Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"mutator"”.
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Evolutionary Instability Of Genomic Mutation Rate In Rapidly Adapting Asexual Mutator Escherichia Coli Populations
Alleles conferring higher mutation rates (mutators) can fix in asexual populations through a process called ‘mutator hitchhiking’. Theory predicts that repeated mutator hitchhiking can occur in an adapting asexual mutator population. I tested this prediction in two settings: a mutL- population …
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Taming a Potent Mutator: Identification and Characterization of Novel Mechanisms of Regulating Antibody Diversification in B-Lymphocytes
<p>Often the needs of an organism exceed the number of genes in the genome. Thus, modification of the genes, themselves, or of the gene products is necessary. This becomes particularly important in cells of the immune system, which have to combat a virtually infinite array of foreign pathogens. …
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Análise comparativa de transposons classe II (CACTA e Mutator) nos genomas de Vigna unguiculada, Phaseolus vulgaris e Medicago truncatula
… em V. unguiculata. Todos apresentaram CACTA e Mutator entre as três superfamílias de transposons mais abundantes no genoma. Além disso, a identificação de transcritos em cultivares contrastantes quanto à tolerância à seca em V. unguiculata mostrou que a cultivar tolerante apresentou maior …
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Dynamic Mutation Rates and Extinction in Sexual Organisms
… infinite randomly-mating populations. Germ-line mutator alleles, alleles that increase the genomic mutation rate, frequently occur when mutations alter the structure or regulation of genes involved in DNA repair, a fraction of the genome called the "repair genome”. The repair genome is a subset …
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Isolation of antimutator yeast strains: an exploration of DNA replication fidelity
… that lack Pol proofreading or MMR exhibit a mutator phenotype, manifested as a 10- to 100-fold increase in spontaneous mutation rate. Haploid yeast strains with combined defects in Pol proofreading and MMR initially divide, but succumb to error-induced extinction and fail to form viable …
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Evolution of Pseudomonas aeruginosa in the Cystic Fibrosis Lung
… also be increased by the activity of so-called mutator strains that have high background mutation rates. We sought to discover large- and fine-scale patterns of genomic changes in 49 strains representing 16 longitudinal series that ranged up to 25 months in duration. A subset of these contained …
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Evolution of Pseudomonas aeruginosa in the Cystic Fibrosis Lung
… also be increased by the activity of so-called mutator strains that have high background mutation rates. We sought to discover large- and fine-scale patterns of genomic changes in 49 strains representing 16 longitudinal series that ranged up to 25 months in duration. A subset of these contained …
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Role of SMU Homologues in Pre-mRNA Splicing During Maize and Arabidopsis Development
… maize (Zea mays ssp. mays) opaque mutant, mto38 (Mutator-taggedopaque 38), was shown to cosegregate with a Mutator-tagged genomicfragment. Sequence analysis of the DNA indicated that it contained a genesimilar to the smu-2 (suppressor of mec-8 unc-52) gene in nematodes. Previous studies showed …
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Hematopoiesis: Functional Insights by Cell Fate Conversion
… several premature aging phenotypes. These “mutator mice” displayed several hematopoietic abnormalities associated with aging, including anemia and defects in lymphopoiesis. However, several hallmarks associated with HSC aging was lacking. In addition, the observed phenotypes appeared to …
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An experimental investigation into the mechanisms of bacterial evolution
… qPCR assay of competition between wild-type and mutator phenotype <em>E. coli</em>. Mutator phenotype has an elevated mutation rate due to defects in DNA proofreading and repairing system. By introducing antibiotic selective pressure, I will examine the fixation probability of mutators competing …
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Modeling and analysis of market share dynamics in a duopoly subject to affine feedback advertising policies and delays
… of market share dynamics based on the replicator-mutator model from evolutionary game theory. This is done by identifying strategies with the entries of a preference matrix consisting of the choice preferences of firms by clients and the mutation matrix representing transition probabilities from …
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Identification of proteins interacting with the human mismatch repair protein MLH1
… Rat-1 cMYCERTM system was shown to result in a mutator phenotype in these cells. The results suggest there may be a link between the mutator phenotype, induced through overexpression of c-MYC, and loss of MMR. Overexpression of c-MYC, which is associated with many cancers, may result in the …
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Tumour evolution in ovarian cancer using high-throughput genomics technologies
… (median size of tandem duplications was 350kb). Mutator phenotypes in both cases arose early in progression and persisted, but the tumour with HR deficiency showed evidence of re-stabilising its ,"genome and lost platinum sensitivity after a revertant BRCA2 mutation restored its HR function. A …
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Engineered tools for studying the malaria parasite plasmodium falciparum
… to improve and demonstrate usability. Third, a mutator strain will be engineered and defined for eventual use in the study of drug resistance and the characterization of the resistance potential of anti-malarial drugs.
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Telomeric Regulation of Chromosomal Instability In Colorectal Cancer
… malignant adenocarcinoma. While CIN provides a mutator mechanism for cancer-relevant genomic events, its role in shaping tumor biology during carcinogenesis is not well understood. Here, we explored the molecular and biological impact of telomere dysfunction and associated CIN <em>in vivo</em> …
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New approaches to antimalarial target deconvolution and measuring fitness effects of Plasmodium falciparum mutations implicated in drug resistance.
… in the selection. The use of so called hyper-mutator lines that have lost the ability to proofread during transcription has been established as a way to increase mutation rate and therefore the rate at which resistance is able to develop in yeast and bacteria. Recently our lab generated a …
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Endogenous RNAi pathways are required in neurons for dauer formation in Caenorhabditis elegans
… experiments demonstrate that disruption of the Mutator proteins or the nuclear argonaute CSR-1 result in differential dauer-deficient phenotypes that are dependent upon the environmental stress. We found that these RNAi components function in individual neurons upstream of TGF-β and insulin …
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