Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"naive pluripotency"”.
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Determining the Signalling Pathways that Govern Human Naive Pluripotency
… and occupy a developmentally advanced state of pluripotency comparable to mouse EpiSCs. Recent reports demonstrate that a naïve state of human pluripotency can be consistently derived either through transient histone deacetylase inhibition mediated resetting of conventional hESCs or via …
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Understanding the Dynamics of Embryonic Stem Cell Differentiation
… embryonic stem (ES) cells are self-renewal and naive pluripotency, the ability to give rise to all cell lineages in the adult body. In addition to being a unique and interesting cell type, pluripotent ES cells have demonstrated their potential for continued advancements in biomedical science. …
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The NuRD complex regulates chromatin dynamics driving exit from naïve pluripotency
… (NuRD) complex is essential for exit from naive pluripotency both in mammalian embryonic stem cell (ESC) culture and in vivo. However, the specific mechanisms of chromatin modulation by NuRD in this process have not been fully characterised. This study used rapid, auxin-inducible removal of …
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Genetic Dissection of the Exit of Pluripotency in Mouse Embryonic Stem cells by CRISPR-based Screening
The ground state naive pluripotency is established in the epiblast of the blastocyst and can be captured by culturing mouse embryonic stem cells (mESCs) with MEK and GSK3 inhibitors (2i). The transcription network that maintains pluripotency has been extensively studied with the indispensable core …
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The role of mechanotransduction in signalling and pluripotent stem cell fate
… factors of the extracellular matrix (ECM) affect pluripotency and cell fate will not only improve the differentiation of pluripotent stem cells in vitro, but also advance the understanding of development. In this study, we used StemBond, a hydrogel chemistry in which stiffness and ECM-to-gel …
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… embryology for decades. One of those aspects is pluripotency, the ability of embryonic cells of certain developmental stages to divide indefinitely without the loss of their potential to differentiate into derivatives of the three primary germ layers. Pluripotency has been captured in vitro by …
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Single-molecule microscopy reveals details of heterochromatin reorganisation at the onset of mouse ES cell differentiation
… to address these questions, since upon exit from naive pluripotency their genome undergoes a dramatic structural reorganisation. The development of new fluorophores and imaging technologies has enabled researchers to observe single biological molecules, in both fixed and live cells. These …
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Mechanisms of Oct4 in the entry to, maintenance of, and exit from pluripotency
Pluripotency is defined as the capacity to give rise to all cell types of the embryo proper. It arises in the early mammalian embryo but is lost after a short period of time as cells differentiate and become committed to different lineages. Prior to implantation, mouse epiblast cells enter the …