Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 113 for “"myoblasts"”.
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Hexose Transport In L6 Rat Myoblasts
A number of different approaches were used in characterizing the nature and the regulation of the hexose transport system in the permanent cell line, L6. This rat myoblast line can differentiate in culture to form multinucleated myotubes, but in this study, only the undifferentiated cells were …
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Burn Serum Stimulates Mitochondrial Fission in C2C12 Myoblasts
… fusion in response to a burn serum challenge in myoblasts in vitro. METHODS: Cultured murine myoblasts, C2C12 cells, w ere exposed to 10% rat serum isolated either from 40% total body surface area (TBSA) scald burn rats or control rats. Cells were then labeled with MitoTracker Green dye and live …
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Impact of Primary Myoblasts on Macrophage Polarization In-Vitro
… An alternative cell-based therapy candidate are myoblasts, or muscle progenitor cells. Myoblasts increase arteriogenesis in murine models and are known to interact with macrophages, which are immune cells that are primary regulators of arteriogenesis. Macrophages can polarize to inflammatory (M1) …
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Effects of three-dimensional culture conditions on skeletal muscle myoblasts
… Culture Conditions on Skeletal Muscle Myoblasts\r\n\r\nPublication No._____________\r\n\r\n\r\nMichele Lynn Marquette, Ph.D.\r\nThe University of Texas Medical Branch, 2007\r\n\r\nSupervisor: Marguerite A. Sognier\r\n\r\nThe objectives of this research were to: 1) develop a …
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Identification And Characterization Of The Hexose Transporters In Rat L6 Myoblasts
… systems are present in undifferentiated rat L6 myoblasts. These hexose transport systems were identified and characterized in this study using a number of different approaches. Cytochalasin B (CB), a potent inhibitor of hexose transport, was first used as a probe for these systems. CB binding …
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Roles of cholesterol in the proliferation and differentiation of bovine myoblasts
… the proliferation and differentiation of bovine myoblasts. In the first experiment, myoblasts isolated from Angus or Angus crossbred steers were cultured with 2% lipoprotein deficient fetal calf serum (LPDS) or normal fetal calf serum. Culturing with LPDS did not alter the cytosolic or membrane …
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Behaviour of human myoblasts in vitro: role of ageing and inflammatory cytokines
… stem cells known as satellite cells in situ and myoblasts ex vivo. The work in this thesis has used a cell culture approach to study the behaviour of human myoblasts and compare the inherent effects of age (by studying myoblasts taken from young and old people) and using an in vitro model of …
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The Immediate Early Response of Proliferating Myoblasts to One Bout of Electrical Stimulation
… of electrical stimulation on proliferating C2C12 myoblasts, in order to determine what induces the long term reductions in cell number associated with electrical stimulation. The results indicate that stimulation alters intracellular processes within these cells, promoting cell cycle arrest and …
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Uptake of Small EVs by Skeletal Muscle Myoblasts In Vitro and Subsequent Myogenic Response to Cargo
… EVs were generated from proliferating C2C12 myoblasts cultured in EV-depleted media and isolated using differential ultracentrifugation. Nanoparticle tracking analysis confirmed that the majority of isolated particles were within the expected size range (mean diameters below 200 nm). Cell …
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Integrin Alpha Subunit Ratios, Cytoplasmic Domains, and Growth Factor Synergy Regulate Muscle Proliferation, Differentiation, and Signaling
… phenotypes. The $\alpha$5 transfected myoblasts remain in the proliferative phase and are differentiation inhibited. Myoblasts overexpressing the $\alpha$6 subunit exhibit inhibited proliferation and substantial differentiation. Antisense suppression of endogenous quail $\alpha$6 …
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Roles of Growth Hormone, Insulin-Like Growth Factor I, and Sh3 and Cysteine Rich Domain 3 in Skeletal Muscle Growth
… from cattle and allowed to proliferate as myoblasts or induced to fuse into myotubes in culture. GH increased protein synthesis without affecting protein degradation in myotubes; GH had no effect on proliferation of myoblasts; GH had no effect on IGF-I mRNA expression in either myoblasts or …
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Impact of Leucine and HMB on Acute Muscle Damage: Focus on Repair and Regeneration in vivo and in vitro
… of age on the migration and fusion potential of myoblasts in the absence or presence of leucine or HMB supplementation and finally; 3) dynamic proteome profiling techniques using control and replicatively aged myoblasts and myotubes as models. Results: Post eccentric exercise, at 48 h there was a …
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Mechanisms of Ionophore Toxicoses: Laidlomycin Propionate Toxicosis in the Horse and Monensin, Laidlomycin, and Laidlomycin Propionate in Muscle Cultures
… were evaluated after 24-hour exposure in 3-day (myoblasts) and 14-day ($>$90% myotubes) cultures of E-63 rat skeletal muscle cells. Each of these ionophores induced dose-dependent increases in lactate concentrations, decreases in pyruvate concentrations, and decreases in accumulations of NR. …
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TBX2 IS INVOLVED IN MYOGENESIS AND ITS DEREGULATION PROMOTES TUMORIGENESIS IN RHABDOMYOSARCOMA
… found that TBX2 was expressed in both embryonic myoblasts and adult proliferative satellite cells, but was quickly down regulated during muscle differentiation in mouse models, which suggests an important function of TBX2 in the early myogenesis. Using molecular and cellular biology approaches we …
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Epigenetic Regulation of Muscle Stem and Progenitor Cells
… and progenitor cells. Loss of Mll1 in cultured myoblasts reveals an essential role for expression of the myogenic specification gene Pax7. Mll1 KO results in a minor decrease in Pax7 mRNA and a strong decrease of Pax7 protein. While MLL1 was found to bind the Pax7 promoter, Mll1 KO results in a …
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The myocyte enhancer factor-2 (MEF2) family mediates complex gene regulation in skeletal and cardiac myocytes
… Acute ablation of Mef2 expression in skeletal myoblasts revealed a required role for MEF2A activity in myoblast differentiation that was not shared with the other MEF2 factors. We hypothesized that a transcriptomic level analysis of Mef2-deficient skeletal myoblasts would reveal distinct …
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H36-alpha7 is a novel integrin alpha chain that is developmentally regulated during skeletal myogenesis
… H36-$\alpha$7 mRNA is present in replicating myoblasts: expression increases upon terminal differentiation and is markedly reduced in developmentally defective myoblasts. In addition, H36-$\alpha$7 mRNA is not detected in C3H10T1/2 cells. It is in myotubes derived from myoblasts obtained by …
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Role of MicroRNAs in Human Skeletal Muscle Tissue Engineering In Vitro
… and miR-696 in isolated primary human skeletal myoblasts would lead to accelerated differentiation of tissue-engineered muscle constructs with higher proportion of Type I myofibers and that are capable of significantly increased active contractile forces when subjected to electrical stimulus. …
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Developmental regulation of the alpha(7) integrin chain during myogenesis
… $\alpha$7B form is expressed both in replicating myoblasts and in differentiated myotubes and adult fibers. The $\alpha$7A and $\alpha$7C forms are only detected after myoblasts have undergone terminal differentiation. These three isoforms contain both a common as well as distinct potential …
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Identifying the signalling pathway of a novel Myostatin Splice Variant (MSV)
… opposes Mstn and stimulates the proliferation of myoblasts provided the impetus for the investigations in this thesis. The splicing of MSV was restricted to the Cetartiodactyl clade of mammals, and MSV may represent an intragenic regulator of Mstn. Thus, the studies undertaken in this thesis were …
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