Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"neutrophil elastase"”.
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Imbalance Between Neutrophil Elastase and Elafin Promotes Breast Cancer Growth and Progression
… component of the epithelial barrier against neutrophil elastase (NE) activity. The central hypothesis examined in this dissertation was that elafin has tumor suppressive properties in breast cancer. In support of this hypothesis, immunohistochemical (IHC) analysis revealed that elafin was …
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Doxorubicin-Induced Cardiotoxicity Is Mediated By Neutrophils Through Release of Neutrophil Elastase
… and innate immune cells, in particular neutrophils. Recognizing that neutrophils have been implicated in a number of heart diseases, we evaluated the role of neutrophils in Dox-induced cardiotoxicity. Here, using echocardiography, flow cytometry and immunofluorescence staining, we …
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Modulation of dendritic cells by human neutrophil elastase and its inhibitors in pulmonary inflammation
… to microbial infections and increased neutrophil elastase (NE) in lung secretions. This thesis was designed to test the hypotheses that; (i) excess NE may result in a dysregulation of lung DCs function in pulmonary chronic diseases, and (ii) the natural NE inhibitors in the respiratory …
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Characterizing The Source of Neutrophil Elastase and Proteinase 3 Cross-Presentation In B-Cell Acute Lymphoblastic Leukemia
… antigen derived from serine proteases neutrophil elastase (NE) and proteinase 3 (P3). NE and P3 are primarily expressed in cells of myeloid lineage including granulocytes, bone marrow progenitors and myeloid leukemia. Our lab reported that NE and P3 are cross-presented by antigen …
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The synthesis and evaluation of valine mimic protio and halo enol lactones as human neutrophil elastase inhibitors
Human neutrophil elastase (HNE) has been found to be responsible for the destruction of lung tissue in emphysema. Based on the primary substrate selectivity of HNE, a series of valine mimic protio and bromo enol lactones were designed as HNE inhibitors. General methods were developed for the …
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Bioengineering the Expression of Active Recombinant Human Cathepsin G, Enteropeptidase, Neutrophil Elastase, and C-Reactive Protein in Yeast
… all other reported variants of this enzyme. The neutrophil serine proteases human cathepsin G (hCatG) and human neutrophil elastase (HNE) were expressed in P. pastoris and HNE was also expressed in K. lactis. The recombinant variants rhCatG and rHNE, with intact C-terminal extensions, were …
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Novel findings on cellular trafficking and targeting for granule storage of neutrophil elastase, a multifunctional effector molecule of innate immunity
Neutrophil elastase (NE) has important roles in innate immunity, killing pathogens and controlling the immune response; but how NE is targeted to developing granules is not understood. Therefore, the aim of this thesis was to investigate the sorting of NE. Transfection experiments in a leukemic …
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Cellular Uptake of Neutrohpil Elastase Links Inflammation to Adaptive Immunity
… in the development and progression of cancer. Neutrophils are primary mediators of the innate immune response. Upon activation, an important function of neutrophils is release of an assortment of proteins from their granules including the serine protease neutrophil elastase (NE). The effect of …
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Role of Type III TGF-β Receptor Shedding in Regulating Tumorigenesis
… have also demonstrated that cathepsin G and neutrophil elastase, proteases stored in the azurophil granules of neutrophils, can induce cleavage of cell surface TβRIII though no functional sTβRIII was detected. Similarly, activated neutrophils could induce TβRIII shedding on cancer cells. …
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Serpin polymers enforce molecular filtration in the endoplasmic reticulum
… in lungs where its major function is to inhibit neutrophil elastase. Neuroserpin is a serine protease inhibitor expressed in the nervous system. Mutations in these two proteins can cause them to polymerise and accumulate within endoplasmic reticulum (ER) causing its fragmentation into ER …
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Cross-Presentation Is A Source of Tumor Antigens For Multiple Myeloma Immunotherapy
… (HLA)-A2 nonameric peptide that is derived from neutrophil elastase (NE) and proteinase 3 (P3), and the ability to treat multiple myeloma using PR1-targeting immunotherapies. Here we demonstrate that multiple myeloma cells lack endogenous NE and P3 expression, possess the ability to take up …
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PERINATAL CELLS AS A TREATMENT FOR INFLAMMATORY LUNG DISEASES
… immune cell counts in their lungs such as neutrophils, macrophages and T cells accompanied by elevated levels of pro-inflammatory cytokines such as IFN-γ, IL-6, IL-8, IL-1β and neutrophil elastase (NE). Increased immune cell numbers and increased levels of their associated cytokines in the …
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Antimicrobial and immuno-modulatory peptides as a treatment for lung disease and delivery by nebulisation
… cleavage. Cleavage predictions highlighted neutrophil elastase (NE) as most likely responsible. All peptides were susceptible to cleavage by NE, and several peptides retained function following NE-incubation. Mass spectrometric analysis and cleavage predictions informed truncated derivative …
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Identification and validation of Biomarkers for Breast Cancer from human White Blood Cells
… patients. The levels of SERPINB1 (Serpin B1, neutrophil elastase inhibitor) and CPNE3 (Copine 3, phospholipid binding protein) were assessed using Western blotting. These analyses demonstrated the association of SERPINB1 with breast cancer metastases, and suggested its potential utility as a …
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Protein FOG at the interface between G streptococci and human host defence lines
… Intact protein FOG mediated aggregation of neutrophils in the presence of fibrinogen which disabled these cells in excerting antibacterial activities. In vitro and in vivo, protein FOG interacts with collagen I, an abundant extracellular matrix protein of human skin. As streptococcal skin …
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Hypoxia induces selective neutrophil degranulation to promote endothelial damage in COPD
Neutrophils are the key effector cells of innate immunity, rapidly recruited to defend the host against invading pathogens. They deploy pre-formed proteases packaged within cytoplasmic granules, generate reactive oxygen species and release neutrophil extracellular traps (NETs). However, these …
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NOVEL CINNAMIC ACID-BASED DEHYDROPOLYMERS FOR EMPHYSEMA: IN VITRO AND IN VIVO ASSESSMENT OF THEIR ACTIVITIES
… against oxidative stress, inflammation and elastase, some of the pathogenic mechanisms associated with this disease. Three in vitro inhibitory activity assays for oxidative stress, lung inflammation and neutrophil elastase (NE) were developed and used to identify the most potent triple …
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