Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 3 of 3 for “"nanoscale apolipoprotein bound bilayers (NABBs)"”.
-
Studies of G-Protein Coupled Receptors Incorporated Into a Novel, Nanoscale, Membrane-Mimetic System
<p>From first principles of phospholipid — apolipoprotein A-I (apo A-I) interactions, we hypothesized that the amino acid sequence of apo A-I derived from a different species may exhibit improved properties compared to human apo A-I for the purpose of incorporation of G protein-coupled receptors …
-
Single Molecule Ligand Binding Studies on CCR5 by Fluorescence Cross-Correlation Spectroscopy
… issue, we developed a novel zebrafish derived apolipoprotein AI (ZapN1) for the assembly of nanoscale apolipoprotein bound bilayers (NABBs). We optimized expression and purification of ZapN1 from E. coli and the assembly of NABBs with different lipids and detergent conditions. We performed FCCS …
-
Studies of G Protein-Coupled Receptor Stability and Dimerization Using Novel Fluorescence and Crosslinking Approaches
… CCR5 into an artificial membrane scaffold called nanoscale apolipoprotein bound bilayers (NABBs). CCR5 was shown to retain proper folding in NABBs and proof-of-concept fluorescence correlation spectroscopy (FCS) experiments were carried out to characterize these structures. Novel FCS standard …