Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"neural induction"”.
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The effects of BMP4 on different in vitro models for mouse gastrulation and neural induction
… processes, including gastrulation and neural induction. Gastrulation is an event that changes the topology of the embryo, organising a single layer or ball of cells into the three primordial germ layers: the mesoderm, endoderm and ectoderm. In the mouse embryo, gastrulation starts with …
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Investigating the role of Neuronatin in neural development
Neural induction is the process that occurs during embryogenesis whereby ectoderm is converted into neural tissue. However, the cellular and molecular mechanisms that govern this process are still not completely understood. For instance, in Xenopus, calcium signalling is known to play a pivotal …
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Role of Sonic Hedgehog in Development of the Central Nervous System
… of embryonic stem cells as well as adult neural stem/progenitor cells. However, it has not been elucidated how Shh induces neural induction during early development of nervous system. To elucidate the role of Shh in neural induction, we used a pluripotent embryonal carcinoma cell line, P19 …
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New Therapeutic Strategies for Amyotrophic Lateral Sclerosis Treatment
… cell treatment. Here we investigated whether neural induction enhances therapeutic potentials of mesenchymal stem cells (MSCs). We induced neural induction of MSCs by overexpressing Neurogenin1, a proneural transcription factor. Depending on transplantation time, systemic transplantation of …
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A Reductionist Approach to Modeling Human Corticogenesis
… process that involves three major milestones: 1) neural induction and folding of the neuroepithelium, 2) areal patterning and generation of various progenitor types, and 3) corticogenesis. Our current understanding of the molecular and cellular basis of cortical development comes largely from …
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SGK196 controls stem cell fates by promoting the degradation of TGFβ family receptors
… in human embryonic stem cells (hESCs) inhibits neural induction and enhances mesoderm induction, while SGK196 overexpression represses mesoderm and endoderm induction. SGK196 negatively regulates TGFβ family signaling by interacting with and promoting the degradation of TGFβ family receptors. …
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Redefining the Gastrula Organiser in Early Chick Embryo Development
… a complete secondary axis with a regionalised neural tube when grafted to an ectopic location. The node also demonstrates extraordinary regenerative capacity: following surgical ablation, it can completely regenerate, and normal development follows. Previous observations suggest that a …
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Sonic hedgehog induces Motor Neuron Differentiation in P19 Embryonic Carcinoma Cells
… and motor neurons as well as proliferation of neural precursors and control of axon outgrowth. To explore the roles of Shh in neuronal differentiation, we utilized embryonal carcinomal P19 cells as an in vitro model system. We overexpressed Shh in P19 cells and investigated it’s effects on …
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TWIST1: a subtle modulator of neural differentiation and neural tube formation
… epiblast precursor cells through Neurulation. Neural induction can be studied in its main aspects in vitro. However, the process is poorly understood, especially in regard to when and how a cell becomes specified, and then committed, to be a neural cell. It is, on the other hand, well …
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MiRNA influences in mesenchymal stem cell commitment to neuroblast lineage development
… specific microRNAs (miRNAs) are responsible for neural induction, differentiation and fate specification and if they can induce MSCs to commit to a neuroblast and/or mature neuron cell lineage. The selected miRNAs were miR-107, miR-124 and miR-381, which respectively are known to promote …
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Regulation of morphogen signalling during neural patterning in the Xenopus embryo
… of Hedgehog (Hh) and Wnt signals during neural induction and patterning in the Xenopus embryo. We further reveal an essential role of XSufu in the crosstalk of the two pathways, in which β-catenin activates Hh signalling upon overexpression of Gli1, and Gli inhibits Wnt signalling upon …
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Regulation of PAX6: DNA methylation and MicroRNAs
… 2b) and intronic CpG islands (Study 2c). In neural induction of human pluripotent stem cells, PAX6 gene expression is first activated, and then repressed. Using targeted bisulfite sequencing and methylation-specific PCR it is revealed that the PAX6 gene is differentially methylated in a …
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Direct generation of region-specific induced neural Precursors from adult human fibroblasts using non-viral and viral methods
… fibroblasts (aHDFs) can be directly converted to neural precursor cells, and subsequently mature neurons and glia, by the introduction of the defined neural transcriptional factors SOX2 and PAX6. However, the understanding of the underlying gene changes that occur during reprogramming was limited, …
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Developing a stem-cell system of embryonic development organised by a morphogen signaling centre
… limited morphogenesis and have no anterior neural structures present. Here, we incorporated bone morphogenetic protein-4 as a pulse and a spatially restricted signaling center into gastruloid culture to investigate the extent to which they can recapitulate gastrulation-like events in vitro. …
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Multi-scalar mechanisms timing axial progenitor contribution to the vertebrate body axis
… control ones, and that expression of prospective neural plate marker genes is unchanged after removal of the morphological node. A possible reason for this, despite the hypothesised role for Hensen’s node in neural induction and maintenance, is that expression of many ‘node’ marker genes extends …
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Expression of the repressor element-1 silencing transcription factor (REST) is regulated by IGF-I and PKC in human neuroblastoma cells
… resulting in transcriptional repression of the neural-specific genes [Chong et al., 1995; Schoenherr and Anderson, 1995]. The original proposed role for REST was that of a factor responsible for restricting neuronal gene expression to the nervous system by silencing expression of these genes in …
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Transcriptional regulation of human mu-opioid receptor gene: functional characterization of activating and inhibitory transcription factors
… of MOPr in neurons and T-cell receptor induction of MOPr expression in T-lymphocytes. Three different opioid receptors [mu (MOPr), delta (DOPr), and kappa (KOPr)] belonging to the G-protein coupled receptor super-family have been cloned. They are activated by structurallyrelated …
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Anteriore Musterbildung im Wirbeltierembryo - Die Induktion von Vorderhirn und Herz
… der Maus als Quelle für Signale, die eine Neuralplatte mit anteriorer Identität induzieren können, untersucht. Die Initiierung der Vorderhirnanlage beruht im Säuger auf Signalen aus dem anterioren, primitiven Endoderm. Dennoch sind, wie in dieser Arbeit gezeigt werden konnte, Signale aus …