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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"myofibrils"”.
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Equilibrium Dialysis Studies of Purine and Pyrimidine Binding to Rabbit Myofibrils
Made available in DSpace on 2014-12-05T20:55:38Z (GMT). No. of bitstreams: 1 5805447.pdf: 2201465 bytes, checksum: af98da1dc92c66e90077493b47db502e (MD5) Previous issue date: 1958
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The rigor bond’s interaction with protein denaturation : effects on water-holding of myofibrils
… bonds’ influence on rheological features of myofibrils in suspension. In this study the rigor bonds’ influences on water-holding capacity were compared; firstly, by dissociating the rigor bonds in one group with the addition of 10mM PPi and 2mM MgCl2; secondly, applying heat treatment at 21ºC …
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The effect of calcium activated protease (CAF) and cathepsin D on bovine muscle myofibrils under varying conditions of pH and temperature
… cardiac muscle) was incubated with isolated myofibrils under near optimal conditions (pH 7.5 and 25(DEGREES)C), a rapid and significant release of soluble protein, including intact alpha-actinin, from the myofibrils was observed. SDS-PAGE analysis of treated sedimented myofibrils showed CAF …
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Purification and some properties of an alkaline protease from rat skeletal muscle
… purification procedure. Incubation of washed myofibrils in the presence of sodium pyrophosphate released a factor into the supernatant, the removal of which facilitated the separation of myofibrillar alkaline protease from the myofibrils. The factor appeared to be necessary for binding of the …
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Contractile Properties of Striated Muscle in Development and In Disease
… that are close to those of fetal cardiac myofibrils and not adult cardiac myofibrils. The human induced pluripotent stem cell derived cardiomyocytes from patients with known mutations show potentially early onset factors in cardiomyopathy. Finally, failing adult cardiac muscle demonstrates …
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Effects of the MYH7 R369Q Dilated Cardiomyopathy-causing Mutation on Myosin Crossbridge Kinetics and Cardiomyocyte Contractility
… The rate of ATP binding to isolated myofibrils, as measured using the stopped-flow technique, was observed to be slower for the R369Q myosin variant. As contractility is driven by transient rise of intracellular calcium, there is no significant difference between the calcium transient …
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Comparison of thermal gelation properties of pork, beef, fish, chicken and turkey
… muscles (10% protein at pH 6.0) and myofibrils (8% protein at pH 6.0) were evaluated.
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The Role of MCL-1 in the Heart: Gateway from Life to Death
… dilated cardiomyopathy preceded by loss of myofibrils and cardiac contractility, abnormal mitochondria ultrastructure, defective mitochondrial respiration, and impaired autophagy. Genetic ablation of both pro-apoptotic effectors (Bax and Bak) could largely rescue the lethality and impaired …
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Identification and characterisation of novel marker proteins involved in X-linked muscular dystrophy
… stress proteins was observed within dystrophic myofibrils compared to normal fibres. This thesis has successfully identified novel biomarkers that may be used to determine suitability of new treatments or therapies of muscular dystrophy.
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Integrin Alpha Subunit Ratios, Cytoplasmic Domains, and Growth Factor Synergy Regulate Muscle Proliferation, Differentiation, and Signaling
… muscle $\alpha$-actinin organization into myofibrils. bFGF and insulin promote survival of $\alpha$5 transfected myoblasts in serum-free medium. TGF-$\alpha$ and TGF-$\beta$ promote survival of untransfected myoblasts. Our observations demonstrate: (1) a specificity for integrin $\alpha$ …
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Probing the effect of microstructure on water diffusion in engineered or biological materials with MRI
… used to mimic the effect of the organization of myofibrils on water transport. Finally, the extraction of mass transfer properties from MRI data requires the integration of the underlying mass diffusion process with MRI imaging physics. In this thesis, the diffusion-weighted MRI protocol is used …
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Lattice Boltzmann method for integrating the Bloch equation in muscle fibers and microvessels
… to include an ordered or disordered array of myofibrils and lipid domains inside the muscle fiber, and numerically simulate the multiphase transport of water, certain metabolites and calcium ions. The combination of Lattice Boltzmann modeling with data obtained from non-invasive MR imaging …
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Protein degradation in rat skeletal muscle : intracellular, non-lysosomal enzyme systems and their endocrine control
… International, In Press). Membrane-free washed myofibrils derived from rat skeletal muscle homogenates contained a chymostatin-sensitive protease(s) which acted on associated myofibrillar proteins, at an optimum pH of 8.5, much less rapidly at low ionic strength (insoluble myofilaments) than at …
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Characterisation of the reaction of 1,4-phenylenebismaleimide with Ca²⁺-ATPase and elucidation of the intramolecular crosslink site
… that removes calcium from the sarcoplasm and myofibrils to allow muscle relaxation. The sulfhydryl crosslinker, 1,4- phenylenebismaleimide, reacts with Ca²⁺-ATPase (110 kD) to form a species with an apparent molecular weight of 125 kD, as well as dimers and high order oligomers, on SDS-P AGE. …
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An artificial skeletal muscle for use in pediatric rehabilitation robotics
… The artificial muscle consisting a bundle of 3 myofibrils, each which containing five actuators in series, generated 22º of elbow flexion in the sagittal plane with 224 º/s angular velocity, under 1 N of tensile load. ☐ These results demonstrated CT-SDEAs’ potentials and limitations in …
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Modelling Brugada Syndrome using induced pluripotent stem cells
… cardiomyocytes showed organisation of myofibrils, ultrastructure and calcium handling more similar to foetal than adult cardiomyocytes. Brugada Syndrome patient-specific cardiomyocytes were structurally indistinguishable from healthy control iPS cell line-derived cardiomyocytes. …
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Postmortem energy metabolisms' role in calpain activation, inhibition, and subsequent function
… at 0, 1, 2, 7, and 14 d postmortem. Purified myofibrils served as substrates to monitor degradation of desmin and troponin-T, two surrogates of muscle proteolysis postmortem. Minimal proteolysis occurred with early postmortem samples (0 and 1 d), while proteolytic activity increased notably by …
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Discovery of New Regulatory Proteins and Mechanisms in Muscle Biology and Disease
In an effort to discover new regulators of muscle function, we identified a novel muscle-specific protein, Klhl40. Genetic deletion of Klhl40 in mice results in a nemaline myopathy-like phenotype with disruption of sarcomere function causing neonatal lethality. Nemaline myopathy (NM) typically …