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Showing 1 to 20 of 50 for “"nisin"”.
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Bioengineering strategies to improve functional qualities of nisin
… as one such alternative to classic antibiotics. Nisin A belongs to the subgroup of bacteriocins called the lantibiotics, which contain several unusual amino acids as a consequence of enzyme-mediated post-translational modifications. As nisin is produced by generally regarded as safe (GRAS) …
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The cellular and mechanistic study of nisin inhibition of bacillus anthracis spore outgrowth, nisin alteration of infection, and native producer immunity
Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2011-05-25T14:30:06Z Item is restricted until 2013-05-25T14:29:35Z
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Bioengineering of nisin to enhance functionality against dairy pathogens
… were generated from the ‘hinge’ region of the nisin peptide. Furthermore, a derivative in which the three hinge amino acids were replaced with three alanines represents the first enhanced derivative of nisin to have been designed through a rational process. This derivative also formed the …
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Structural and Biochemical Studies of Nisin *Biosynthesis and Autoimmunity
The lantibiotic nisin is a 34-residue bacteriocin produced by the Gram-positive bacteria Lactococcus lactis spp. It is first ribosomally synthesized as an inactive precursor which is then post-translationally modified into its mature biologically active form. Described herein is a two-part study …
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Improving the efficacy of nisin to control Listeria monocytogenes in queso fresco
… antimicrobial treatments that can work in QF. Nisin is an antimicrobial peptide produced by certain strains of Lactococcus lactis, it is widely used in the food industry and it is able to inhibit L. monocytogenes, however it has limited activity in QF. Therefore, the overall goal of this study …
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Antimicrobial efficacy of nisin, oregano and ultrasound treatments against foodborne pathogens in ready-to-eat vegetables
Submitted in fulfillment of the academic requirements for the Master of Applied Sciences in Food Science and Technology degree at the Department of Biotechnology and Food Science, Faculty of Applied Sciences Durban University of Technology, Durban, 2020.
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Starch based systems for the colonic delivery of bioactive peptides
… the well characterised antimicrobial peptide nisin was used; this peptide is normally digested during GI transit. To prepare the nisin a simple purification process was developed, which produced a powder containing ∼33% nisin from a nisin producing culture and also enriched a commercial nisin …
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Technological features and ability to produce nisin by Lactococcus lactis subsp. lactis bv. diacetylactis obtained from dairy environment
… to PCR to detect bacteriocin-related genes (nisin, lacticins 481 and 3147, lactococcins A and 972) and further investigated by PCR and sequencing for the nisin operon. Cell free supernatants (CFS) of nisin-positive strains were tested by the spot-on-the lawn assay against a panel of 16 …
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Elimination of Listeria monocytogenes in a Soft Cheese, Fromage Blanc, Using Processing Methods, Formulation Changes, and Additive Bacteriocin Nisin
… addition of the anti-listerial bacteriocin nisin (Danisco Nisaplin) in formulation at different levels (125 ppm, 250 ppm, 400 ppm), and combinations of these treatments. Characteristics including pH, fat content, protein content, and color were evaluated for each treatment cheese. …
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Characterisation of novel bacteriocins from canine sources
… investigates the heterologous expression of the nisin immunity proteins NisI and NisFEG, and the nisin resistance proteins Nsr and NsrFP, in three Lactococcus lactis strains. One strain is nisin sensitive (MG1614), one is immune (NZ9800), and the third is both nisin producing and immune (NZ9700). …
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The Efficacy of Antimicrobials for the Control of Alicyclobacillus acidoterrestris in Fruit and Vegetable Juices
… 75 and 25 ppm (lysozyme); and 5, 3, and 1 IU/ml (nisin). In apple juice, A. acidoterrestris population reductions were caused by the following antimicrobials (reduction in log CFU/ml): lysozyme - all levels and nisin - 5 IU/ml (5.1), nisin - 3 IU/ml (4.2), cinnamic acid - 125 ppm (3.1), cinnamic …
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INTERACTION AND PROTECTION OF ANTIMICROBIAL COMPOUNDS WITH CARBOHYDRATE-BASED COLLOIDAL SYSTEMS FOR IMPROVED FOOD SAFETY
… first part, we examine the interactions between nisin and carbohydrate based materials in non-emulsion (aqueous) and emulsion systems. Phytoglycogen octenyl succinate (PG-OS), a dendrimer-like amphiphilic material was used as the model carbohydrate nanoparticle and nisin was used as the model …
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INTERACTION AND PROTECTION OF ANTIMICROBIAL COMPOUNDS WITH CARBOHYDRATE-BASED COLLOIDAL SYSTEMS FOR IMPROVED FOOD SAFETY
… first part, we examine the interactions between nisin and carbohydrate based materials in non-emulsion (aqueous) and emulsion systems. Phytoglycogen octenyl succinate (PG-OS), a dendrimer-like amphiphilic material was used as the model carbohydrate nanoparticle and nisin was used as the model …
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Biomedical applications for bacteriocins in infection and oncology
… in multiple settings has grown in recent years. Nisin in particular has been the subject of many studies due to it being well characterised, approved by the FDA, widely used as a food preservative (EU number E234) and having GRAS (generally regarded as safe) status. Nisin has bioengineered to …
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Exploring and understanding lantibiotic biosynthesis
… of cysteines to the dehydrated residues. Nisin, a class I lantibiotic produced by Lactococcus lactis, is one of the oldest known antibiotics. In this study, we have developed a production strategy for class I lantibiotics in Escherichia coli and show successful production of nisin as a …
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Controlling bacterial contaminants in sugarcane ethanol fermentations
… of the antimicrobials hen egg white lysozyme, nisin and the combination of nisin and penicillin against representative contaminants a model system was developed. To mimic the typical Brazilian conditions, the model incorporates high initial yeast inoculum, fermentation times of twelve hours or …
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Control of pathogenic bacteria in Queso Fresco by using generally recognized as safe ingredients
… ingredients. These antimicrobial agents included nisin (Nisaplin®), caprylic acid, trans-cinnamaldehyde, monolaurin, carvacrol, eugenol, levulinic acid, orange-terpenes, d-limonene, eucalyptol, thymol, and clove essential oils. Batches of queso fresco were inoculated with mixtures of approximately …
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Microfluidic assembly of zein microcapsules
… analysis. Finally, an antimicrobial peptide nisin was encapsulated as an example to demonstrate an application of this technology. Nisin is a natural antimicrobial agent that can inhibit the growth of Listeria monocytogenes. However, the application of nisin has the limitation due to its …
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