Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"nile red"”.
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Investigating optical microplastic detection methods using fluorescent staining through Nile Red
… MPs. Fluorescent tagging of microplastics using Nile Red (NR) has recently emerged as an accessible and popular detection method. However, robust, standardized methods of using Nile Red to identify between plastic and organic materials or distinguish between polymers are still being developed. …
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Part I: In-situ fluorometric quantification of microalgal neutral lipids Part II: Thermal degradation behavior of investment casting polymer patterns
… a lipophilic fluorescent dye. The traditional Nile red stain-based method for detecting microalgal intracellular lipids is limited due to varying composition and thickness of rigid cell walls. In this study, the addition of dilute acid and heating of solution, were found to greatly enhance …
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Investigating the regulation of tumour associated lipogenesis using the model organism, Saccharomyces cerevisiae
… HOG1 pathway in neutral lipid accumulation, a Nile red assay was undertaken to measure lipid accumulation in stationary phase of genomic deletion strains of key HOG pathway proteins. Deletion of the majority of HOG components had no effect on lipid accumulation. However, the hog1Δ mutant showed …
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Root adaptive responses of tall fescue (Festuca arundinacea) growing in sand treated with petroleum hydrocarbon contamination
… persisted. The fluorescent hydrophobic probe „Nile red‟ was applied to the epidermis of a living root to mimic and visualise the uptake of naphthalene into the root through the transpiration stream. The root sections were also stained with 0.1% (w/v) berberine hemisulphate in order to stain …
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Interactions of ferriprotoporphyrin IX with neutral lipids and detergents: insights into their role in β-haematin formation
… parasite ingests between 80-100% of the host red blood cell's iron in the form of haemoglobin, which is catabolised to amino acids and ferriprotoporphyrin (Fe(III)PPIX), that it subsequently detoxifies to form the bio crystal, haemozoin. Neutral lipids have been implicated as the biological …
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Synthesis and characterization of pH/temperature dual sensitive multiblock copolymer for drug delivery
… and falling ball method. The polymer shopH-triggered degradation and drug release with nile red and irinotecan. The ketal linker connecting Pluronic® was cleaved and resulted in smaller molecular weight strain causing change in sol-gel transition temperature and critical micelle concentration. …
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Poly(ethyl glyoxylate) Solid-Core Particles for Drug Delivery
… cargo to be released upon stimulus-triggered cleavage of the PEtG end-cap. The PEtG-PLA NPs were formed using an oil-in-water emulsion-evaporation technique. Particles responsive to stimuli including UV light and reducing conditions were prepared and studied. Cleavage of the end-caps of …
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Effects of Polydatin on In Vitro Bovine Embryo Developmental Competence, Metabolism, and Cryopreservation
… from poor developmental competence and altered metabolism which hinders their cryotolerance. Overall, the goals of this thesis were to improve oocyte and embryo culture with the antioxidant polydatin and to optimize slow freezing procedures. This thesis was designed as three experiments, and …
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USE OF MICROALGAE FOR WASTEWATER TREATMENT AND BIOFUEL PRODUCTION: EFFECTS OF ENVIRONMENTAL CONTAMINANTS ON THE CELLULAR RESPONSE
… enhances the biofuel yield. This research explored the potential of microalgae to be used for the removal of wastewater pollutants while accumulating high concentrations of neutral lipid that can be converted into biodiesel. Physiological and transcriptional responses of two genera of green …
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The Effect of Constant Relative Growth Rate Versus Constant Specific Growth Rate on Oocystis SP
… concentration, lipid concentration using Nile Red stain, and the ash free dry weight. The results of this study indicated that the cultures grown most likely did not undergo nutrient stress except during GS1 at 20°C. Given the results under these conditions, neither GS1 nor GS2 is better …
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Forward and inverse metabolic engineering strategies for improving polyhydroxybyrate production
… a staining protocol that quantitatively related nile red fluorescence to PHB content, while maintaining cell viability for both organisms. This was combined with fluorescence activated cell sorting (FACS) to screen for high PHB mutants. A Synechocystis insertion mutagenesis library was screened …
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Toxicity and drug delivery ability of bio-inspired silica nanoparticles
… This work focused on the use of bio-inspired silica as a potential drug delivery agent and the toxic effect produced due to their small size. Using two bio-inspired drug encapsulation methods and a range of amines the encapsulation and release profiles of hydrophilic Calcein and …
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Zein-based nanoparticles for drug delivery applications
… could deliver a model hydrophobic substance, Nile red, into B16-F10-luc-G5 melanoma cells in a time-dependent manner, with higher cellular uptake observed when using smaller chain length PEG5K and lower PEG density. The impact of the protein corona on the uptake of PEGylated zein micelles by …
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Fluorescence photophysics, characterisation and application of labelled glucose/galactose binding protein
… effects on the sensor function. The dyes Texas Red and iodoacetamide nile red (IANR) were attached to the GBP binding site to investigate the possibility of producing an alternative, longer-wavelength sensor system than when using BADAN. The ostensibly environment-insensitive Texas Red dye …
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Effect of UV-A irradiance on lipid accumulation in Nannochloropsis oculata
… was done using the lipid soluble fluorescent dye Nile Red. UV-A treatments significantly increased the chlorophyll-specific lipid concentration of <italic>N.oculata</italic> cells, and I was unable to reject that reciprocity holds. The third objective was addressed by a pair of factorial bioassay …
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Advanced Fluorescence Lifetime Imaging and Spectroscopy Techniques for Biological Samples
… equation. The intracellular viscosity is measured via FLIM, time-resolved and steady-state fluorescence anisotropy. The relationship between the fluorescence lifetime and rotational correlation time of BODIPY-Ci2 in methanol/glycerol mixtures agrees with the combination of the Forster-Hoffmann …
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Development of mechano-active organic materials for shock wave energy dissipation
… classes of organic materials capable of volume reductions upon exposure to external stimuli, such as mechanical force, temperature, and light, have been developed as potential candidates for SWED. Chapter 2 explores the feasibility of shock-induced intramolecular volume collapse via the …
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Synthesis and self-assembly of multiple thermoresponsive amphiphilic block copolymers
… was synthesized which differed only in the relative size of the two blocks. Depending on the relative block lengths, different aggregation pathways were found. Furthermore, the complementary TMS-labeled end groups served as NMR-probes for the self-assembly of these diblock …
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Algae and cyanobacteria platforms for engineering biofuel production
… with nutritional triggers were used to redirect carbon into lipid biosynthesis. Wild-type and starch mutants of Chlamydomonas reinhardtii, a well-established model green alga, were subjected to nitrogen starvation to examine carbon storage and partitioning between starch and lipid …
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Algae and cyanobacteria platforms for engineering biofuel production
… with nutritional triggers were used to redirect carbon into lipid biosynthesis. Wild-type and starch mutants of Chlamydomonas reinhardtii, a well-established model green alga, were subjected to nitrogen starvation to examine carbon storage and partitioning between starch and lipid …
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