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Showing 1 to 4 of 4 for “"nfa1 gene"”.
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Knockdown of nfa1 Gene Cloned from Naegleria fowleri by Antisense RNA
… in vitro cytotoxicity of N. fowleri. The nfa1 gene, cloned from a cDNA library of N. fowleri by immunoscreening, should be concerned with the formation of food-cups that is a phagocytic structure. In addition, an anti-Nfa1 antobody reduced the in vitro cytotoxicity of N. fowleri against …
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The effect of nfa1 accination against Naeglearia fowleri infection
… in experimental animals and in humans. The nfa1 gene that is cloned from N. fowleri is located on pseudopodia, especially amoebic food-cups and plays an important role in the pathogenesis of N. fowleri. In this study, the retroviral vector (pQCXIN) and the lentiviral vector (pCDH) cloned …
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Cytopathic Effects of a Pathogenic Naegleria fowleri and an Anti-Nfa1 Antibody on Rat Microglial Cells
… of amoeba to host cells is most important. The nfa1 gene (360 bp) was previously cloned from a cDNA library of pathogenic N. fowleri by immunoscreening, and produced a 13.1 kDa recombinant protein (rNfa1) that showed the pseudopodia specific localization by immunocytochemistry. On the basis of …
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Role of the Nfa1 Protein in Pathogenic Naegleria fowleri Co-cultured with Target Cells
… mouse sera, we previously cloned an antigenic gene (called nfa1) from a cDNA library of N. fowleri by immunoscreening. The nfa1 gene had the coding sequence of 360 bp, producing a 13.1kDa recombinant protein (rNfa1). Anti-Nfa1 polyclonal antibody, revealed pseudopodia-specific …