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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 64 for “"myosin heavy chain"”.
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Gene Expression of myosin heavy chain isoforms in pigs of different genetic groups and ages
… study was to evaluate gene expression pattern of myosin heavy chain isoforms in pigs of three different genetic groups at four ages. 48 castrated males of Piau, Commercial and Crossbred (Piau X Commercial) genetic group, slaughtered at birth, 56, 112 and 156 days. Longissimus dorsi muscle samples …
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Metabolic profile of myosin heavy chain-based fiber types in the rat soleus after spinal cord transection
… enzymes) and types of contractile proteins (myosin heavy chain, MHC) that are expressed. Research has shown that decreased neuromuscular activation following spinal cord transection (ST) results in adaptations in the physiological characteristics of paralyzed muscles, including atrophy and an …
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Expression der Isoformen des „Myosin Heavy Chain“ (neonatal, developmental und fast) sowie N-CAM bei myogenen und neuromuskulären Erkrankungen
… dabei in den letzten Jahren auch die Gruppe der Myosin Heavy Chains (MHCs), Hauptbestandteil der dicken Filamente im Sarkomer. <br>Mittels Immunhistochemie konnten bislang sieben Isoformen dieser Proteinfamilie entdeckt werden, die im Skelettmuskel vorkommen. Dies sind vier „fast“ Isoformen …
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A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus
The overall aim of this study was to contribute knowledge to two areas of inquiry in muscle research: one concerned with the molecular mechanism(s) underlying the positive correlation between intracellular glycogen content and skeletal muscle performance and the other with the MHC isoform …
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Purification of Two Myosin -Degrading Proteases From Porcine Skeletal Muscle
Myosin is the major skeletal muscle protein. However, the protease system responsible for myosin degradation in vivo has not yet been clearly described. Two myosin degrading proteases, a serine protease and a cysteine protease that might have some function in myosin degradation, were isolated from …
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Role of the Sh3 and Cysteine-Rich Domain 3 (STAC3) Gene in Proliferation and Differentiation of Bovine Satellite Cells
… the expression levels of myogenin and myosin heavy chain genes, and by quantifying fusion index. STAC3 knockdown stimulated mRNA and protein expression of myogenin, and myosin heavy chain 3 and 7, and increased fusion index of bovine satellite cells. These data together suggest that …
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Transcriptional Regulation of Muscle Contractility and Metabolism by a MicroRNA-Mediated Feed Forward Loop
… Messenger RNA transcripts coding for three myosin heavy chain contractile proteins, Myh6, Myh7, and Myh7b, encode microRNAs miR-208a, miR-208b, and miR-499 respectively, within their introns. Here, I demonstrate through gain and loss of function studies in vivo that this family of microRNAs, …
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Delimitating Species Boundaries within the Liomys Pictus- Spectabilis Complex (Rodentia: Heteromyidae)
… introns from engrailed protein (190 bp) and myosin heavy chain (254 bp) genes for approximately 712 individuals representing 82 localities across the range of L. pictus and L. spectabilis. Maximum parsimony, maximum likelihood, and Bayesian analyses suggest that there are six species within …
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Effect of Zilpaterol hydrochloride and steroid implantation on yearling steer feedlot performance, carcass characteristics, and skeletal muscle gene expression
… the receptors for ZH as well as the abundance of myosin heavy chain (MHC) mRNA in skeletal muscle of feedlot steers. It was also determined that ZH can cause a transition in the abundance of MHC mRNA isoforms in skeletal muscle that are available for the translation of larger, faster, more …
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Hypercontraction and Drosophila : a model system for the study of human myopathies
… induced myopathy caused by mutations in Myosin Heavy Chain has led to a potential mechanism of hypercontraction through unregulated contraction cycles in mutant muscles. In addition, hypercontraction defects cause temperature-sensitive myogenic seizures due to an altered state of the …
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A study of rat skeletal muscle Troponin C isoforms
… part of this study, was used in conjunction with myosin heavy chain (MHC) isoform based fibre-typing and Sr2+ -activation measurements to explore the relationship between MHC and TnC isoform composition in mammalian skeletal muscle at the single fibre level, and to revisit the controversial issue …
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Proteomic analysis of diurnal variation in human skeletal muscle performance
… of human muscle based on its profile of myosin heavy chain isoforms is commonly used to help understand changes in muscle function. However, in many instances, measureable changes in force output or contractility occur in the absence of any change in myosin heavy chain profile. Therefore, …
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A study of biochemical and physiological properties of normal and functionally impaired skeletal muscles
… gel electrophoresis (SDS-PAGE) analyses of Myosin Heavy Chain (MHC) isoform composition in single fibres and whole muscle homogenates] and physiological (single fibre measurements of contractile activation characteristics and caffeine thresholds for contraction) methods. From the beginning …
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Effects of the MYH7 R369Q Dilated Cardiomyopathy-causing Mutation on Myosin Crossbridge Kinetics and Cardiomyocyte Contractility
… (DCM). This mutation is located in loop 4 of β-myosin heavy chain actin-binding surface and may alter actin-myosin interaction that facilitates cardiomyocyte contraction. There are limited insights into the nature and implications of this mutation on the contractile system within a cardiomyocyte …
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Investigation of the mechanisms underlying the effects of hyperglycaemia on cardiac structural and electrical remodelling
… calcium ATPase 2 (SERCA 2) and α- and β-myosin heavy chain. Hyperglycaemia decreased the number of beating EBs, their beating rate, and their amplitude of contraction. It also decreased the calcium transient amplitude and the contractile response to ryanodine receptor stimulation by …
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Fiber Type-specific Desmin Content in Human Single Muscle Fibers
… sample and analyzed using SDS-PAGE to determine myosin heavy chain (MHC) composition. Following identification, muscle fibers were pooled into two groups (MHC I and MHC IIa). Desmin and actin content within the pooled samples was determined via immunoblotting. On average, muscle samples were …
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Role of JAK3 in Myogenic Differentiation
… multinucleated myotubes and the expressions of myosin heavy chain (MHC), myogenin (MGN), MyoD, and myogenic enhancer factor 2 (MEF2). Knockdown of the JAK3 gene using siJAK3 also significantly increased MHC, MGN and MyoD mRNA expressions as well as insulin-like growth factor-II (IGF-II) gene …
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Molecular Mechanisms of Vascular Disease In Patients With Rare Variants In Myh11
… encoding the smooth muscle specific myosin heavy chain, are a rare cause of inherited TAAD. However, rare but recurrent non-synonymous variants in <em>MYH11</em> are present in the general population but do not cause inherited TAAD. The goal of this study was to assess the potential …
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Behaviour of human myoblasts in vitro: role of ageing and inflammatory cytokines
… differentiating muscle cells were myogenin and myosin heavy chain. Additional parameters observed were DNA damage and analysis of specific cytokines in the cell secretome. No differences in any parameter measured were found between cells of young and old people. However senescent myoblasts …
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Role of MicroRNAs in Human Skeletal Muscle Tissue Engineering In Vitro
… 3D constructs led to decreased presence of slow myosin heavy chain and increased fast myosin heavy chain. Finally, co-culture of functional human skeletal muscle with human cord blood-derived endothelial cells is possible in 3.3% FBS in DMEM culture conditions, with significant increases in force …
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