs and basolateral complexes. Finally, we show …
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Mechanotransduction at the nuclear envelope: the role of forces in facilitating embryonic stem cell fate decisions
… the cytoskeleton – notably the molecular motor non-muscle myosin II - and key structural and chromatin binding proteins in the nuclear envelope, notably, the lamins (LMNA), Lamin B receptor (LBR) and components of the LINC complex (nesprins/KASH). The structural proteins in the nuclear envelope …
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The Role of UNC-45 in Heart Development and Human Breast Cancer
… as a molecular chaperone of body-wall muscle myosins. In human and other vertebrates, two UNC-45 genes code two UNC-45 protein isoforms: UNC-45A/a and UNC-45B/b. UNC-45A/a is expressed in all cell types, whereas UNC-45B/b only exists in heart and skeletal muscles. This thesis …
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Spatiotemporal organization, regulation and function of traction during neutrophil chemotaxis
… of tractions depend upon the activity of non-muscle myosin IIA. Specifically, traction development at the leading edge requires myosin light chain kinase (MLCK)-mediated myosin II contractility and is necessary for α5β1-integrin activation and leading edge adhesion. Localized myosin II …
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Taking shape : the path to myosin activation in the Drosophila ventral furrow
… the correct shapes. Force generation by actomyosin networks is a major driver of morphogenesis across many systems. The organization of actomyosin networks across multiple length scales is critical in generating biological form, including the Drosophila melanogaster ventral furrow. Using …
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Anillin Stabilizes Membrane-cytoskeleton Interactions During Drosophila Male Germ Cell Cytokinesis
… segregation. Anillin binds filamentous F-actin, non-muscle myosin II and septins, and in cell culture models has been shown to restrict actomyosin contractility to the cleavage furrow. Whether anillin also serves this function during the incomplete cytokinesis that occurs in developing germ cells …
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Developmental regulation of smooth muscle contraction in the urinary bladder
Smooth muscle cells in the urinary bladder are involved in the control of urine filling and emptying. We have used mice to study the contractile regulation of urinary bladder smooth muscle during the fetal period. We found that nerve-induced contractions are mainly dependent on muscarinic receptor …
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Destabilization of IL-8 mRNA by Anthrax Lethal Toxin: Demonstration of the Requirement for TTP and Examination of its Cellular Interactions
… function. Identified TTP-associated proteins, non-muscle myosin heavy chain 9 (myosin-9) and HSC-70, were examined for their role in IL-8 transcript decay. Knock-down of each protein led to a slower rate of IL-8 mRNA destabilization. However, treatment of LeTx continued to mediate accelerated …
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Matrix Stiffness Regulates Glial Cell Morphology and Differentiation
… our laboratory have shown that inhibition of non-muscle myosin II (NMII) activity has opposite effects on the formation of myelin by oligodendrocytes (OL), the myelinating glia of the central nervous system (CNS) and Schwann cells (SC), which perform the same function in the peripheral nervous …
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Conformational Flexibility in Protein Function: Dynamics of S100A4, Photosynthetic Reaction Centre and the Prenylating Enzymes UbiA and MenA
… structure of human S100A4 in complex with a non-muscle myosin IIA (NMIIA) fragment was solved to 1.9Å and contributed to our understanding in the structural mechanism of S100A4 mediated filament assembly which is believed to promote metastasis. The X-ray structure shows that the binding …
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Investigating Transluminal Endothelial Bridging in a Microvessel-on-a-Chip
… significantly higher density near the edge of non-healing wounds. Next, I undertook multipoint 3D timelapse microscopy of the microphysiological system which revealed the evolution of circumferentially orientated endothelial cell protrusions into transluminal bridges forming by partial …
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Mechanical Regulation of Angiogenesis in a Biomimetic Model
… for successful multicellular invasion. While actomyosin contractility is well studied in single cells on flat surfaces, less is understood about its regulation of collective cell migration, including during angiogenesis whereby endothelial cells from existing vessels invade as multicellular …
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Epidermal growth factor receptor signalling regulates ommatidial rotation during Drosophila eye development
… mutants. Genetic interactions further implicate non-muscle Myosin II as well as genes involved in actin polymerisation/depolymerisation in this process. This study also describes the design of an F1 screen intended to identify new genes involved in ommatidial rotation. Preliminary results …
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Phenotypic Analysis of Platelets in Patients with Platelet Function Disorders
… evaluated for aberrant persistence of platelet non-muscle myosin IIB (MYH10) in a cohort with PFD, including those caused by mutations in transcription factors, such as RUNX1. While some participants with RUNX1 mutations showed the expected abnormal MYH10 findings, one had consistently normal …
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Development of a Generalizable Assay for Probing the Effects of Mechanical Force on the Function of Fluorescent Proteins within Molecular Tension Sensors
… individual focal adhesions (FAs) in an actomyosin-dependent manner, and that both load magnitude and load duration likely play a role. Additionally, we found that this force-mediated loss of FP function, or “mechanical quenching,” is a reversible process, consistent with nonequilibrium …
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Dynamins and myosin-II regulate the distinct modes of synaptic vesicle exocytosis in mature cerebrocortical nerve terminals and this involves calcium dependent phosphorylations.
… involving blockade of the ATPase activity of non-muscle myosin-II suggest that myosin-II may also be able to regulate the fusion pore, independent of dynamin-I, when a different stimulus (HK5C) is employed. The three stimuli employed here produced distinct kinetics for changes in [Ca2+]i and …
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INTERRELATIONSHIP BETWEEN CONTRACTILITY AND DYNAMICS OF ACTOMYOSIN SYSTEM IN NON-MUSCLE CELLS
Contractile actomyosin systems in non-muscle cells are essential for a variety of cellular processes including adhesion and morphogenesis. However, how actin dynamics (in particular, incorporation-dissociation driven flow along the fibers) and myosin dependent contractility are related to each …
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The role of actomyosin contractility in zebrafish neural tube formation through hollowing.
… hollowing lumen formation. While the role of actomyosin contractility is well understood in primary neurulation, for example through constriction of apical-lateral junctions to mediate neural plate bending, its role in secondary neurulation is less clear. In this thesis, I investigated the role of …