Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"neuronal culture"”.
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A novel polymeric microelectrode array for highly parallel, long-term neuronal culture and stimulation
… patterning would be employed to constrain the neuronal culture network connectivity. In order to ensure longterm survival of the cultures, a bottom feeder layer of glial cells would be grown. In addition to high-throughput screening application, the polymeric microelectrode arrays and …
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Development of a novel chip-based platform for long-term neuronal culture, stimulation, and imaging
… a novel, 3D MEA for use in long term study of neuronal cells. These arrays will be cheap and disposable, allowing integration into a fixed stimulation and imaging setup. Neuronal cell activity will be monitored using a Ca2+ sensitive dye that can be visualized via the imaging setup. In the past …
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A general method for three color STED microscopy with one depletion laser : application to primary neuronal culture
Recent advances in the field of optical nanoscopy have equipped molecular neuroscientists with the means to investigate sub-synaptic phenomena with unprecedented spatial resolution. Indeed, early studies employing these methodologies have revealed an extraordinary level of nanoscale spatiotemporal …
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Investigation of Riluzole’s Synaptic Protection Mechanism Through HSF1-BDNF Axis
… a protective role of Riluzole on rat primary neuronal culture which was abolished by a HSF1 inhibitor. Current data together suggest that Riluzole’s synaptic-protective mechanism is highly possible through a HSF1-BDNF axis. The positive outcome from this study will facilitate filling our …
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Morphological characterization of medium spiny neuron development in vitro
… limited by the absence of an in vitro primary neuronal culture system that produced MSNs with mature in vivo-like characteristics. The experiments detailed in this thesis describe the development and characterization of such a system as well as a morphological analysis of MSN development in …
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Inhibition of the Calcium Plateau Following In Vitro Status Epilepticus Prevents the Development of Spontaneous Recurrent Epileptiform Discharges
… and pathophysiologic changes leading to neuronal plasticity. The neuronal plasticity following SE-induced brain injury can initiate epileptogenesis and lead to the ultimate expression of acquired epilepsy (AE), characterized clinically by spontaneous, recurrent seizures. Epileptogenesis …
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The role of gylcoprotein K (gK) in the ocular and neuropathogenesis of herpes simplex virus-type 1 (HSV-1)
… of latency. Following this work, a primary neuronal culture system was established to further define the role that gK played in neuronal infection and transport. Using this system, gK was found to be a critical determinant for neuronal transport and pathology. Since gK deficient viruses …
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The Characterization Of Pb2+ Toxicity In Rat Neural Development: An Assessment Of Pb2+ Effects On The Gaba Shift In Neural Networks And Implications For Learning And Memory Disruption
… qRT-PCR (molecular genetics), brain and primary neuronal culture immunology (immunohistochemical and cellular approaches), physiological cellular components (synaptosomes and protein expression) and finally through learning and memory assessment with GABA mimetic drug manipulations in the intact …
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Transparent neural interfaces for simultaneous electrophysiology and advanced brain imaging
… diseases and its effects on overall neuronal activity. They were integrated into a microfluidic neuronal culture platform to selectively examine the activity-dependent uptake of tau protein at the pre-synapse. Next, the *in vitro* glass-based transparent MEAs were translated into …
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Cytoskeletal changes in SY5Y neuroblastoma cells exposed to acrylamide: an immunocytochemical study
The neuronal cytoskeleton is vital for normal growth and differentiation of the nervous system, as well as for maintenance of the normal intracellular environment. Without it, major functional deficits occur due to interference with cellular transport of membrane components, proteins and …
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Evaluating the Therapeutic Effect of an Hsp90 Inhibitor in Mouse Models of Alzheimer’s Disease
… with 17-AAG treatment after Aβ challenge in neuronal culture models. The functionality of these changes in the synapse was further confirmed by memory improvement in contextual fear conditioning tests. This work implied a potential strategy in using Hsp90 inhibitors to treat AD. Nevertheless, …
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Derivation of next generation research models of Parkinson’s disease
… progression. Although traditional monolayer cell culture models have significantly enhanced our understanding of the disease, their results have not consistently translated into clinical success. These models lack the desired cellular diversity and fail to mimic in vivo physiological conditions. …
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Investigating tau propagation in situ
… focused on human tissue and then developed a neuronal cell model of propagation to enable further investigation. In the first chapter of my thesis, I investigated the association of assembled tau with brain extracellular vesicles (EVs). This association has been documented in AD and has been …
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Elucidating the reversibility of ataxia
… mice are re-capitulated in a primary cerebellar culture and if so, whether they could be rescued by modulating calcium signaling. Morphological and histological analyses revealed that Purkinje cell degeneration is not uniform throughout the cerebellum of KO mice with Purkinje cells from posterior …
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Charakterisierung der Proteinphosphatase 1E (PPM1E) - Lokalisierung und Trunkation in Gehirngewebe und Effekte auf neuronale Morphologie in primärer Neuronenkultur
… Aktinzytoskeletts in dendritischen Spines und neuronalen Dendriten. Der Verlust an dendritischen Spines und Dystrophie von Dendriten kann im AD-beeinträchtigten Gehirn beobachtet werden. Eine negative Regulation der CaMKII, CaMKIV und PAK1 Kinasen könnte potentiell mit diesen frühen …