Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"multivalent interactions"”.
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Characterizing Multivalent Interactions Between Folded Protein Domains and Intrinsically Disordered Regions or Peptide Substrates
<p>Protein-protein interactions (PPIs) play central roles in most biological processes. Studying PPIs is a fundamental step in understanding the molecular basis of cellular processes such as cell-cell contact, enzyme activity, and transient assembly of signaling complexes or cellular structures. In …
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MOLECULAR MECHANISMS OF THE FORMATION OF ENDOCYTIC PATCHES AND THE INITIATION OF ACTIN POLYMERIZATION IN FAST ENDOPHILIN MEDIATED ENDOCYTOSIS
… sites. This phase transition is triggered by multivalent interactions between EDP’s SH3 domain and the proline rich motifs found in its binding partners. The availability of the receptor TIL, which is a marker of receptor activation, promotes LPDEDP clustering on lipid membranes demonstrating …
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Compositional Control of Phase-Separated Cellular Bodies
… to be phase separated liquids organized by multivalent interactions among proteins and RNA molecules. Although many components of various cellular bodies are known, general principles that define body composition are lacking. We have proposed a model for the formation of cellular bodies that …
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Design, Synthesis and Study of Novel Multivalent Ligands - Toward New Markers of Cancer Cells
… towards cancer cells, we propose the use of multivalent interactions targeting specific receptor combinations at the cancer cell surface. In this thesis, we explored the design of multimers, which could provide such interactions. The design was investigated and revisited based on specific …
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Spatiotemporal Control of Actin Cytoskeletal Machinery in Cells: Multivalency Mediated Protein Clustering at the Plasma Membrane and Optogenetic Tool Development
… are largely unknown. Here I demonstrate that multivalent interactions among proteins in the Nephrin/Nck/N-WASP actin-regulatory pathway can drive clustering of the components at the plasma membrane in cells. Generation of the micronsized domains is dependent on the phosphorylation-mediated …
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Multivalent Programmable Interactions Between Lipid Vesicles: Towards Responsive Soft Materials
… with a variety of structures. More recently, multivalent interactions have been exploited to drive self-assembly of nanoparticles, hard colloids and compliant units including emulsion droplets and lipid vesicles. By applying this to deformable lipid vesicles, formation of links between two …
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Targeting HIV-1 RNAs with Medium Sized Branched Peptides Featuring Boron and Acridine-Branched Peptide Library Design, Synthesis, High-Throughput Screening and Validation
… interfaces for specific RNA-RNA or RNA-protein interactions that are essential for biological processes in a living system. These discoveries greatly increased interest in RNA as a potential drug target for the treatment of diseases. Two of the most studied RNA based regulatory systems are HIV-1 …
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Investigating processing body condensation, material properties and function during early Drosophila development
… condensates that are regulated by synergistic, multivalent interactions between structurally distinct protein and RNA molecules. I also demonstrate that the gel-like biophysical state of P bodies allows for the storage of bicoid, a key maternal transcript needed for embryonic development. Using …
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Carbon nanotube-based sensors for label-free protein detection
… description due to complexities associated with multivalent interactions. We show that a bivalent binding mechanism is able to describe this concentration dependence of KD,eff which varies from 100 pM to 1 uM for IgG concentrations from 1 ng/ml to 100 ug/ml, respectively. The mechanism is shown …
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Modelling multivalent interactons
A Multivalent entity, which could represent a protein, nanoparticle, polymer, virus or a lipid bilayer, has the ability to form multiple bonds to a substrate. Hence, a multivalent interaction can be strong, even if the individual bonds are weak. However, much more interestingly, multivalency …
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Engineering mono- and multi-valent inhibitors on a modular repeat-protein scaffold to target oncogenic Tankyrase
… the engineered TBP- CTPR proteins and TNKS are multivalent, and the effects of multivalency were explored both in the test tube and in the cell. The results show that the proteins interact to form large assemblies. Moreover, the TBP-CTPR proteins were found to have exceptional activity in …
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Unlocking precision medicine using DNA nanotechnology
… positioned using DNA origami structures, multivalent interactions can be tuned to specifically bind to a single target, akin to aptamers or antibodies. I present the design and experimental validation of a DNA origami framework that can precisely tune the position of ligands within an …
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Maximum Entropy Optimization: a General Approach to Study Ordered and Disordered Proteins Reveals Key Features of Protein Phase Separation
… condensates and help uncover physicochemical interactions that dictate their stability. However, the presence of both ordered and disordered domains in these proteins places a high demand on the model accuracy. In this thesis, we develop systematic methods to parameterize force fields from …
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Engineering Synthetic Biomolecular Condensates with Rationally-Designed Modular Repeat Proteins
… structured CTPR domains significantly modulate multivalent interactions among LCD-CTPR proteins, impacting condensate stability, phase boundaries, and material properties. Multiple fluorescence-based methods—including fluorescence recovery after photobleaching (FRAP), fluorescence lifetime …
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Mapping the synapse : synthesis and validation of chemical probes for interrogating PDZ domains
… higher eukaryotes functions in receptor-mediated multivalent interactions with partner PDZ domains. Electrophysiological data presented in this thesis suggest the effectiveness of the bivalent STG-2 peptide in the inhibition of the critical C. elegans GLR- 1 neural receptor complex.
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Functional divergence of the RNA polymerase II transcription machinery in Plasmodium falciparum
… the biochemical characterisation of protein-DNA interactions, protein-protein interactions and the biomolecular condensation properties of these transcription factors, this study aimed to provide insight into the molecular mechanisms governing promoter recognition and the regulated assembly of …
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Mechanistic Insights into the Microtubule-Mediated Differential Modulations of Aurora B Substrate Structures for Accurate Chromosome Segregation
… Aurora B phosphorylation sites, engages in multivalent interactions to support the oligomerizing conformation of Ndc80C on microtubules. This geometry reveals a microtubule-mediated substrate masking mechanism, in which key phosphorylation sites become inaccessible to the kinase, thereby …
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Dynamic-template-directed crystallization of semiconducting polymers for organic electronics
… is key to promoting template–polymer interactions. The result of such enhanced interaction is lower nucleation barrier and expedited crystallization process which is responsible for the improved morphological characteristics. I further established the dynamic-template-directed assembly …
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Regulation of WASP in Actin Signaling: From Angstroms to Microns
… WASP, which is itself autoinhibited through interactions between a regulatory GTPase binding domain (GBD) and an activity bearing VCA region. First, I investigated how a single repeat fragment (1R) from EspFU relieves WASP autoinhibition. By determining the solution structure of a complex …