Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 22 for “"myristoylation"”.
-
Characterization and molecular cloning of sos3: A gene important for salt tolerance and potassium nutrition in higher plants
… EF-hand calcium binding domains and a putative myristoylation motif at its NH₂-terminus. SOS3 binds calcium and is myristoylated in vitro. A mutation in SOS3 that destroys the conserved myristoylation motif abolishes SOS3 myristoylation, but not its calcium binding in vitro. Furthermore, the …
-
Calcium -dependent protein kinases are myristoylated and associated with different membranes in Arabidopsis thaliana
… 28 of the predicted CDPK proteins have potential myristoylation motifs at their amino termini. Myristate is a 14-carbon saturated fatty acid that is attached co-translationally to the amino-terminal glycine of a nascent protein. Myristoylation can facilitate membrane binding and/or protein-protein …
-
AMPK Regulation of Myeloid Inflammatory Responses During Acute and Chronic Disease
… we interrogate the effects of AMPK β subunit myristoylation and how it affects AMPK protein stability. We measured AMPK protein levels in cells and mice lacking the AMPK myristoylation site (β1/β2-G2A) and observed a strong decrease in steady state 𝛾 subunit levels alongside more mild …
-
Structural and Functional Characterization of GAPR-1, a Mammalian Plant Pathogenesis-related Protein in Lipid-enriched Microdomains of the Golgi Complex
… identified. It contains a consensus sequence for myristoylation, a putative caveolin-binding domain, a coiled-coil structure, and an isolelectric point (pI) of 9.4, suggesting that GAPR-1 is a highly hydrophilic protein (Eberle, H. B. et al. 2002). In this thesis, this structural information, was …
-
Assessment of <i>N</i>-myristoyltransferase and the <i>N</i>-myristoylomeas a potential chemotherapeutic target in<i>Trypanosoma cruzi</i>
… of the epimastigote. While both NMT and <i>N</i>-myristoylationwere detected in all lifecycle stages, there were subtle differences in theexpression of several myristoylated proteins. However, at least ~10 myristoylatedproteins were common throughout the lifecycle. In addition, …
-
Develoment and Application of Chemical Strategies to Study Protein Fatty-Acylation in Eukaryotes
… and palmitoylated proteins respectively. Since myristoylation is co-translational and constitutive, I employed both palmitoylation and myristoylation chemical reporters with orthogonal fluorophores to simultaneously monitor palmitate and protein turnover. Dual pulse-chase analysis of Lck, a …
-
A Phosphoinositide-Specific Phospholipase C of Trypanosoma Cruzi: Unique Characteristics of a Delta-Type Homologue
… revealed some unique features such as an N-myristoylation consensus sequence at its N-terminal end, lack of an apparent PH domain and a highly charged linker region between the catalytic X and Y domains. TcPI-PLC is lipid modified in vivo as demonstrated by metabolic labeling with …
-
Feedback Regulation of Wnt Signaling by Naked Cuticle (Nkd) During Drosophila Embryogenesis
… mammalian Nkd homologs that have N-terminal myristoylation consensus sequences responsible for membrane association, the N-terminus of Drosophila Nkd, also conserved in mosquito Nkd, lack such a sequence. Nonetheless, Nkd's N-terminus was required for function and membrane association. …
-
The characterisation of calpain-like proteins in Trypanosoma brucei
… calpain-like proteins contain N-terminal dual myristoylation/palmitoylation signals, a protein modification involved in protein-membrane associations. We analysed the expression of calpain-like proteins in the insect (procyclic) and bloodstream-stage of T. brucei using quantitative real time …
-
Protein Dynamics: Studies of Adenylate Kinase Mutants From Escherichia Coli and Characterization of Adenylate Kinase Isoforms From Murine Cells. Applications of Fluorescence Spectroscopy and Microscopy
… affinity to the GUVs. This result suggested that myristoylation of AK1beta played an important role in its binding to the GUVs.
-
Lysine Polyphosphate Modification Regulates Enzymatic and Signaling Functions in Eukaryotic and Bacterial Systems
… functional analysis of a selected KPM target, N-myristoylation transferase 2 (NMT2). Using a fluorescence-based enzymatic assay, we observed that KPM of NMT2 reduces the release of myristoyl groups, consistent with partial inhibition of its catalytic activity. These results provide functional …
-
Trypanosoma Cruzi Phosphoinositide -Specific Phospholipase C (tcpi-Plc) and Its Role in Differentiation
… deletion mutants fused to GFP, we found that myristoylation of a glycine residue in the second position, and acyl modification of cysteines in the fourth, eighth, and fifteenth positions of the coding sequence, are required for correct plasma membrane localization. TcPI-PLC is translocated …
-
Spatiotemporal Regulation of the NADP(H) Phosphatase Nocturnin
… and other membranes via N-terminal glycine myristoylation. In contrast, mitochondrial NOC levels are highly circadian with peak expression during the early dark phase. Overall, our work suggests that NOC links circadian clocks to metabolism by regulating local intracellular concentrations of …
-
Trimming Fat Upon Infection: Proteolytic Demyristoylation as a Novel Bacterial Pathogenic Strategy
… of IpaJ as a research tool to study protein N-myristoylation and the mechanisms of membrane trafficking.
-
Identification of TbRP2-interacting proteins using proximity-dependent biotinylation (BioID)
… the consensus sequence specifying N terminal myristoylation (a modification that targets XRP2 to the basal body in mammalian cells) but rather utilises twinned TOF-LisH motifs at the Nterminus of the protein to direct basal body targeting. To further interrogate the targeting and function of …
-
3-Hydroxy-3-methylglutaryl-coenzyme A lyase: investigation of cysteines mediating intersubunit disulfide formation and regulation by thiol/disulfide exchange and discovery of an extramitochondrial homolog
… A lyase. In-vitro myristoylation experiments confirm that the protein is modified by Nmyristoyltransferase and immunofluorescence microscopy in COSI cells demonstrate that this modification effects the subcellular localization of the protein. Blots of rodent organ …
-
Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
… microdomains is neither influenced by SSP myristoylation nor by the co-expression of the matrix protein do Z. Regions of plasma membrane containing Z not co-localize with GP-C containing microdomains. Clustering of proteins or lipids on the plasma membrane may bring Z and GP-C together at …
-
Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
… microdomains is neither influenced by SSP myristoylation nor by the co-expression of the matrix protein do Z. Regions of plasma membrane containing Z not co-localize with GP-C containing microdomains. Clustering of proteins or lipids on the plasma membrane may bring Z and GP-C together at …
-
DHHC PROTEIN ACYLTRANSFERASES CATALYZES THE LYSINE PALMITOYLATION OF MEMBERS OF THE RAS SUPERFAMILY OF SMALL GTPASES
… class, followed closely by N-terminal glycine myristoylation and lastly by O-serine fatty acylation. In recent years, a previously underappreciated member of this class, lysine N ε - fatty acylation, has gained traction due to work by Jiang et al with their discovery that SIRT6 can …
-
Deciphering host-parasite molecular networks mediating resistance to toxoplasma gondii
… In addition, one of the GIMAPs contained an N-myristoylation motif that is important for protein–protein/lipid interaction and plays an essential role in membrane targeting. Importantly, we found that all those GIMAPs possess an LC3-interacting region (LIR) that is important for recruitment of …
Page 1 of 2