Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"myoblast differentiation"”.
-
Roles of proteasome, arachidonic acid, and oxytocin in bovine myoblast proliferation and differentiation
… factors and mechanisms that control bovine myoblast proliferation, differentiation, and fusion. Three studies were conducted during this project. The objective of the first study was to determine the effect of oxytocin (OXT) on myoblast proliferation, differentiation and fusion. Treating …
-
Role of JAK3 in Myogenic Differentiation
Skeletal muscle differentiation is regulated by transcription factors, including members of the myogenic regulatory factors (MRFs) family and many signaling pathways. The JAK1 and JAK2 pathways are known to each have different effects on myoblast proliferation and differentiation; however, the role …
-
Genome-wide identification of enhancers, transcription factors, and mechanisms that control skeletal muscle differentiation in cattle
… potentially novel mechanisms that mediate myoblast differentiation, a process whereby the mononuclear muscle precursor cells myoblasts express skeletal muscle-specific genes and fuse with each other to form multinucleated myotubes. Comparing gene expression profiles in C2C12 cells, a widely …
-
Molecular mechanisms of mammalian cell survival and differentiation
… underlying mammalian cell survival and differentiation. In particular, I investigated the regulation of mammalian target of rapamycin (mTOR), an evolutionarily conserved Ser/Thr kinase that integrates signals from nutrient availability, growth factors, differentiation inducers, and …
-
Optimizing Ex Vivo Culture of Satellite Cells
… governing satellite cell proliferation and differentiation. We must also have tools that allow us to control these mechanisms. In this thesis, I tried to understand in vitro behavior of satellite cells and to find factors that can control their differentiation pathways. I showed the effect …
-
Roles of cholesterol in the proliferation and differentiation of bovine myoblasts
… membrane cholesterol in the proliferation and differentiation of bovine myoblasts. In the first experiment, myoblasts isolated from Angus or Angus crossbred steers were cultured with 2% lipoprotein deficient fetal calf serum (LPDS) or normal fetal calf serum. Culturing with LPDS did not alter …
-
Human FRG1 is an Actin-Bundling and a RNA-Associated Protein with Distinct Subcellular Localizations
… cytoplamic, and sarcomeric protein. During myoblast differentiation, FRG1’s subcellular distribution changed dramatically with FRG1 eventually associating with the matured Z-discs. The Z-disc localization is confirmed in mouse myofibers, suggesting FRG1 may have a muscle-specific function …
-
Ruolo della fosfolipasi C-β1 nel differenziamento miogenico: identificazione di nuovi target nucleari
… and cyclin D3 is highly induced during skeletal myoblast differentiation. We have previously shown that PLC-β1 activates cyclin D3 promoter during the differentiation of myoblasts to myotubes, indicating that PLC-β1 is a crucial regulator of mouse cyclin D3 gene. Here we report that PLC-β1 …
-
Mechanisms of mTOR Signal Transduction Regulating Cell Growth and Differentiation
… miR-1, which is up-regulated during differentiation. mTOR is essential for miR-1 expression in C2C12 cells and in vivo in regenerating myofibers. It regulates the transcription of miR-1 through its upstream enhancers, and this regulation is at least partially mediated by the myogenic …
-
Regulation of skeletal myogenesis by the mTOR signaling network
… to myogenic lineage and differentiate into myoblasts, which then fuse to form multinucleated myofibers. Post-natal growth, repair, and maintenance of skeletal muscle are dependent on muscle satellite cells, which in response to stimuli differentiate and fuse to form new myofibers. …
-
Predicting molecular markers of development and regeneration through integrative analysis of high-throughput genomics data
… exhibiting potential control over Myf5 during myoblast differentiation.
-
Non-canonical functions of leucyl-tRNA synthetase: Mechanism of cell growth and skeletal myogenesis
… master regulator of cell growth, proliferation, differentiation and metabolism, and it has emerged as an attractive target of intervention in several human diseases. mTORC1 assembles a signaling network that senses and transduces a variety of environmental and cellular cues, including amino acid …
-
The myocyte enhancer factor-2 (MEF2) family mediates complex gene regulation in skeletal and cardiac myocytes
Regulation of striated muscle differentiation and development are complex processes coordinated by an array of transcription factors. MEF2 is a crucial transcription factor required for muscle differentiation, but the roles of the individual MEF2 family members, MEF2A-D, have not been extensively …
-
Integrin Alpha Subunit Ratios, Cytoplasmic Domains, and Growth Factor Synergy Regulate Muscle Proliferation, Differentiation, and Signaling
Integrin function in muscle differentiation was addressed by ectopic expression of integrin alpha subunits in primary quail skeletal muscle. Ectopic expression of either the human or $\alpha$5 or the chicken $\alpha$6 subunit produced contrasting phenotypes. The $\alpha$5 transfected myoblasts …
-
Investigating the Impact of Biochemical and Mechanical Stimuli on Motor Neuron Growth
… when mechanical stimulation was applied after myoblast differentiation. In future research, we are exploring the biochemical and magnetic stimulation on our neuromuscular co-culture and actuate the myoblasts at an earlier cellular stage to impact alignment. In conclusion, our studies found that …
-
Effects of three-dimensional culture conditions on skeletal muscle myoblasts
… Culture Conditions on Skeletal Muscle Myoblasts\r\n\r\nPublication No._____________\r\n\r\n\r\nMichele Lynn Marquette, Ph.D.\r\nThe University of Texas Medical Branch, 2007\r\n\r\nSupervisor: Marguerite A. Sognier\r\n\r\nThe objectives of this research were to: 1) develop a …
-
Roles of Growth Hormone, Insulin-Like Growth Factor I, and Sh3 and Cysteine Rich Domain 3 in Skeletal Muscle Growth
… from cattle and allowed to proliferate as myoblasts or induced to fuse into myotubes in culture. GH increased protein synthesis without affecting protein degradation in myotubes; GH had no effect on proliferation of myoblasts; GH had no effect on IGF-I mRNA expression in either myoblasts or …
-
The Change in the Concentration Levels of Extracellular Vesicles and of Cytokines in the circulatory blood system in Response to different exercise protocols
… concentration levels. As a measure of changes in differentiation of muscle cells the expression level of Myogenin in EV-treated C2C12 cells by plasma samples was determined by qPCR. The experimental conditions and cell model for the differentiation of C2C12 was standardised and the expression of …
-
Muscle-derived cytokines: novel regulators of skeletal myogenesis
… cells proliferate to produce many daughter myoblasts at the injury site. These daughter myoblasts subsequently undergo an ordered series of steps to remake functional syncytial myofibers, including migration, cell cycle withdrawal, expression of pro-differentiation genetic programs, and …