Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"mutant screen"”.
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Plant responses to environmental stimuli: A screen for mutants aberrant in TCH4 expression behavior, and a full-genome assessment of touch and darkness inducibility
… occur. I describe herein a proof-of-concept mutant screen for identifying mutants aberrant in their responses to a variety of stimuli including heat, cold, touch, and darkness. Such mutants will undoubtedly serve as tools to investigate the molecular pathways by which plants detect and …
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The regulation of genes involved in trichome development
… SIAMESE (SIM) was first identified in a mutant screen in the Larkin lab. This dissertation demonstrates that this gene encodes a novel type of cell-cycle regulator with several homologs in Arabidopsis and other plant species. SIM and one of its homologs in Arabidopsis were shown to be …
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Identification of an extracellular adenosine 5'-triphosphate receptor in Arabidopsis thaliana
… In this dissertation study, we describe a mutant screen that identified a key molecular component involved in extracellular ATP recognition in Arabidopsis thaliana. The gene identified by isolation of an ATP-insensitive mutant was termed dorn1 (Does not Respond to Nucleotides 1), encoding a …
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The genetic control of stem mechanical properties in Brassica Napus and Wheat
… genes were validated in an Arabidopsis thaliana mutant screen, which revealed GAUT5, SAUR72 and a pectin methylesterase (AT3G12880), as key contributors to stem mechanical strength. Xylan acetylation and pectin methylesterification are both known to impair saccharification efficiency of …
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The Establishment and Regulation of Melanocyte Stem Cells in Zebrafish
… of kit heterozygotes to wild type. The mutant heterozygotes have normal MSC recruitment and normal proliferation, differentiation, and survival of daughter cells. The mutant has defective MSC establishment, with this defect being quantitatively sufficient to explain the regeneration …
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Developmental Functions Of Mir156 And Mir157 In Arabidopsis
… also inspired us to perform a sensitized mutant screen using miR157 mutants as a genetic background. This screen identified the B3 domain transcription factor VALs as regulators of miR156 during vegetative development. val mutations led to increased miR156 abundance as a result of elevated …
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Protein Phosphatase 2A Function in Response to Salt Stress in Arabidopsis thaliana: Defining Pathways and Genetic Interaction Networks
… phenotypes. A subset of the A and C subunit mutants exhibited root skewing or curling phenotypes when grown on vertically-oriented plates supplemented NaCl. I characterized the root response of a1rcn1 and c4 mutants and found that the root phenotype was sodium specific and influenced by NaCl …
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Identification and characterization of factors involved in DNA demethylation and anti-silencing in Arabidopsis
… demethylation pathway, we developed two genetic screening systems in <em>Arabidopsis </em>to identify new components involved in the DNA demethylation. In the first system, which is based on 35S promoter driven <em>SUC2</em> (sucrose transporter 2) transgene, a mutant (<em>91-1</em>) was isolated …
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Characterisation of mutants involved in Phytochrome A nuclear import and signal transduction
… an Ethyl Methane Sulphonate (EMS) mutagenesis screen was performed using a transgenic line expressing phyA: GFP fusion protein. Aim of the screen was to isolate and characterise Phytochrome A (phyA) nuclear transport mutants. The screening was performed in two steps. First, the mutagenised …
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On the molecular pathways supporting the development of structurally coloured cuticle in Hibiscus trionum
… cell wall for the development of striations. Mutant screen experiments identified 2 mutant lines characterised by the presence of smooth patches of cells and the presence of conical cells in one of them. Further characterisation of the mutant phenotypes suggested the importance of the cell …
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Characterization of memories and ignorant (S6KII) mutants in operant conditioning in the heat-box
… learning. Thus, amnesiac, rutabaga, and dunce mutants have an impaired learning / memory. Searching for, yet unknown, genes and signaling cascades involved in operant conditioning, a Drosophila melanogaster mutant screen with 1221 viable X-chromosome P-element lines was performed. 29 lines with …
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Identification and characterisation of novel zebrafish brain development mutants obtained by large-scale forward mutagenesis screening
… situation is the creation of specific mutants in single genes, and the zebrafish (Danio rerio) has proven to be an excellent model organism for this purpose. In this thesis, I report on two forward genetic screens performed to find new mutants affecting brain development, in particular …