Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 113 for “"neutralizing antibodies"”.
-
CHARACTERIZATION OF DENGUE NEUTRALIZING ANTIBODIES USING RECOMBINANT ANTIBODIES TECHNOLOGY
… EXPRESSION, AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES OBTAINED FROM TWO NATURALLY INFECTED DENGUE PATIENTS. THIS LED TO THE SUBSEQUENT DISCOVERY AND FURTHER CHARACTERIZATION OF AN ANTIBODY THAT POSSESSES POTENT NEUTRALIZATION CAPACITY AGAINST ALL FOUR DENGUE VIRUS SEROTYPES. IN PARALLEL, AN …
-
Structure-function of Dengue 2 virus envelope protein domain III with neutralizing antibodies
… in the form of vaccines and antiviral drugs. Neutralizing antibodies are a critical component of immune-mediated protection from disease caused by the DENVs and the envelope (E) protein is the primary target. Potency of neutralization has been correlated with the particular domain on the E …
-
Evolution-dependent SARS-CoV-2 vaccination success determined by neutralizing antibodies and reliability of point-of-care testing
SARS-CoV-2 spread from Wuhan in China all over the world and was first detected in February 2020 in Germany. Under biosafety level 3 conditions, we established a Vero cell-based cultivation system and isolated an early Wuhan-like type (wt) of SARS-CoV-2 and the variants of concern Alpha, Delta, …
-
Studies on the antibody repertoire in a Dengue virus immune subject and isolation of neutralizing antibodies by phage display technology
… Enhancement (ADE) by cross-reactive, non-neutralizing antibodies. Previous studies using mouse mAbs demonstrated that the Ectodomain III (EDIII) in the E protein of DENV is the primary target of the most potent neutralizing antibodies against DENV. Interestingly, the EDIII specific …
-
HIV-1 Immune Escape and Neutralizing Antibodies : Solid Phase Proteoliposomes Containing HIV-1 Envelope Glycoproteins as Antigens and Immunogens and HIV-1 Core Envelope Glycoproteins Deficient in T-Helper Epitopes
… as trimeric spikes and are the major targets for neutralizing antibodies. The design of envelope glycoprotein-based subunit vaccines has been frustrated by many viral immune escape mechanisms. Trimeric envelope glycoprotein formulations hold promise to overcome limitations of monomeric envelope …
-
A Structural Perspective of Antibody Neutralization of Dengue Virus
… against DENV correlates with the production of neutralizing antibodies against the envelope: E) glycoprotein.</p><p>To understand the role of antibodies in DENV infection, we sought to dissect the relationship between epitope and function. Virologic studies had identified that the most potently …
-
The Role of Anti-Viral Immune Response on the Therapeutic Efficacy of Oncolytic Virotherapy for High-Grade Gliomas
… revealed that the development of high titers of neutralizing antibodies against adenovirus serotype 5 can correlate with reduced patient survival. Moreover, analysis of tumor-infiltrating lymphocytes from another clinical trial using Delta-24-RGD revealed that patients developed dominant T-cell …
-
Development and Evaluation of an Isospora Suis Sporozoite Neutralizing Antibody Assay in Swine Testicular Cells
An in vitro Isospora suis sporozoite neutralizing antibody assay has been developed in swine testicular (ST) cells. This assay measures the titer of neutralizing antibodies present in sera, milk and monoclonal antibody culture fluids by determining both the amount of intracellular sporozoites by …
-
A Recombinant Vesicular Stomatitis Virus Expressing the Junin Virus Glycoprotein for Arenavirus Countermeasure Development
… IgG antibody titers as well as detectable neutralizing antibodies. We next evaluated the use of rVSVΔG-JUNVGP as a tool for the detection of JUNV neutralizing antibodies. Conventional methodologies for the detection and quantification of JUNV neutralizing antibodies have several …
-
Neutralizing antibody responses in HIV dual infection: lessons for vaccine design
… have been developed that elicit strain-specific neutralizing HIV antibodies in animal models, and are currently being evaluated in several human clinical trials. Identifying whether multiple immunogens could be administered to facilitate the broadening of responses represents a pivotal challenge. …
-
Defining mRNA-generated T cell memory in respiratory viral infection
… with influenza virus in the absence of neutralizing antibodies. This intermediate state of protection was associated with a lack of resident memory cells following initial vaccination but expeditated T cell recruitment and adaptation to the lung when compared to unexposed mice. Together, …
-
Modulating the Antigenicity and Immunogenicity of HIV-1 Envelope by a Conserved N-Linked Glycan
… sites that are targets for some broadly neutralizing antibodies (bNAbs). Previous work has shown that removal of a highly conserved potential N-linked glycan (PNLG) site at amino acid residue 197 (N7) on the surface antigen gp120 of HIV-1 envelope glycoprotein (Env) increases …
-
Antigenic profiling of the porcine reproductive and respiratory syndrome virus
… PRRSV-specific immunity, namely the weak virus-neutralizing antibody response upon infection or vaccination in pigs, in sharp contrast with the robust non-neutralizing antibody response. Chapter 1 summarizes the current literature on general aspects of the virus and the disease, PRRSV-specific …
-
Design and characterization of novel immunogens for AIDS vaccine development and evaluation of a sample inference method for NGS Illumina amplicon data
… in 2015. A vaccine that could induce broadly neutralizing antibodies (bnAbs) is hypothesized to be the most efficient way to halt the AIDS pandemic. However, the majority of attempts to elicit bnAbs with HIV-1 vaccine candidates have failed due to the extensive variability and complex …
-
Understanding the interplay between HIV-1 diversity, humoral immune responses and viral fitness
… antibody dependent cell cytotoxicity (ADCC) and neutralizing antibody (nAb) responses are both thought to be important responses to elicit through vaccination. This thesis characterises neutralizing response in two cohorts in Africa, and in a detailed study of one individual, elucidates the …
-
Virus tropism and neutralization response in SIV infection
… both intracellularly and extracellularly. Neutralizing antibodies appeared earlier in IV-infected animals than in IR-infected animals. In progressors, this early humoral immune response was accompanied by early appearance of neutralization resistant variants. The majority of monkeys with …
-
The Roles of Activin A and B in Liver Inflammation and Fibrosis
… spleen. We then neutralized activin A and B via neutralizing antibodies in our concanavalin A-induced liver injury model to determine if inhibition of these ligands may confer protective effects during the acute inflammatory response in liver. Neutralization of either activin A or activin B …
-
Proteins Inside Cells and On the Surface of Membranes: Developing Three Dimensional In-Cell NMR and Structural Characterization of a Trimeric Membrane Proximal External Region Construct from HIV-1 gp41
… contains the epitopes for several of the broadly neutralizing antibodies against HIV-1. In addition to studies of the monomeric peptide, we have developed a new trimeric MPER construct, designated gp41-M-MAT, that associates with detergent micelles and lipid bilayers. This construct is a stable …
-
The Structural Basis of Flaviviridae Interaction with Antibodies and Receptors
… Clearance is dependent upon the presence of neutralizing antibodies that are able to disrupt several stages of this process. Given their fundamental role in the viral life cycle, we sought to determine the structural basis for envelope protein interaction with antibodies and receptors for …
Page 1 of 6