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Showing 1 to 11 of 11 for “"nitrile hydratases"”.
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Mechanistic studies of nitrile hydratases and their activator proteins
Nitrile hydratases (NHases) are iron- or cobalt-containing metalloenzymes with a hexadentate active site characterized by a highly conserved CXXCSC motif on the α subunit. Two of the active site cysteine residues undergo post-translational oxidation to sulfenic and sulfinic acid moieties. Both the …
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Investigating the Biochemical and Catalytic Properties of Nitrile Hydratases
… is typically achieved by the hydration of nitrile compounds under acidic or basic conditions, high temperatures, and copper catalysts. However, the use of such chemical methods leads to the generation of unwanted by-products and toxic wastes, in addition to low product yields and high …
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Mechanistic studies of nitrile hydratases and their activator proteins
Nitrile hydratases (NHases) are iron- or cobalt-containing metalloenzymes with a hexadentate active site characterized by a highly conserved CXXCSC motif on the α subunit. Two of the active site cysteine residues undergo post-translational oxidation to sulfenic and sulfinic acid moieties. Both the …
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Screening for novel cold-adapted nitrile hydratases in Antarctic metagenornic libraries
Nitrile hydratases (NHase) catalyse the hydrolysis of nitriles to their corresponding amides, the first step in the nitrile degradation pathway. The second step is the conversion of the amide to a carboxylic acid, catalysed by an amidase. The NHases published to date have been isolated from …
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The crystal structure of a mutant nitrile hydratase
Nitrile hydratases can be used as industrial biocatalysts. They catalyze the conversion of nitriles to their corresponding amides. These industrial biocatalysts have recently gained attention due to the economic production of industrial commodities such as acrylamide and nicotinamide (Thomas et …
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Investigation of the determinants of thermal stability of the nitrile hydratase from Geobacillus pallidus RAPc8
… to enhance their application in industry. The nitrile hydratases group of enzymes catalyse the hydrolysis of nitriles to amides using an exothermic catalytic mechanism. Understanding and applying specific amino acid residue mutations at specific regions in protein structures has been important …
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An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8
Nitrile hydratases (NHases) are bacterial metalloenzymes that catalyze the hydration of nitriles to their corresponding amides. The enzymes have been found in several microorganisms and participate in the metabolism of nitrile compounds as source of carbon and nitrogen. The commercial use of NHases …
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Catalytic Mechanism and Maturation of the Metalloenzyme Nitrile Hydratase
<p>Nitrile hydratases are metalloenzymes that catalyze the hydration of nitriles to their corresponding amides in a specific manner at ambient pressures and temperatures at neutral pH. Traditional industrial methods require high temperature and pressure, extreme pH, and heavy metals. NHases are …
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The structure, function and engineering of a thermostable nitrile hydratase
Nitrile hydratases (NHases) are enzymes that catalyse the conversion of organocyanides to amides via a non-hydrolytic hydration reaction. They are industrially relevant enzymes, currently used in the manufacture of nicotinamide and acrylamide. The target of this study belongs to the thermophilic …
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The evolution and diversity of noncanonical microbial nitrogen metabolisms
… prokaryotic distributions of other nonhomologous nitrile-reducing enzymes, thiocyanate hydrolases and nitrile hydratases. Finally, I detail the evolution of promiscuous nitrogen metabolism in nitrogenases. Though highly specialized for dinitrogen reduction, molybdenum and vanadium nitrogenases …
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The relationship between structure and thermostability of a nitrile hydratase from Goebacillus pallidus RAPc8
Nitrile hydratases (NHases) are very important biocatalysts for the enzymatic conversion of nitriles to industrially important amides such as acrylamide and nicotinamide. An “ideal” NHase should fulfil several essential criteria including, high substrate conversion rates, being able to tolerate …