Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 51 for “"nanobody"”.
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Targeting the N-myc oncoprotein using nanobody technology
… Egr1, leading to p53-independent apoptosis. A nanobody against the bHLH-LZ (Nb C2) was shown to bind both N- and c-myc to similar magnitudes. Its affinity for N-myc bHLH-LZ was superior to that of the small molecule myc inhibitor 10058-F4, which prolongs survival in a MYCN-dependent mouse model …
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Nanobody-Based Interactomic Studies of Single Transcripts During mRNA Maturation
… an improved pipeline for the identification of nanobody repertoires against any antigen of interest, which provided us with 25 nanobodies against GFP, the most common and robust protein tag in use. This pipeline has also allowed us to develop nanobodies against a variety of other antigens of …
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Advancing nanobody discovery and engineering with sequence-based machine learning
… antibodies. Since the approval of the first nanobody-based drug in 2019, they have gained significant momentum as therapeutics. Yet the development of these biologics remains a challenge. While in vitro directed evolution technologies are relatively fast and cheap to deploy, and in silico …
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Protein glycosylation by NleB and the secretion of therapeutic nanobody fusions
… an engineered T3SS was created to secrete the nanobody fusions, and in vivo analyses before and after precipitation were completed using C. rodentium and E. coli. Nanobodies fused with effector signal sequences upstream from the camelid signal sequences were expressed, but not secreted through …
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Using data to characterise the relationship between nanobody sequence, structure and function
Nanobodies (Nbs) are a class of single-domain antibody derived from the immune systems of camelid species. They achieve binding affinities and specificities to target antigens comparable to those of classical antibodies (Abs), despite being ten times smaller (~15 kDa) and having only three variable …
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Selective Degradation of Aggregated Tau Protein Via Vectored Nanobody-TRIM21 RING Fusion Constructs
… tau. These TRIMinators consist of an anti-tau nanobody fused to the E3 ubiquitin ligase domain of TRIM21. This E3 ligase activity is initiated by intermolecular clustering, which was hypothesised to allow for the selective degradation of multivalent aggregated tau:TRIMinator complexes whilst …
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Developing a nanobody based technique to investigate interactions between signalling proteins in intact cells
Abstract not available. Please refer to PDF.
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Specific cellular labeling of nuclear and membrane proteins with antibody alternatives: Affimer and nanobody
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-12-01
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A nanobody suite for yeast scaffold nucleoporins provides details of the Y complex structure and nuclear pore complex assembly
… We mapped the epitope of eight members of the nanobody library via crystal structures of nup-nanobody co-complexes.
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Engineering Optogenetic Control of Endocytic Recycling: Controlling Rab11 Function in Drosophila melanogaster using Engineered Light-Responsive Nanobodies
… a genomically non-invasive construct (Opto-Nanobody) utilizing an optogenetic, light-sensitive Cryptochrome 2 (Cry2) fused to YFP-targeting nanobodies to bind functional, endogenous, YFP-tagged Rab11. This system promises to provide precise light-responsive spatio-temporal control of Rab11 …
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Transdermal delivery of heavy chain only antibody binding domains using hydrogel-forming microarray patches
… heavy chain only antibodies (HCAbs) such as Nanobody® molecules and VNARs, derived from camelids and sharks, respectively. Owing to their small size (~13 kDa) and reduced complexity, they are less expensive to produce than mAbs. Furthermore, their potency has been shown to be an order of …
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Developing nanobodies to stabilise the tumour suppressor protein p16INK4a
… The single variable domain, known as a nanobody, is, at 15 kDa, the smallest antigen binding fragment, a tenth the size of a standard IgG antibody. The small size and relative ease of production, coupled with an unusually high stability, makes nanobodies useful tools as biological …
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Sélection et caractérisation de Nanobodies inhibiteurs de métallo-β-lactamases
… d’identifier un Nb inhibiteur de VIM-4, i.e. le Nanobody Nb_VIM38. Ce Nb a été fusionné à la protéine « cherry » pour augmenter sa production. Des concentrations de l’ordre du μM en cherry-NbVIM_38 inhibent l’hydrolyse de toutes les β-lactamines testées. Par ailleurs, un modèle d’inhibition mixte …
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Engineering protein components for living electronics
… ferredoxin through insertion of an anti-GFP nanobody. I demonstrate that a ferredoxin which contains a specific anti-GFP nanobody insert requires co-expression with GFP to display electron transfer activity. These studies lay the groundwork for further development of biological components for …
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Synthesis and characterisation of peptide-based probes for quantitative multicolour STORM imaging
… native chemical ligation. The labelled anti-GFP nanobody was to demonstrate ensemble and single molecule imaging in S. pombe, as well as characterisation on single molecule surfaces in comparison to a conventional randomly labelled antibody. The stoichiometrically labelled nanobody had a more …
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Expression of Nanobodies in Marchantia polymorpha chloroplasts
… finally attempted to produce an anti-mCherry nanobody tagged with mTurquoise2 (LaM4) through nuclear and chloroplast expression in Marchantia (Chapter 5). For expression of transgenes from the Marchantia nuclear genome, I tested different promoters and subcellular locations of protein …
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Applications of yeast display in synthetic biology with a focus on biological synthesis and recycling of plastics
… as beads. As a proof of concept, an anti-GFP nanobody was used to capture GFP from bacterial lysates and GFP-tagged LNX1 protein from mammalian cell lysates. Co-precipitation of the LNX1 binding partners liprin-α1 and Numb, showed that yeast display can be used for studying protein-protein …
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Interrogation and Manipulation of the IGG FC Glycan
… sialic acid residues. Solving the structure of a nanobody-Fc complex via x-ray crystallography reveals a unique mode of recognition of IgG glycoforms. By adapting nanobodies to standard biochemical methods, I clinically stratify dengue virus and SARS-CoV-2 infected individuals based on their Fc …
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Metal Mediated Mechanisms of Drug Release
… drug doxorubicin to a tumour targeted anti-HER2 nanobody. The drug could then be delivered to cancer cells upon addition of a palladium complex. The second project relates to the development of a platinum mediated bond-cleavage reaction. This was developed with the aim of using …
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Investigating the role of ICL3 in β1-adrenergic receptor activation
… β1ARhICL3 bound to antagonist and inactivating nanobody, active state bound to agonist and active-state nanobody, a second active state bound to miniG, and a SH3 domain bound state are observed. Chapter 5 discusses the biophysical characterisation of the β1ARhICL3 where the dissociation …
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