Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"non-coding DNA"”.
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Dewdrops on the genome : regulation of gene expression by biomolecular phase separation
… due to the selective expression of particular coding DNA-elements (genes) into RNA. Mutations or dysregulation in control of gene expression underlie many diseased states, including cancer and neurodegenerative disorders. Non-coding DNA elements called enhancers orchestrate the complex …
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Interactions Between Platinum-Intercalator Hybrid-Agents and The Human Telomeric G-Quadruplex
Telomeres are stretches of non-coding DNA sequences in complex with multifunctional proteins, which form the termini of the chromosomes in most eukaryotic organisms. Two important roles of the telomeres are to prevent chromosome end-to-end fusion and loss of genetic information. While the gradual …
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Engineering structural variants with prime editing
… across the genome, thereby identifying essential coding and non-coding sequences. First, I built a predictive model for prime editing insertion efficiencies using experimental data generated in our laboratory. This model, called MinsePIE, forecasts insertion rates of sequences across various …
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Understanding the functional consequences of genetic variation on gene regulation
Understanding the relationship between the non-coding sequence of the genome and the gene expression is one of fundamental goals of regulatory genomics. Perturbing certain locations in the non-coding DNA causes a disturbance to the precise spatial and temporal expression of the genes. Gene …
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Understanding the role of CD4+ T cells in common immune-mediated diseases
… throughout the genome, most of which lie within non-coding DNA. Moreover, these loci are enriched in CD4+ T cell regulatory elements, which suggests they might disrupt the expression of nearby genes in T cells. Nonetheless, the target genes of most immune disease loci have yet to be discovered. …
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Investigating the role of rare genetic variants in the aetiology of haemostasis disorders
… each person. Furthermore, historically only the coding portion of the genome, or so-called exome, has been explored to identify causal variants. It is reasonable to assume that, for a fraction of patients with unexplained inherited diseases, the answer lies in the non-coding portion of the …
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Mining the Genetic Diversity of the Pathogenic Protozoan, Neospora caninum
… workflows, to identify sequence variants in coding and non-coding DNA. Annotation of variable regions would reveal potential virulence markers distinguishing isolates of this species. Challenges accompanying such research include the lack of optimisation and standardisation of NGS analysis …
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miR-137, schizophrenia and sleep regulation in Drosophila melanogaster.
Introduction – microRNAs are non-coding DNA sequences, which regulate gene expression by promoting the destruction of target mRNAs. The evolutionarily conserved miR-137 is expressed in invertebrate and mammalian neuronal tissue and there is experimental evidence for mir-137 regulating neurological …
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A Semi-Supervised Predictive Model to Link Regulatory Regions to Their Target Genes
… enhancers are specific DNA sequences that act as ``information integration hubs" to confer regulatory requirements on a given cell. These non-coding DNA sequences can regulate genes from long distances, or across chromosomes, and their relationships with their target …
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Genomic Characterization of Sickle Cell Mouse Models for Therapeutic Genome Editing Applications
… motif within this region by Cas9-mediated non-homologous end joining in human erythroid cells or their progenitors caused induction of HbF by interfering with recruitment of the transcriptional repressor, BCL11A. This strategy results in potent HbF induction in human cells and is a …
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Computational Prediction of Functional Elements through Comparative Genomics
… and the variabilities of the manners of encoding the information make it a very challenging task. Nucleotides mutations and genome-wide re-arrangements bring additional great challenges in identification and understanding of the functional elements in the genome. On the other hand, due to …
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Diagnostics and epidemiology of <i>Phytophthora infestans</i> : the cause of late blight of potato
… and INF REV, a 613 bp product was generated from DNA of an isolate of <i>P. infestans</i>. No product was amplified when DNA from isolates of various other <i>Phytophthora </i>species and potato blemish pathogens was tested, showing that the primers were species-specific. These primers can detect …
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Evolution in Neotropical Herpetofauna: Species Boundaries in High Andean Frogs and Evolutionary Genetics in the Lava Lizard Genus Microlophus (Squamata: tropiduridae): A History of Colonization and Dispersal
… with population level molecular (mitochondrial DNA) markers (Chapter 2). Our results indicate that the striking differences in size, coloration and general appearance in the various Lake Titicaca morphotypes are not genetically based. Further, there is evidence that these morphotypes have …
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Evolution of the Neckeraceae (Bryopsida)
… short. Therefore we chose to use mainly non-coding DNA sequences from rapidly evolving DNA regions. The phylogenetic reconstructions are based on extensive sequence data from all genomes: plastid trnS-trnF and rpl16, nuclear ITS1 & 2 and mitochondrial nad5. Both parsimony (PAUP and …
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Elucidation of the Molecular Mechanisms Underlying Estrogen-Mediated Estrogen Receptor Activation
… material is interpreted. Hidden in the 98% of non-coding DNA sequences, once referred to as “junk DNA,” are the instructions for how to turn genes on and off (i.e., the operating system). As in any other language, decoding the instructions between DNA and gene expression is the key for …