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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 194 for “"nanopore"”.
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Nanopore technology for DNA sequencing
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Electrokinetic phenomena in nanopore transport
Nanopores are apertures of nanometric dimensions in an insulating matrix. They are routinely used to sense and measure properties of single molecules such as DNA. This sensing technique relies on the process of translocation, whereby a molecule in aqueous solution moves through the pore under an …
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Selective molecular transport in nanopore systems
… characterization of the physics of novel nanopore systems has the potential to revolutionize water filtration, nanofluidics, and biomolecule detection technologies. I give my characterizations of five nanopore systems as my dissertation. First, I present my study of nanopores in …
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Nanopore-Based Readout of Encoded DNA Nanostructures
Nanopore sensing is a powerful technique for the detection and study of single molecules. The underlying resistive pulse principle relies on molecules modulating an ionic current as they pass through a nanoscale constriction between two reservoirs. The technique has recently been extended to the …
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Nanopore systems for protein characterization and biomimicry
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01
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Nanopore-based Protein Sensing with DNA Nanostructures
… two decades ago, scientists created the first nanopore- an α-hemolysin channel inserted into a lipid bilayer separating two buffered KCl-filled compartments. Today, nanopores are often associated with sequencing – likely due to the immense success of companies like Oxford Nanopore Technologies. …
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Translocation of a Semiflexible Polymer Through a Nanopore
<p>The transport of a biomolecule through a nanopore occurs in many biological functions such as, DNA or RNA transport across nuclear pores and the translocation of proteins across the eukaryotic endoplasmic reticulum. In addition to the biological processes, it has potential applications in …
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Improving the Signal-to-Noise of Nanopore Sensors
Over the last five years, solid state nanopore technology advanced to rival biological pores as a platform for next generation DNA sequencing. Fabrication improvements led to a reduction in nanopore diameter and membrane thickness, offering high precision sensing. Custom electronics were developed …
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Solid-state nanopore sensors for nucleic acid analysis
Nanopore DNA analysis is an emerging technique that involves electrophoretically driving DNA molecules through a nano-scale pore in solution and monitoring the corresponding change in ionic pore current. This versatile approach permits the label-free, amplification-free analysis of charged polymers …
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Single-Molecule Characterization of RNA via Nanopore Sensing
… followed by characterization via solid-state nanopore sensing. I first describe the nanopore observables and nucleic acid parameters relevant to nanostructure characterization. Then, I demonstrate how tandem implementation of nanostructure engineering and nanopore sensing can be used to size …
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Analysis of Polyethylene Glycol in the α-Hemolysin Nanopore
<p>Nanopores have been shown to be a useful analytical tool for single molecule detection. They have been used to study the composition of DNA and other molecules of interest. These pores are usually α-hemolysin which is a toxin from Staphylococcus aureus or more recently nanoscale synthetic solid …
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Computational studies of semiflexible polymer translocation in nanopore systems
… of double stranded DNA (dsDNA) through nanopore systems is a field with rich physics and many promising technological applications. Double stranded DNA is not fully flexible, and the translocation dynamics of semiflexible molecules are not very well understood. In this thesis, in a set …
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Detection of food contaminants by SERS and nanopore technology
… by single-nucleotide difference facilitated by nanopore technology. The synthetic DNA sequences, which mimic the target of E. coli O157:H7 or other serotypes, were mixed with DNA probes (with or without a locked nucleic acid (LNA) substitution) to form double-strand duplexes. Results show single …
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2D materials based nanopore structures as single molecule sensors
Nanopores are impedance based bio-sensors. The principle of nanopore sensors is analogous to that of a Coulter counter. A nanoscale aperture (the nanopore) is formed in an insulating membrane separating two chambers filled with conductive electrolyte. Charged molecules are driven through the pore …
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Computational study of graphene nanopore sensor for DNA sensing
… to sequence the genome of individuals using nanopore technology can lead to tremendous advancement in the eld of modern medicine. The thickness of the membranes employed in nanopore-based sensors presents a fundamental limitation to the physical dimension, of the translocating DNA molecule, …
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Examining bacterial variation with genome graphs and Nanopore sequencing
… relating to a critical bacterial pathogen: can Nanopore sequencing of Mycobacterium tuberculosis provide high-quality public health information? We collect data from Madagascar, South Africa, and England to help answer this question. First, we assess outbreaks identified using single-reference …
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NANOPORE SEQUENCING AS A NEW TOOL TO EXPLORE COMPLEX TRANSCRIPTOMES
… we leveraged an adaptation of direct-RNA Nanopore sequencing (dRNA-Seq), named NRCeq, to obtain a comprehensive full-length annotation of the SARS-CoV-2 transcriptome. In parallel, we identified putative pseudouridylation sites on multiple sgRNAs, one of which was located in a well-known …
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Stability and bandwidth investigation of alternative structures for nanopore sensors
… the most promising technologies among these is nanopore sequencing. Nanopore sequencing involves the threading of a DNA molecule between two electrolytic reservoirs through a nanometer-sized pore on a synthetic or an organic platform by means of electrophoresis and/or mag- netism. During the …
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Computational modeling of DNA and protein sensing via nanopore systems
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-08-01
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Nanopore/Nanotube Pattern Formation through Focused Ion Beam Guided Anodization
… anodic oxide layers exhibit interesting nanopore/nanotube structures. In this work, focused ion beam patterning method is introduced to general anodization, aiming to make highly ordered anodic porous alumina and titania nanotubes. Focused ion beam guided porous anodic alumina is carried …
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