Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 88 for “"non-homologous end joining"”.
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Determining molecular mechanisms of DNA Non-Homologous End Joining proteins
… 5' hydroxyl groups and abasic sites. Non-homologous end joining (NHEJ) is a major pathway responsible for the repair of these complex breaks. Proteins involved in NHEJ include the Ku 70/80 heterodimer, DNA-PKcs, processing proteins including Artemis and DNA polymerases µ and λ, XRCC4, …
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Understanding The Roles of Non-Homologous End Joining and P53 After Dna Damage
… One of the major DNA repair pathway that mends DNA double strand breaks is non-homologous end joining (NHEJ). Abrogating the NHEJ pathway leads to an accumulation of DNA damage in the lymphoid system that triggers p53-mediated apoptosis; complete deletion of p53 in this system leads to …
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Investigating the regulation of DNA non-homologous end-joining through Ku70/80 interacting factors
… repair pathways for double-strand breaks exist: homologous recombination (HR) and non-homologous end-joining (NHEJ). Whilst the regulation of HR has been heavily investigated, the regulation of NHEJ remains to be fully explored. The aim of this thesis is to investigate the regulation of DNA NHEJ …
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Altered Kinetics of Non-Homologous End Joining Mediated DNA Repair in Mouse Models of Aging and Leukemia
… The majority of these DSB are repaired by Non-homologous end joining (NHEJ). Unrepaired DSB can lead to genomic instability resulting in cell cycle arrest, apoptosis, and mutations. Thus, delineating this DNA repair process is important in understanding the molecular mechanisms of aging and …
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An exploration of the interplay between HSV-1 and the non-homologous end joining proteins PAXX and DNA-PKcs
… but most remain uncharacterised in this respect. Non-homologous end joining (NHEJ) is a mechanism of double stranded DNA (dsDNA) repair that functions to rapidly mend broken DNA ends. The NHEJ machinery is well characterised in the context of DDR but recent studies have linked the same proteins to …
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Characterization of a Novel 53BP1-Dependent Mechanism that Promotes Non-Homologous End Joining of Deprotected Telomeres by Increasing Chromatin Mobility
… for some of these factors in DSB repair through non-homologous end-joining (NHEJ), the mechanism of their contribution has not been established. To study the regulation of NHEJ, we have used as a model system dysfunctional telomeres, which are uncapped by the removal of the shelterin component, …
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Identification and Investigation of Novel DNA Damage Repair Genes Via Network Analysis
… General DNA damage repair mechanisms involve homologous recombination (HR), or non-homologous end joining (NHEJ). Ongoing identification of new players in DSB repair leads us to believe there are more undiscovered genes in this pathway. The highly complex and conserved nature of DSB repair …
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Influenza Viral Protein Interaction Detecting Methods Development and A.M1-host Interplay Network Investigation
<p>The viral RNA-dependent RNA polymerases of influenza A and B are trimetric complexes (PA, PB1, and PB2 subunits), and are crucial for both transcription and replication of the viral genome. While there are extensive data on the structure and function of influenza A polymerase, the influenza B …
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ANALYSIS OF B LYMPHOCYTES IN MOUSE MODEL LIGASE IV WITH HYPOMORPHIC MUTATION IN VDJ RECOMBINATION ASSOCIATED WITH GROWTH DEFECT
… doublestrand breaks (DSBs) in mammalian cells is non-homologous end-joining (NHEJ), a process that involves the DNA-dependent protein kinase , XRCC4 and DNA ligase IV. Rodent cells and mice defective in these components are radiation-sensitive and defective in V(D)J-recombination, showing that …
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The Rad51 family of proteins: Interactions, vitamin D, and implications in head and neck cancer
… (DSBs) is accomplished through the pathways of non-homologous end-joining (NHEJ) or homologous recombinational repair. Five human proteins with homology to Rad51 known as the Rad51 paralogs, Rad51B, Rad51C, Rad51D, XRCC2, and XRCC3, whose loss of function in cell lines leads to high chromosomal …
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Application of CRISPR/Cas9 to edit genes affecting seed morphology traits in wheat
… The DSB are repaired through the error-prone non-homologous end joining process causing insertions or deletions that may result in loss-of-function mutations. Here, we have developed an effective wheat genome editing pipeline. We used next-generation sequencing (NGS) data to estimate genome …
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The circadian Control of DNA end Resection
… stand as the most cytotoxic lesion the cell can endure, they have evolved two major pathways to counter these: the religation of the broken ends (Non-Homologous End-Joining, NHEJ) and the repair of the lesion using a homologue sequence (Homology-Directed Repair, HDR). The choice between these two …
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Genomic nucleic acid memory storage with directed endonucleases
… cleave viral DNA using an RNA-guided directed endonuclease. Cuts left by the endonuclease are repaired by an error-prone DNA damage repair mechanism, namely non-homologous end joining (NHEJ), likely to leave mutations at the cut sites. Defining the cut site with pathway-dependent transcription …
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The role of linker histone variant in double strand break repair
… strand break (DSB) repair pathway known as non-homologous end joining (NHEJ). NHEJ, in vivo, requires the KU complex, the DNA dependent protein kinase (DNA-PK) and the DNA ligase IV/XRCC4 (LX) complex. In vitro analysis has shown that several linker histone variants are all phosphorylated by …
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Discovering and Characterizing New Homing Endonucleases for Genome Engineering
LAGLIDADG Homing Endonucleases (LHEs) are a family of highly specific DNA-cutting enzymes capable of recognizing target sequences of ~20 bp. In many eukaryotes, including humans and yeast, double-strand breaks induced by LHEs stimulate repair by Homologous Recombination, which can be used to alter …
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Investigation of The Roles of Asf1 and Caf-1-Mediated Chromatin Assembly In The Human Dna Damage Response
… hinders loading of Rad51 onto ssDNA during homologous recombination, as a consequence of reduced recruitment of the Rad51 loader MMS22L/TONSL to ssDNA, resulting in persistent RPA foci, extensive DNA end-resection, and persistent activation of the ATR-Chk1 pathway. By contrast, ASF1 and …
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Modulation of DNA repair pathway after CRISPR/Cas9 mediated Double Stranded Break
… system. The DSB is repaired by either non-homologous end joining (NHEJ) or homologous recombination (HR), and the repair is commonly processed through NHEJ because it is the dominant repair pathway in most cell types. The goal of this study is to modulate DNA repair system of somatic …
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Crosstalk between DNA end resection and RNA metabolism
… suffers thousands of lesions from exogenous and endogenous sources. Nevertheless, double-strand breaks (DSBs) are the most dangerous form of DNA damage because if they are not properly repaired they can lead to mutations and genomic instability. In order to repair them, cells have developed two …
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Genetic and epigenetic regulation of the cell wall glycoproteins, Flo10p and Flo11p, generate phenotypic variation in S. cerevisiae
… Genetic analysis demonstrates that both homologous recombination (HR) and non-homologous end-joining (NHEJ) pathways of DSB repair maintain genetic stability at the IRA loci. Since these pathways specifically process a DSB substrate, this suggests that directed DSB formation may be the …
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The Role of ZMYND8 in Immunoglobulin Class Switch Recombination
… through the DNA Damage Repair (DDR) pathway, non-homologous end-joining (NHEJ). Critical components of CSR are 53BP1 and its effector RIF1 because they inhibit end resection to promote NHEJ and oppose competing pathways in DDR. However, the mechanism by which RIF1 effects end-protection in CSR …
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