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Showing 1 to 20 of 22 for “"mutS"”.
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Production and characterization of MutS for use in error correction
… the synthesis of new hyper-thermophilic MutS proteins, characterizing these proteins using instruments such as the circular dichroism spectrophotometer and the Evotec MF20, as well as perfecting old error correction protocols while designing several new ones.
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Targeted Drug Discovery: Targeting a Specific Conformation of MutS/MSH Proteins with Small Molecules
Mismatch repair proteins repair mismatches in the DNA and signal cell death in response to certain DNA lesions. Due to these distinct functions of MMR proteins, they are proposed to exist in at least two different conformations, "repair" and "death." The goal of this study was to exploit the …
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Development and road-testing of an inducible hypermutator strain of Pseudomonas aeruginosa
… mutation. In Pseudomonas aeruginosa (PA), the mutS gene is commonly mutated in hypermutator strains. MutS mutants display an elevated mutation rate (>100-fold, compared with the wild-type (WT)) and are often associated with the acquisition of antibiotic resistance in clinical PA isolates. In …
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Shall we dance? : investigating protein interactions implicated in Escherichia coli postreplicative mismatch repair using a bacterial two hybrid system
… multiple DNA repair systems to counteract these. MutS, MutL and MutH are integral elements of dam -directed repair (MMR), a mismatch recognition system involved in rectifying DNA replication errors. Moreover, MutS and MutL have been implicated in the effective endonucleolytic functioning of Vsr, …
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The role of mismatch repair in mediating cellular sensitivity to cisplatin : the Escherichia coli methyl-directed repair paradigm
… mutation in either of the mismatch repair genes mutS or mutL show near wildtype levels of resistance. A prevalent explanation for this observation is the abortive repair model, which proposes that in dam mutants, where the strand discrimination signal is lost, mismatch repair attempts futile …
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The role of mismatch repair and recombination in cellular responses to the DNA damaging anticancer drug Cisplatin
… cisplatin toxicity. The mismatch repair protein MutS recognized cisplatin-DNA adducts with 2-fold higher affinity than adducts of oxaliplatin, a cisplatin analog that does not elicit resistance in mismatch repair mutants. MutS recognized the major cisplatin DNA-adduct, the 1,2-d(GpG) intrastrand …
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Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris
… A genetically modified Pichia pastoris MutS strain, engineered to produce the insulin precursor, was used as the experimental system in this study in order to optimise the insulin production process. The experimental system developed in this study employed a two-stage fed-batch feeding …
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IN VITRO AND IN VIVO ACTIVITY OF DINB: ROLE IN MUCOID CONVERSION OF PSEUDOMONAS AERUGINOSA
… has lost mismatch repair function by deletion of mutS, the mucoid conversion frequency was drastically increased. Both with the single deletion dinB- as well as the double deletion mutS-dinB-, mucoid conversion is essentially abolished. This indicates a crucial role for both DinB and MutS in …
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DNA mismatch repair proteins in Tetrahymena thermophila
… from bacteria to humans. In Escherichia coli, MutS and MutL are the key proteins in MMR. In prokaryotes, the MMR proteins function as homodimers whereas in eukaryotes the MMR proteins come together as heterodimers. In the absence of MMR there is a great increase in mutation frequency and a …
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Deformability and Dynamics in Linear and Looped/Supercoiled DNA with Implications for Damage Sensing
… and initiates nucleotide excision repair; 2) MutS, which recognizes single mismatches or unpaired nucleotides introduced during replication and initiates mismatch repair. Using linear DNA with 3-bp mismatches as mock lesions for Rad4, we found that Rad4-specific substrates were more dynamic on …
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AN EXAMINATION OF BINDING DIFFERENCES AMONG MISMATCH REPAIR RECOGNITION PROTEINS WITH MISMATCH AND CISPLATIN DAMAGED DNA
… damage site. To examine these models, E. coli MutS, S. cerevisae MSH2-MSH6, and MSH2-MSH3 were expressed to perform fluorescence anisotropy binding experiments and to crystallize the proteins in complex with cisplatin damaged DNA for comparison to mismatch DNA binding. All three mismatch repair …
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The Role of Arabidopsis thaliana Mismatch Repair Proteins in Meiotic Recombination
… an analysis of the genome-wide distribution of MutS homolog 4 (MSH4). MSH4, and its binding partner MutS homolog 5, evolved from their ancestral role in MMR and now function exclusively to promote meiotic crossovers in the ZMM pathway. I present a genome-wide …
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Biochemical characterization of the E. coli Very Short Patch Repair pathway and its coordination with methyltransferase repair of 0⁶-methylguanine
… nick on the 5' side of the mismatched T. The MutS and MutL mismatch recognition proteins stimulate this activity, as cells lacking either of these proteins display diminished VSPR. Genetic studies also indicate that Pol I is responsible for removing and replacing a short tract of nucleotides …
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Studies of DNA repair and mutagenesis in Escherichia coli and Bacillus subtilis
… the studies described here have found that MutS and MutL, the key proteins of MMR, form discrete foci in response to mismatches. These MMR foci are the active sites of repair, and appear to assemble near the DNA polymerase at mismatched base-pairs. This is consistent with a model in which …
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Identifizierung und Charakterisierung eines Vsr-Homologen aus Bacillus stearothermophilus
… untersuchen zu können, wurden ein MutL- und ein MutS-Homologes aus Bacillus stearothermophilus aufgereinigt. Mit dem in dieser Arbeit verwendeten System konnte keine Stimulierung der Vsr.Bst-Aktivität durch MutL und/oder MutS beobachtet werden. Die Möglichkeit einer Steigerung der …
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Techniques for the identification and differentiation of Cronobactor species
… experiments and data analysis. First, the mutS-rpoS genomic region of Cronobacter spp. was examined. Though this region is highly variable in other related species, all seven Cronobacter spp. contained the same genes in this region, in the same order, indicating that these genes are not …
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Evolutionary Instability Of Genomic Mutation Rate In Rapidly Adapting Asexual Mutator Escherichia Coli Populations
… a mutL- population under lethal selection and a mutS- population under soft selection. In both experiments, the starting mutation rate was 100-fold higher than wild-type. In the lethal selection experiment, two replicate populations were exposed to a sequence of three different antibiotics. In …
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Soziomoralischer Kompetenzvergleich von Regel- und Waldorfschülern nach Grundlagen des Kohlberg-Modells
… von Regel- und Waldorfschülern mit Hilfe des „MUTs“ verglichen wird. In der anschließenden Schlussfolgerung werden die Forschungsergebnisse erläutert und zur Diskussion gestellt, wobei nach einer adäquaten Thesenbildung gesucht wird.
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A Test Planning System for Functional Validation of VHDL DSP Models
… the test benches including models under test (MUTs). This is a laborious and time-consuming process. Therefore, it is desirable to develop a high level approach to automating and planning these tasks. This dissertation presents a high level test planning system for functional validation of VHDL …
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Characterizing Chromosomal Aberrations in Cells Deficient for Both ATM and MSH2
<p>Ataxia telangiectasia mutated (ATM) and mutS homologue 2 (MSH2) are important DNA repair proteins that participate in DNA repair pathways to maintain genomic integrity. Mice deficient for ATM and MSH2 mice are viable. However, <em>ATM<sup>-/-</sup></em> mice show growth retardation, neurological …
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