Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 157 for “"mycobacteria"”.
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Efflux-Mediated Multidrug Tolerance in Replicating Mycobacteria
… is historically attributed to the infecting mycobacteria becoming dormant within the host so that they are no longer killed by currently used antitubercular drugs that target actively growing bacteria. The dormancy model of tolerance has been the center of antitubercular drug research with …
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Regulation of Metabolism in Mycobacteria and Cyanobacteria
Bacteria encounter changes in their environment and must adapt to these changes in order to survive. Their ability to adapt is determined by their capacity to efficiently regulate their cellular processes. The mechanisms of bacterial regulation at the transcriptional level have been investigated by …
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Determinants of innate immune responses to mycobacteria
… dendritic cells and granulocytes recognise mycobacteria and initiate immune responses such as phagocytosis, cytokine production and expression of maturation markers. The type and magnitude of innate responses to mycobacteria may determine the subsequent adaptive responses generated. Our aims …
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Replication fidelity in the mircroevolution of mycobacteria
… fidelities of the dnaE1- and dnaE2-encoded mycobacterial PolIIIα subunits under conditions of genotoxic stress. To this end, the role in DnaE1 intrinsic fidelity of highly conserved PHP domain residues was explored by site-directed replacement of targeted amino acids, resulting in a panel …
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Early-life immunity and susceptibility to Mycobacteria
The naïve and not-yet developed infant immune system exhibits heightened susceptibility to external factors (e.g pathogens), and is shaped by these and others, such as maternal immunity. However, we do not yet fully understand their impact on development of infant immunity. A better understanding …
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Comprehensive definition of Ser/Thr/Tyr phosphorylation in mycobacteria: towards understanding reprogramming of normal macrophage function by pathogenic mycobacteria
… Understanding the behaviour of pathogenic mycobacteria in the macrophage is also important to the discovery of new drug targets. In this thesis, we employed state of the art mass spectrometry techniques, which allowed us to unpack the biology of this bacterium in different growth …
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Elucidation of mechanisms of antibiotic subversion in mycobacteria
… most significantly the low permeability of the mycobacterial cell wall, the operation of various drug inactivating systems, and the activity of efflux pumps. This study aimed to investigate the role of various components of the "intrinsic resistome" that limit the efficacy of antitubercular …
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Methods for the Study of Galactofuranose in Mycobacteria
… in situ for facile preparation of the mycobacterial galactan utilizing the sugar mutase UDP-galactopyranose mutase. We used this method to generate up to 10 milligrams of polymer and demonstrated that it could be selectively functionalized. Second, we leveraged the rapidly expanding set …
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Cigarette smoke and human pulmonary immune responses to mycobacteria
… of key cytokines implicated in defences against mycobacteria were observed, not attributable to impairment of mycobacterial uptake by cigarette smoke extract exposure. Furthermore, control of intracellular mycobacterial growth was impaired by cigarette smoke extract exposure.
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Inhibition of enzymes from mycobacteria using fragment-based approaches
… of fragment-based approaches for two essential mycobacterial target proteins, fumarate hydratase (fumarase) from Mycobacterium tuberculosis (Mtb) and tRNA (m1G37) methyltransferase (TrmD) from Mycobacterium abscessus (Mab). With Mtb fumarase a high-throughput screening (HTS) hit was used to …
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Identification and isolation of growth-phase specific proteins of mycobacteria
The aim of this project was to identify growth phase-specific heat shock proteins of Mycobacterium smegmatis LR222. A growth curve was constructed using the ATP assay. This method was shown by B. A. Ntolosi to be the most accurate indicator of when the organism entered the various phases of growth. …
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Subcellular localization and visualization of RecA and ImuAʹ in mycobacteria
… immune and drug assault. The potential target: mycobacterial DNA metabolism. For this, an in-depth understanding of the mechanisms of DNA repair and mutagenesis in mycobacteria is required. RecA and ImuAʹ are DNA damageinducible proteins implicated in DNA damage repair and tolerance in Mtb. RecA …
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Non-tuberculous Mycobacteria in The Gambia: Prevalent Species, Carriage and Disease
… disease caused by non-tuberculous mycobacteria (NTM) has gained attention, in part, because of its increased recognition especially in the elderly and immunocompetent population. This is an emerging problem of public health significance. With the increasing incidence of case reports …
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CRISPRI-based high-throughput functional genomic approaches for use in mycobacteria
… sequencing, have been developed and applied to mycobacteria in a variety of conditions; however, they have several limitations, particularly in their ability to study genes essential for viability. The recent optimisation of inducible CRISPR-interference for mycobacteria offers the potential to …
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Residential Exposure to Non-tuberculous Mycobacteria: Sources and Associations with Pulmonary Disease
The human health impacts of non-tuberculous mycobacteria (NTM) in home environments remain poorly understood. To better understand the association between NTM colonization of POU sources in homes and MAC pulmonary disease (MAC PD), we continued a case-control study of Washington and Oregon …
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Chitosan and PLGA microspheres as drug delivery system against pulmonary mycobacteria infections
Various types of rifampicin (RIF)-loaded microparticles were compared for their stability during nebulization. Poly(lactide-co-glycolide) (PLGA), chitosan (CHT) and PLGA/CHT microparticles (MPs) were prepared by emulsion, precipitation or spray-drying techniques. MPs ability to be nebulized (NE%) …
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A Tet-repressible regulation system in mycobacteria for application to vaccine design
Includes abstract. Includes bibliographical references (leaves 116-123).
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Isolation and characterization of plasmids from human and environmental isolates of mycobacteria
Human clinical (n=131) and environmental (n=226) isolates of the Mycobacterium avium-intracellulare and M. scrofulaceum (MAIS) complex were screened for plasmids in an effort to increase knowledge about the genetics and epidemiology of these pathogenic bacteria. Approximately 50% of the clinical …
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Construction and Use of a Transposon for Identification of Essential Genes in Mycobacteria
… origin for more efficient mutagenesis of mycobacteria. In a preliminary genetic screen using the model organism Mycobacterium smegmatis, 13 mutants with ATc-dependent growth were identified. Identification of the insertion sites by cloning and sequencing indicated that there were nine …
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