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Showing 1 to 8 of 8 for “"nif genes"”.
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Mapping nif genes of Rhodobacter capsulatus, and characterization of a mutant with a pleiotropic defect in nitrogen metabolism
… Rhodobacter capsulatus to fix nitrogen (Nif- ) have previously been mapped into six linkage groups (I-VI) by transduction with gene transfer agent (GTA). Because this vector carries only 4600 bp of DNA, linkage can only be demonstrated for short chromosomal distances. In order to …
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Characterization and site-directed mutagenesis of NifU from Azotobacter vinelandii
In order to elucidate the function of the nifU gene product in nitrogenase maturation in Azotobacter vinelandii. the gene product has been hyperexpressed in Escherichia coli and characterized by various biophysical techniques. Following the initial characterization, site-directed mutagenesis of …
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Nitrogen Fixation By Azotobacter Species In Mixed Culture
… mixed cultures of A. chroococcum did fix a significantly greater amount of nitrogen (164 ug/ml) than the pure culture of A. chroococcum (134 ug/ml). The specific increase in fixed nitrogen appears to be a function of the ability of the added "contaminant" both to utilize ammonia at low …
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Nitrogen Metabolism and Solvent Production in Clostridium Beijerinckii Nrrl B593
… analysis of the mRNA of the solvent-producing genes in samples harvested periodically from a nitrogen-fixing culture of C. beijerinckii showed the presence of both enzyme activities and the mRNA carrying the solvent-production genes throughout incubation. A 2.5-fold increase in the specific …
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Studies on the structure and function of various nif and nif- associated gene products encoded within the Azotobacter vinelandii nif gene cluster
… was developed for oligonucleotide-directed mutagenesis of these proteins and the resulting biological and biochemical effects of these changes were examined. Identification of structurally important regions in the MoFe protein subunits and assignment of specific amino acid residues as potential …
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Structural and Functional Characterization of Cyanoglobin: A Peripheral Membrane Hemoglobin in Nostoc commune UTEX 584 (Cyanobacteria)
Investigations of the nitrogen fixing (nif) genes in the cyanobacterium Nostoc commune UTEX 584 revealed a gene encoding a hemoprotein, named cyanoglobin. The cyanoglobin gene was isolated and subcloned into Escherichia coli previously. Cyanoglobin possesses a high oxygen affinity. The study …
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Diversity of Free-living Nitrogen Fixing Bacteria in the Badlands of South Dakota
… (NMF) under hypoxic conditions. The 16srRNA and nifH genes were amplified and sequenced. This was complemented using a culture independent approach, amplifying nifH from soil DNA extracts. The 16S rRNA gene data indicated a diversity of putative free-living diazotrophs across 4 phyla …
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A Study of the Genetics of Root Nodulation in Pigeonpea (Cajanus cajan) Using Indigenous Rhizobia
… distinct morphological characteristics and significant differential N fixation ability of the pigeonpea genotypes suggesting that there was a potential to select for optimum host genotype x rhizobial strain combinations for N fixation in this legume species. In the second objective of the study …