Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"nanobodies"”.
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Expression of Nanobodies in Marchantia polymorpha chloroplasts
… of recombinant proteins, such as antibodies and nanobodies, is essential for biomedical and other practical applications. Plants possess many advantages for recombinant protein production, including lower costs, larger scale production compared to other systems and low risk of contamination by …
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Developing nanobodies to stabilise the tumour suppressor protein p16INK4a
… coupled with an unusually high stability, makes nanobodies useful tools as biological reagents, crystallography chaperones and therapeutics. The research contained within this PhD looks at the development of nanobodies to target p16. By leveraging the high stability of selected nanobodies, the …
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Sélection et caractérisation de Nanobodies inhibiteurs de métallo-β-lactamases
… nous avons voulu explorer le potentiel des Nanobodies (Nbs) de camélidé en tant qu’inhibiteurs de β-lactamases, et plus particulièrement de MβLs. Ces fragments d’anticorps correspondent aux domaines variables des anticorps à chaîne lourde produits par les camélidés (HCAbs). Aussi appelés …
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Development of nanobodies as tools to investigate G-quadruplex DNA secondary structures
… BG4. A phage-display screen was used to select nanobodies, antibody fragments derived from of heavy-chain only antibodies of Camelid family, that have high affinity and selectivity for the G4 structure from the human *MYC* oncogene promoter (MycG4). Biophysical characterisation confirmed that …
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Study of a Transferrin Receptor Brain Shuttle for the Delivery of Nanobodies Against GABAA Receptors
… the delivery of novel immunogenically-raised nanobodies (Nbs), which exhibit promising subtype-selectivity and pharmacological activity against neuroinhibitory γ-aminobutyric acid type A receptors (GABAARs). Initially, anti-GABAAR Nbs were genetically fused to the BBB shuttle, 8D3, and the …
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Engineering Optogenetic Control of Endocytic Recycling: Controlling Rab11 Function in Drosophila melanogaster using Engineered Light-Responsive Nanobodies
… Cryptochrome 2 (Cry2) fused to YFP-targeting nanobodies to bind functional, endogenous, YFP-tagged Rab11. This system promises to provide precise light-responsive spatio-temporal control of Rab11 function in response to blue-light exposure through homo-oligomeric clustering, which has been …
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Advancing nanobody discovery and engineering with sequence-based machine learning
Nanobodies, also known as single-domain antibodies, are small antibody fragments naturally produced in camelids. Notable for their high expressibility, solubility, and stability, they exhibit binding affinities comparable to those of conventional antibodies. Since the approval of the first …
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A nanobody suite for yeast scaffold nucleoporins provides details of the Y complex structure and nuclear pore complex assembly
… subcomplexes, we developed a suite of twelve nanobodies that recognize seven constituent nucleoporins of the Y and Nic96 complexes. The nanobodies bind their targets specifically and with high affinity, albeit with varying kinetics. We mapped the epitope of eight members of the nanobody …
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Nanobody-Based Interactomic Studies of Single Transcripts During mRNA Maturation
… of traditional antibodies, we chose to use nanobodies: recombinant single domain derivatives of a heavy chain-only antibody variant found in camelids. The recombinant nature and small size of nanobodies make them ideal reagents for affinity isolations. We developed an improved pipeline for …
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Synthesis and characterisation of peptide-based probes for quantitative multicolour STORM imaging
… attached to expressed anti-GFP and ant-mCherry nanobodies via native chemical ligation. The labelled anti-GFP nanobody was to demonstrate ensemble and single molecule imaging in S. pombe, as well as characterisation on single molecule surfaces in comparison to a conventional randomly labelled …
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Targeting the N-myc oncoprotein using nanobody technology
… cancers such as MYCN-amplified neuroblastoma. Nanobodies, camelid-derived single-domain antibodies, are a relatively new drug class. Whilst some are already in clinical trials for a wide range of diseases, these are specific for cell-surface or extracellular targets. However, their properties …
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Antibody-Effector Functions in Antiviral Response
… the discovery of a novel class of engineered nanobodies which can discriminate between IgG glycoforms. Using this platform, I define two classes of nanobodies, one which can recognize IgG lacking its core-fucose and one which can recognize IgG bearing terminal sialic acid residues. Structural …
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Interrogation and Manipulation of the IGG FC Glycan
… thesis, I identify a novel class of synthetic nanobodies that can distinguish glycoforms without reactivity to off-target glycoproteins or glycans. Applying this technology to Fc glycoforms defines nanobodies that specifically recognize either IgG lacking its core-fucose or IgG bearing terminal …
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Cryo-EM structural exploration of human VPS34 complexes and their activation by RAB GTPases and NRBF2
… the binding sites of eight anti-VPS34 complex nanobodies. The structural information explains the differential functional effects some nanobodies have on complexes I and II. The nanobodies can be used to label VPS34 complexes in in vitro and in vivo experiments, and the structural data can …
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Protein glycosylation by NleB and the secretion of therapeutic nanobody fusions
… instead of secreting a whole effector, camelid nanobodies were fused with signal sequences 20 amino acids in length from effectors that are naturally secreted and examined. An attenuated bacterium with an engineered T3SS was created to secrete the nanobody fusions, and in vivo analyses before …
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Production, characterization and evaluation of the chemotherapeutic and immunogenic potential of protein based virus-like particles in tumoral and immune cell lines
… technology was also used to conjugate anti-EGFR nanobodies to the surface of DOX-loaded VLPs. It is shown that particles displaying anti-EGFR nanobodies promote a more accentuated decrease on cell viability of human colorectal cancer cells whether compared with non-decorated particles. In …
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Microfluidics and chemical kinetics to analyse protein interactions, aggregation, and physicochemical properties
… I investigate the inhibiting effect of llama nanobodies on Aβ(1-42) aggregation. The findings from this study show that nanobodies target monomeric species with high affinity whereas interactions with fibril surfaces are weak. Finally, I discuss the use of other compounds inhibiting specific …
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Applications of yeast display in synthetic biology with a focus on biological synthesis and recycling of plastics
… library was successfully screened to select for nanobodies that bind the target proteins (small molecule binders Dig10.2 and FDCA decarboxylase) the disruption of the nanobody-protein interactions by the small molecule analytes has not yet been demonstrated. However, nanobodies that specifically …
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Engineering protein components for living electronics
… produced demonstrates that insertion of nanobodies is a viable strategy for controlling protein activity through specific protein-protein interactions. Further successful application of this strategy has the potential to greatly expand our ability to regulate protein electron transfer for …
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