Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"mycolic acid"”.
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Genetic engineering of recombinant anti-mycolic acid antibody fragments for use in tuberculosis diagnostics
Mycolic acids are long chain lipids from the cell walls of Mycobacterium tuberculosis. The Nkuku phage display library was previously used to obtain monoclonal antibody binders to mycolic acids. In total 11 binders were obtained of which one was selected (MAC10) for further investigation by genetic …
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Towards paper-based micro bio-sensing of biomarker anti-mycolic acid antibodies for TB diagnosis
… in the diagnosis of TB, the detection of anti-mycolic acid (MA) antibodies in patient sera has been shown to allow accurate diagnosis in HIV-positive, previously infected and TB exposed patients. MA is a unique lipid antigen of mycobacteria with anti-MA antibodies being formed early upon …
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Probing the Mycobacterial Cell Envelope: CRISPR interference-mediated investigation of two essential genes in the galactan biosynthetic pathway
… probe that selectively incorporates into the mycolic acid layer of the cell envelope. Morphological analyses revealed altered DMN-Tre signal along the medial axis of the cell, indicating a potential disruption of mycolic acid biosynthesis when galactan biosynthesis is compromised. Notably, …
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Electrochemical impedance spectroscopy and surface plasmon resonance for diagnostic antibody detection
… this in the case of detection of biomarker anti-mycolic acid antibodies for TB diagnosis has been provided in the Mycolic acid Antibody Real-Time Inhibition assay (MARTI) by our research group. Although already patented and published, MARTI is not yet a feasible diagnostic test due to slow sample …
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Channel-forming proteins in the cell wall of amino acid-producing Corynebacteria
… C. efficiens a member of the diverse group of mycolic-acid containing actinomycetes, the mycolata. These bacteria are potent producer of glutamate, lysine and other amino acids on industrial scale. The cell walls of most actinomycetes contain besides an arabinogalactan-peptidoglycan complex …
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Automated synthesis and evaluation of potential new anti-microbial agents
… support for the synthesis of some fatty acid derivatives, containing an isoxazoline group, which may inhibit mycolic acid synthesis in mycobacteria. Both the PEG-bound products and the cleaved, isolated products themselves were tested against M.fortuitum and some low levels of …
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Effect of Lactoferrin to Increase Drug Permeability of Primary Pulmonary Mycobacterial Granulomas
… dissemination. The major mycobacterial surface mycolic acid, trehalose 6,6'-dimycolate (TDM), functions in multiple ways to enhance immune cell recruitment of sites of infection, to induce inflammation and granulomatous responses, and to initiate survival strategies for the organism inside …
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Filamentous Foaming in Activated Sludge Systems: A Study Combining Molecular and Engineering Approaches
… been associated with the presence of filamentous mycolic acid-containing actinomycetes (mycolata) and ""Microthrix parvicella "". However, the relationship of filament populations to foaming incidents is not fully understood. This is in part due to the limitations of morphology- and culture-based …
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Targeting Innate Immune Memory to Alleviate Monocyte Exhaustion and Chronic Inflammation
… that co-treatment of monocytes with methoxy-mycolic acid (M-MA) effectively impedes the phenotype of exhausted monocytes induced by repeated exposure to LPS, including blocking the expansion of exhausted Ly6Chi/CD38hi/PD-L1hi monocytes and restoring the expression of CD86. Functionally, M-MA …
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Design and synthesis of inhibitors of dTDP-D-glucose 4,6-dehydrate (Rm1B), and enzyme required for dTDP-L-rhamnose production in M. tuberculosis
… and arabinogalactan portions of the mycolic acid-arabinogalactan-peptidoglycan complex, a significant part of the mycobacterial cell wall. The mounting level of biochemical understanding of this pathway and its importance in bacterial cell wall biosynthesis indicates that it is not …
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Design, Synthesis, and Evaluation of Novel Antimycobacterial Agents With Enhanced Pharmacokinetic Profiles
… inhibition of bacterial cell wall biosynthesis. Mycolic acids, which are α-alkylated β-hydroxylated fatty acids, are the primary lipid constituents of the mycobacterial cell wall and are synthesized intracellularly in the cytoplasm. Mycobacterial membrane protein large 3 (MmpL3) is an essential …
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Investigating the effect of a Mycobacterium tuberculosis inhA promoter mutation in cell envelope composition and morphology
… and second-line drugs respectively that target mycolic acid (MA) synthesis. MA are characteristic components of mycobacterial species’ cell wall and contribute to the impermeability of cell wall by virtue of its hydrophobicity. IPMs result in a transcriptional upregulation of inhA, and mabA, …