Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"multiplexed imaging"”.
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Massively multiplexed imaging probes for comprehensive single-cell analysis
… detail. Recent efforts to combine physiological imaging and single-cell molecular analysis seek complete understanding of cellular identity and function within complex tissues. Specific cells of interest can be selected and isolated from tissues for downstream molecular analyses using techniques …
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Deciphering and modelling the action of immune cells using highly multiplexed imaging and deep learning techniques
… during infection, health, or chronic disease. Multiplexed imaging is a beneficial tool that is used to visualize distinct cell types and functional states directly in tissues. This technology is particularly important for understanding how cells organize spatially to enforce this boundary …
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Near-infrared emitting quantum dots for cellular and vascular fluorescent labeling in in vivo multiplexed imaging studies
In vivo multimodal, multiplexed microscopy allows real-time observation of hematopoietic cells, their stem and progenitor cells and metastatic cancer cells in their native bone marrow (BM) environment. Multiplexing has made possible detailed studies of the BM's microarchitecture, which helps define …
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Spatially organized fluorescent reporters for recording complex biological dynamics in cell population
… However, due to the diffraction limit of optical imaging, the biological signals recorded from neurons in densely-labeled neural populations in vivo are often mixed with signals from closely passing axons and dendrites from other neurons, resulting in erroneous signaling events and artifactual …
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Advanced imaging via multiplexed sensing and compressive sensing
This dissertation works on advanced imaging systems using multiplexed sensing and compressive sensing (CS). Conventional cameras (e.g., pin-hole and lens cameras) follow the one-object-point-to-one-image-point or one-to-one (OTO) mapping model. Multipled sensing and compressive sensing attempt to …
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Nanoscale biomolecular mapping in cells and tissues with expansion microscopy
… Microscopy (ExM) enables the 3D, nanoscale imaging of biological structures by physically magnifying cells and tissues. Specimens, embedded in a swellable hydrogel, undergo uniform expansion as covalently anchored labels and tags are isotropically separated. ExM thereby allows for the …
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Expansion microscopy of C. elegans : nanoscale imaging of biomolecules throughout an entire organism
… microscopy (ExM) enables 3-D, nanoscale-precise imaging of biological specimens by isotropic swelling of hydrogel-embedded, chemically processed tissue. Such capability raises the question of whether nanoscale mapping of biomolecules could be performed in an entire organism, which would allow …
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Expansion microscopy : scalable and multiplexed nanoscale imaging
… allowing for unprecedented super-resolution imaging in thick tissues and facile reagent diffusion into the sample. We have since developed a variant of ExM, called protein retention ExM, in which proteins are directly anchored to the swellable gel using a commercially available cross-linking …
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Through barrier detection using surface enhanced spatially offset Raman spectroscopy
In the fields of security and biomedical imaging there is a significant need to non-invasively probe through barriers, e.g. plastic, glass or tissue. Raman spectroscopy provides a means to solving this challenge since it provides a unique chemical fingerprint without the need to destroy the sample. …
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Source modulated multiplexed hyperspectral imaging: Theory, hardware and application
… that Wilcoxon without error can be estimated. Imaging of simple objects and signal to noise ratio (SNR) experiments are used to test the hyperspectral imager. The simple objects allow us to see that the imager produces sensible spectra. The experiments involve looking at the SNR itself and the …
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Clonal Phenotype Mapping Reveals Genomic Alterations Underlying Tumor Immunosuppression during Immunotherapy
<p>Tumors are dynamic ecosystems that evolve under selective pressures, shaping their ability to either elicit or evade immune responses. In pancreatic ductal adenocarcinoma (PDAC), where immunotherapy has yet to become an effective treatment option, we investigated the role of intratumoral …