Global ETD Search
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Showing 1 to 5 of 5 for “"myo-inositol oxygenase"”.
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Towards biosensor-assisted directed evolution of myo-inositol oxygenase
… for this work was the directed evolution of myo-inositol oxygenase (MIOX), the enzyme that catalyzed the penultimate, rate-limiting step in the metabolic production of glucarate from glucose in E. coli. Here, we develop a biosensor that recognizes glucuronate, the direct product of MIOX, and …
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Biosensor-based strategies for improving pathway production in Escherichia coli
… to be limited by the two downstream enzymes myo-inositol-1-phosphate synthase (MIPS) and myo-inositol oxygenase (MIOX). This work develops and deploys a biosensor that recognizes a pathway intermediate in order to overcome both limitations. A biosensor for myo-inositol (MI) was developed …
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Retrobiosynthesis of D-glucaric acid in a metabolically engineered strain of Escherichia coli
… is the second recombinant step, catalyzed by myo-inositol oxygenase (MIOX), whose activity is strongly influenced by the concentration of the myo-inositol substrate. To increase the effective concentration of myo-inositol, synthetic scaffold devices were built from protein-protein interaction …
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Molecular Characterization of Two myo-Inositol Oxygenases in Arabidopsis thaliana
… stresses. One stress-responsive system involves myo-inositol (Ins). Ins is a precursor for cell wall components, inositol trisphosphate (Ins(1,4,5)P3) and phosphatidylinositol phosphate signaling molecules, and an alternate ascorbic acid (AsA) synthesis pathway. The enzyme, myo-inositol oxygenase …
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Untersuchungen zur Biosynthese der pflanzlichen Zellwand = [Identification and characterization of genes involved in plant cell wall synthesis]
… UDP-glucuronic acid epimerase (GAE) and the myo-inositol oxygenase (MIOX) families. For the three-membered RHM family relative ubiquitous expression was shown using variuos methods. For one of these genes, RHM2, T-DNA lines could be obtained. Moreover, the transcription of the whole family …