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Showing 1 to 9 of 9 for “"myosin VI"”.
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Single-Molecule Fluorescence Studies of ReAsH and Myosin VI
… eGFP, indicating that ReAsH can be used for in vivo tracking and localization of specific proteins. We also have used FIONA to study myosin VI, the only myosin to move in a direction opposite that of other myosins. This reverse directionality enables myosin VI to be involved in endocytosis. We …
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Single-molecule studies of unconventional motor protein myosin VI
Myosin VI is one of the myosin superfamily members that are actin-based molecular motors. It has received special attention due to its distinct features as compared to other myosins, such as its opposite directionality and a much larger step size than expected given the length of its “leg”. This …
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Studies of myosin mechanics using fluorescence
Molecular fluorescence provides scientists with a wealth of information on how biological systems function at the molecular level. Through fluorescence, individual biological molecules can be tracked as they interact with other proteins, DNA, ligands, etc. and change their conformation in response …
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INVESTIGATING ALTERNATIVE SPLICING REPROGRAMMING REGULATED BY THE E-CADHERIN/B-CATENIN AXIS
Alternative splicing (AS) is vital for proteome flexibility and its aberrations are key drivers of cancer progression. We identified an alternatively spliced exon cassette in myosin VI, an actin motor protein. In healthy epithelial tissues like the ovary and intestine, this exon cassette is …
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Fluorescence Imaging With One Nanometer Accuracy: Determination of How Processive Motors Walk
… without dissociating from the cytoskeleton. Myosin V, myosin VI, and kinesin are well known processive motors. Structurally, these motors are dimeric having two motor heads, two legs, and a common stalk. Whether they move by a ""hand-over-hand"" or an ""inchworm"" model has been a …
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Computational investigations of protein dynamics and its implications for biological functions
… this pressure-jump induced fast folding behavior, MD simulations of more than 35 μs are carried out on the λ*YG mutant. High-pressure denatured states are found to contain a significant amount of helical structure. Upon pressure drop, the protein refolds into the native state in 20 μs. The …
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Studying Processive Molecular Motors Inside Live Cells
… 1 nm (with half-a-second temporal resolution) in vitro. This new localization technique is named Fluorescence Imaging with One Nanometer Accuracy (FIONA). Yildiz et al. has shown that FIONA is precise enough to resolve the hand-over-hand steps of the processive molecular motors myosin V, kinesin …
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Regulatory mechanisms of MYO6 motor domain activity and tail interactions
The myosin super family represent a diverse group of actin-based molecular motors, driven by ATP hydrolysis. They are fundamental for a multitude of cellular processes, ranging from muscle contraction to intracellular transport to tethering. Originating from an ancestral protein, myosins have …
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Specialization of a calmodulin-like protein: Androcam adopts a single conformation over the entire physiological range of calcium
… 'Insert2' peptide of the biological target Myosin VI with its C lobe only, whereas calmodulin binds this target using both lobes. Our results indicate that the androcam sequence has been optimized by evolution starting from the highly versatile calmodulin sequence to adopt only one of the …