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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"nitrite reductase"”.
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Evolution of copper-containing nitrite reductase
Copper-containing nitrite reductase (NiR) is a homotrimer of two cupredoxin domains and catalyzes the single electron reduction of NO2- to NO during dissimilatory denitrification. To investigate the evolution of NiR, methods of mutagenic library generation and high-throughput variant screening from …
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Nitrate- and Nitrite-Reductase Activities in Mycobacterium Avium A5
… medium (Nitrogen Test Medium) with glutamine, nitrite, nitrate, or ammonia as sole nitrogen source at a concentration of 2 mM at 370C aerobically. The strain grew well on all the nitrogen sources except nitrite. It grew slowly on nitrite with a generation time of 6 days and cultures were not …
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Structure/function relationships of the copper proteins nitrite reductase and rusticyanin
… relationships of two copper proteins, nitrite reductase and rusticyanin using a combined approach of site-directed mutagenesis and X-ray crystallography. Dissimilatory nitrite reductase (NiR) catalyses the reduction of nitrite to nitric oxide (NO) as part of the key biological process …
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Elucidating the Roles of Conserved Active Site Amino Acids in the Escherichia coli Cytochrome c Nitrite Reductase
The periplasmic cytochrome c nitrite reductase NrfA is a homodimeric protein containing ten c-type cytochromes. NrfA catalyses the six electron reduction of nitrite to ammonia which in turn facilitates anaerobic respiration. NrfA also reduces nitric oxide and hydroxylamine to ammonium. The …
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Novel Electron Transfer Systems in Hyperthermophilic Methanogenic and Anaerobic Methanotrophic Archaea: F420-dependent Nitrite Reductase and [Fe-S] Cluster Assembling Thioredoxin
… survival strategies, an F420-dependent sulfite reductase (Fsr), deazaflavin-dependent flavin-containing thioredoxin reductase (DFTR), and a thioredoxin (Trx) homolog with a new function. These proteins are involved in redox reactions, and the research presented here deals with the biochemistry …
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Studies of the effect of cysteine and heme pocket mutations of the nitric oxide dioxygenase and nitrite reductase activities of human Cytoglobin and Androglobin heme domain
… tissues have been an area of interest, the nitrite reductase activity (NiR) and the nitric oxide dioxygenase activity (NOD) working in tandem is a proposed regulatory response which enhances oxygen levels in hypoxic tissues, serving to protect tissues against hypoxia. In hypoxic conditions, …
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The electron transport chains of Neisseria meningitidis
… conditions, whereas oxidation of cytochromes by nitrite was only seen in cells grown under denitrifying conditions, and the predominant oxidizable cytochromes were b-type. These are likely to be associated with the oxidation of a b-haem containing nitric oxide reductase. Nitrite inhibits the …
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Perennial energy crops on riparian buffers affect nitrogen cycling microbial communities
… groups involved in ammonia oxidation (amoA), nitrite ammonification (nrfA), denitrification (nirK, nirS, norB, and nosZ), and N fixation (nifH). The results indicated that N application significantly affected the abundance of four different functional enzyme groups. The abundance of bacterial …
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The role of copper proteins in the ammonia oxidation pathway of Nitrosopumilus maritimus
… cupredoxin domain, and Nmar1667 an NO-forming nitrite reductase. Purification of Nmar1131 was performed. Originally over-expression was attempted in a cytoplasmic vector, which proved unsuccessful. To echo the predicted native periplasmic nature of this protein, the nmar1131 gene was cloned …
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Magneto-optical studies of RirA and cytochrome cd1
… per monomer. Cytochrome cd1 is a homodimeric nitrite reductase found in denitrifying bacteria. Each monomer binds a heme c that transfers electrons to the structurally unique heme d1 at the active site. It is shown that the inactive as-prepared form of cd1 from Paracoccus pantotrophus is …
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Geomicrobiology of nitrogen in a coastal aquifer : isotopic and molecular methods to examine nitrification and denitrification in groundwater
… including many potential denitrifiers. However, nitrite reductase, nirS and nirK, genes were also recovered from the sediments with nirK dominating in the surface sediments. This contrasts with the deep salt wedge, where the microbial community 16S rRNA genes appear more closely related to marine …
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Oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production
… ABC transporter ATP-binding protein, nitrate reductase, and nitrite reductase. The delignification ability was tested on its chemical composition. Methods used followed technical association pulp and paper industry TAPPI T 222 om-02 (lignin content), Kurscher-Hoffner (holocellulose and …
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Trimethylamine-N-oxide reduction by alteromonas spp.
… In Alteromonas sp., NGMB 400, the enzyme TMAO reductase was induced by TMAO, and repressed by oxygen and fumarate but not nitrate. Membrane-bound cytochrome C552 and TMAO reductase were simultaneously induced. TMAO reductase was located predominantly in the periplasm, but difficulties were …
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Exploring the Molecular Mechanisms of Human Cytoglobin and its Role in Cancer
… reaction to environments including NiR (nitrite reductase), lipid and liposome interactions and NOD (nitric oxide dioxygenase). The WT protein was compared to results gathered from two mutants: H81A and L46W. H81 mutation was selected as it affects NiR activity in neuroglobin and L46 …
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Geomicrobiology of nitrogen in a coastal aquifer : isotopic and molecular methods to examine nitrification and denitrification in groundwater
… including many potential denitrifiers. However, nitrite reductase, nirS and nirK, genes were also recovered from the sediments with nirK dominating in the surface sediments. This contrasts with the deep salt wedge, where the microbial community 16S rRNA genes appear more closely related to marine …
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EFFECTS OF RED BLOOD CELLS ON NITRIC OXIDE BIOACTIVITY
… to 70 folds. Under hypoxic and acidic condition, nitrite is reduced to NO by deoxygenated hemoglobin. Since two states of Hb (R-state and T-state) are reversible under proper condition, Hb acts as an allosterically controlled nitrite reductase. Using electron paramagnetic resonance spectroscopy, …
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Optimizing electrogenic activity from photosynthetic bacteria in bioelectrochemical systems
… bi-directional hydrogenase – HoxH, nitric oxide reductase – NorB, cytochrome-c oxidase – COX, bd-quinol oxidase – cyd, and the respiratory terminal oxidase – ARTO, roughly doubled light driven electron flux to EET. Deletion of nitrate reductase – NarB, and nitrite reductase – NirA, increased EET …
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The evolution and diversity of noncanonical microbial nitrogen metabolisms
… I report a novel cytochrome-type nitrite reductase (nirS) structure within members of Phylum Chloroflexi, which includes a cytochrome superfamily domain in addition to functional domains conserved in Proteobacterial nirS. Phylogenetic domain mapping reveals that this gene resulted …
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Characteristics of Nitrogen- and Iron-Limited Growth in the Diatom Phaeodactylum tricornutum, and in Natural Phytoplankton Populations.
… due to the extra costs associated with nitrate reductase (NR) and nitrite reductase (NiR) activity. The metabolic costs of growth on nitrate may be significantly increased under iron (Fe)-limitation, as both NR and NiR require Fe. Fe-limited chemostat cultures excreted nitrite and ammonium when …
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Effects of Nitrate and Cytokinin on Nitrogen Metabolism and Heat Stress Tolerance of Creeping Bentgrass
… of HSPs. The transcript abundance of nitrate reductase (NR), nitrite reductase (NIR), plastidic glutamine synthetase (GS2), ferredoxin-dependent glutamate synthase (Fd-GOGAT), and glutamate dehydrogenase (GDH) and N metabolites in shoots were monitored during the day/night cycle (14/8 h). All …
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