Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"nicastrin"”.
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Intramembrane Proteolysis Mediated by the gamma-Secretase Complex : Nicastrin Functions as a Substrate Receptor
… a complex of proteins that include presenilin, nicastrin, Aph1, and Pen-2; (2) catalyzes the intramembrane cleavage of a broad range of substrates, regulating physiology from neurodevelopment to neurodegeneration. The aim of this thesis is to elucidate the mechanism by which the gamma -secretase …
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Neurogenesis and Gliogenesis from Ascl1 (Mash1) Expressing Progenitors in the Central Nervous System
… In chapter four, the physiological functions of Nicastrin in gliogenesis were investigated. Nicastrin is a requisite subunit of the !-secretase complex essential for activating Notch signaling pathway. Conditional mutant of Nicastrin leads to the increased level of oligodendrocytes lineage …
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Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease
… However, the zebrafish orthologue of the NICASTRIN gene has not been studied in detail. In Chapter II, we characterize in zebrafish the orthologue of the human NICASTRIN (NCSTN) gene that encodes a protein component of the γ-secretase. We demonstrate the spatial and temporal expression …
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Novel Mechanisms of Antigen Processing That Enhance Bcg Vaccine Efficacy
… of the components of the protease namely, nicastrin, presenilin and APH, which led to a decrease in antigen presentation. We therefore conclude that, even though BCG phagosomes are sequestered and do not fuse with lysosomes to generate peptide antigens, there are complex and novel <em>in …
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Investigating β- and γ-secretase function and processing of APP with genetic and pharmacological tools
… Here, I demonstrate the endogenous tagging of Nicastrin, an integral subunit of γS, with the fluorescent protein mEmerald in human cells using CRISPR-Cas9, allowing for subcellular visualization of γS distribution in real time. I also test the ability of an optogenetic nanobody to perturb …
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Identification of the cellular mechanisms undelying the contribution of stress and glucocorticoids to Alzheimer's disease pathology
… the expression of APP-cleaving enzymes (BACE and nicastrin), giving rise to the APP C-terminal fragment 99 (C99) which, after further processing, yields amyloid beta (A). While A is the APP product to which AD pathology is usually ascribed, C99 also has deleterious effects on neuronal survival …
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Reconstructing the gamma-secretase complex using the MultiBac baculovirus system for expression of heterologous multi-protein complexes
… Four core proteins, namely Presenilin (PS), Nicastrin (NCT), Anterior-pharynx defective homolog-1 (aph1aL/ aph1aS/ aph1b) and Presenilin enhancer-2 (pen2), interact to form the active γ-secretase complex. The assembly and function of the enzyme components are slowly becoming apparent. …