Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"neurosphere"”.
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Isolation, Culture and Cryopreservaion of Neural Stem Cell
… Both GFP (+) and (-) cells could generate neurospheres but the differentiation potential into oligodendrocyte was reduced in eGFP(-) neurospheres. To determine differentiation ability neurosphere from single cell, we selected by clonal selection and cultured for 10 day. Single cell was …
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DLL4 and JAG1 in the Glioblastoma Response to Temozolomide Chemotherapy
… effect of single and combination treatment by neurosphere recovery assay. Results: DLL4 and JAG1 overexpression promotes resistance by increasing the TMZ IC50. Neurosphere formation, recovery, and secondary neurosphere formation is increased following DLL4 and JAG1 overexpression and is …
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Neurobiological effects of food fermentation-derived metabolites for metabolic and mental health
… (hypothalamic and hippocampal) and on primary neurosphere cultures (hippocampal). A panel of SCFA were administered to hippocampal and hypothalamic cell lines and were capable of altering brain-derived neurotrophic factor (BDNF) gene expression. These metabolites were also tested on primary …
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P107 negatively regulates the neural precursor pool by repressing Hes1 transcription
… reporter assay, and finally, using the primary neurosphere assay we showed that removal of Hes1 from p107-/- neurospheres restores the number of neurosphere forming cells to wildtype levels. Our results suggest that p107 represses Hes1 transcription through E2F, and demonstrate that an …
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Neural Stem / Progenitor Cells in the Adult Mouse Hypothalamus
… both self-renewal and differentiation using the neurosphere assay. I show that hypothalamic neurospheres can be propagated in culture for extended periods of time, and that they can differentiate into cells of all three neural lineages. I have also determined the precise location of …
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Olfactory progenitor cell transplantation into the mammalian inner ear
… thesis examines the hypothesis that olfactory neurosphere (ONS) and progenitor cells harvested from the olfactory epithelium represent a viable source of graft material for potential therapeutic applications in the inner ear. Olfactory epithelium represents a unique source of pluripotent cells …
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Generation of cells for cell-replacement therapy: Specification of neural precursors in vivo and in neural stem cell cultures
… to the traditionally and more commonly used neurosphere culture system for expansion of fetal neural stem cells. Unfortunately, our investigations also showed that neither in the neurosphere nor in the NS cell culture system cells with the characteristic of mesDA neuron precursors are …
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Neural Crest Contributions to Dental Pulp Stem/Progenitor Cells and Craniofacial Structures: Alveolar Processes, Tongue and Temporomandibular Joint
… were cultured in a neurogenic medium to assay neurosphere formation and X-gal staining was performed. Wild type mice were also subjected to the same procedures to serve as controls.</p> <p> <strong>Results:</strong> Dental pulp cells were cultured for several months. Our analysis demonstrated …
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Characterisation of neural progenitors from the adult retina and ciliary epithelium
… of a dormant stem/progenitor population. The neurosphere-forming assay is a tool which has allowed scientists to study the behaviour of neural stem/progenitor cells in vitro. Here, I show that cells deriving from the CNR of the adult microphthalmic retina are proliferative and give rise to …
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Neural Stem Cells in Brain Tumor Development
… analysis. Genomic profiling of tumor-derived neurosphere-forming cells from conditional astrocytoma mouse models show prominent dysregulation of genes involved in neurodevelopmental processes and transcriptional regulation, particularly the hox transcription factors, in high-grade …
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Generation of midbrain dopaminergic neurons in vivo and in vitro: the role of Neurogenin2
… of neural stem and progenitor cells, so called neurosphere cultures, that were neurogenic and maintained a ventral midbrain character over several passages. Although the neurospheres did not spontaneously give rise to dopaminergic neurons when differentiated, TH-positive cells were detected when …
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Characterization of Neural Stem Cells Using the Nestin Enhancer
… and negative cells in nestin-GFP tg mice neurosphere. The microarray profile may help to understand the meaning of GFP in the tg neural stem cells and may give cue to isolate neural stem cell from progenitors.
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The effects of complex I deficiency on neurogenesis and white matter development in a mouse model of Leigh Syndrome (LS)
… This finding was independently validated using neurosphere assays. Transcriptional programs and pathways linked to neurogenesis and oligodendrogenesis were significantly downregulated in KO neural progenitors. Although abundance of oligodendrocyte progenitors in the SVZ was not significantly …
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Combined Inhibition of Lysine-Specific Demethylase 1 and Kinase Signaling as a Preclinical Treatment Strategy in Glioblastoma
… The effect on cell viability, proliferation, and neurosphere formation was assessed, and synergy scores were calculated using Bliss synergy models. Kinase signaling was analyzed via western blot analysis, and <em>in vivo</em> efficacy was evaluated in orthotopic xenograft models.</p> <p>LSD1 …
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Differential Angiogenic Capability and Hypoxia Responses in Glioma Stem Cells
… GSCs using short hairpin RNA (shRNA) inhibited neurosphere formation efficiency, indicating a requirement for HIFs in cancer stem cell self-renewal. HIF1a and HIF2a were also necessary for VEGF expression in GSCs, but HIF2a was not required in matched non-stem glioma cells. In vivo experiments …
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Low Reactive Oxygen Species and High Glycolysis In Glioblastoma Stem Cells:Mechanisms and Therapeutic Implications
… lines derived from clinical tissues by typical neurosphere culture system or orthotopic xenografts, and showed that addition of fetal bovine serum to the medium induced an increase of ROS, leading to aberrant differentiation and decreases of stem cell markers such as CD133. We found that …
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Uncovering the role of notch signaling in development of hypothalamic nuclei in vivo and using in vitro microenvironments
… (HVZ). These cells expand and spontaneously form neurospheres which can be manipulated and studied at the level of mRNA or protein expression. We found that acute chemical inhibition of Notch signaling was sufficient to significantly reduce direct downstream Notch signaling Hes1, Hes5, and Hey1. …
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Impact of anti-viral immunity on neural stem/progenitor cell activity and implications for CNS development
… type NSPCs (WT/NSPCs) as well as a decrease in neurosphere growth. IFN restricted cell cycle progression by inhibiting the G1- to S-phase transition. Cell cycle restriction was associated with decreases in the G1–phase specific cyclin E/CDK2 proteins and in pRb phosphorylation at serine 795 …
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The role of the dopaminergic neurotrophin growth/differentiation factor-5 in the developing rat ventral midbrain
… phenotype in neural progenitor cells, neurosphere cultures prepared from embryonic rat VM were established. The effect of the gestational age of the donor VM on the proportion of cell types generated from neurospheres from E12, E13 and E14 VM was examined. Dopaminergic neurons could …
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Integrative Analysis of Omics Data In Adult Glioma and Other Tcga Cancers to Guide Precision Medicine
… intrinsic tumor bulk but not their corresponding neurosphere cells through both transcriptional and protein level analysis using a panel of sphere-forming glioma cultures and their parent GBM samples.FurthermoreI have demonstrated my hypothesis through longitudinal analysis of paired primary and …